Synergistic Interaction between Copper and Nitrogen-Uptake, Translocation, and Distribution in Rice Plant

Abstract

Interactions among nutrients have been widely recognized in plants and play important roles in crop growth and yield formation. However, the interplay of Cu and N in rice plants is not yet clear. In this study, rice plants were grown with different combinations of Cu and N supply. The effects of Cu-N interaction on the growth, yield production, Cu and N transport, and gene expression levels were analyzed. The results showed that the effect of N supply on rice growth and yield formation was more pronounced than that of Cu supply. The Cu supply significantly improved the uptake of N (by 9.52–30.64%), while the N supply significantly promoted the root-to-shoot translocation of Cu (by 27.28–38.45%) and distributed more Cu (1.85–19.16%) into the shoots and leaves. The results of qRT-PCR showed that +Cu significantly up-regulated the expression levels of both NO3− and NH4+ transporter genes OsNRTs and OsAMTs, including OsNRT1.1B, OsNRT2.1, OsNRT2.3a, OsNRT2.4, OsAMT1.2, OsAMT1.3, and OsAMT3.1. Meanwhile, +N significantly up-regulated the expression levels of Cu transporter genes OsHMA5 and OsYSL16. In addition, the supply of Cu up-regulated the expression levels of OsGS1;2, OsGS2, and OsNADH-GOGAT to 12.61-, 6.48-, and 6.05-fold, respectively. In conclusion, our study demonstrates a synergistic effect between Cu and N in rice plants. It is expected that our results would be helpful to optimize the application of N and Cu fertilizers in agriculture.

Impacts of cobalt and zinc on improving peanuts nutrient uptake, yield and irrigation water use efficiency under different irrigation levels

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Assessing Crude Protein Levels and Microelement Concentrations of Flour Grain Yield of Sorghum bicolor (L.) Moench Cultivars

“…Cu is the trace element with the lowest concentration among the analyzed element concentrations. The details of element accumulation in various sorghum cultivars were detailed in Figure 2; the result was linked to (Cui et al, 2022); the study showed that plants' copper and nitrogen assimilation, translocation, and distribution could interact effectively, as was evidenced in Figure 3, the correlation between Cu and Zn, r (5) = 66, P = 20.…”

Mechanochemical Synthesis of Nitrogen-Efficient Zn- and Cu-Sulfate Urea Cocrystals from Carbonate Minerals

“…We further focus on copper and zinc micronutrients, both shown to exhibit synergistic interactions with nitrogen toward plant uptake. The presence of copper improved the uptake of N in rice plants by 9−30%, 25 while zinc was shown as an essential micronutrient that is the most commonly deficient in plants, particularly in high-pH soils. 26 Only a few scant examples of Cu and Zn sulfate urea cocrystals exist in the literature, and all of them were synthesized using crystallization from aqueous solutions.…”