Synergistic infection of BrYV and PEMV 2 increases the accumulations of both BrYV and BrYV-derived siRNAs in Nicotiana benthamiana

Zhou, Cui-Ji; Zhang, Xiaoyan; Liu, Songyu; Wang, Ying; Li, Dawei; Yu, Jialin; Han, Chenggui

doi:10.1038/srep45132

Cited by 27 publications

(27 citation statements)

References 57 publications

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“…It has been also previously demonstrated that DCL4 can downregulate DCL2 expression [56]. [66]. These results are in agreement with our previous findings [51,54] It should take in account, however, that ours results to not exclude that other CLRDV-resistance mechanisms not directly related to DCL2/4 modulation should be active in DO plants.…”

Section: Discussion 14supporting

confidence: 93%

Genome-wide identification of the Dicer-like family in cotton and analysis of the DCL expression modulation in response to biotic stress in two contrasting commercial cultivars

Moura

Fausto

Fanelli

et al. 2019

Preprint

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Background Dicer-like proteins (DCLs) are fundamental players in RNA-silencing mechanisms acting in gene regulation via miRNAs and in antiviral protection in plants, in addition to being related to other biotic and abiotic stresses. Despite be identified in some crops, cotton DCLs weren't characterized until now. Here we characterize the DCLs of three cotton species and analyzed their expression profiles during biotic stress. Results We identified 11 DCLs in the allotetraploid cotton Gossypium hirsutum and 7 and 6 in the diploid G. arboreum and G. raimondii, respectively. Among some DCL duplication observed in these genomes, we observe the presence of an extra DCL3 in the three cotton species, not found in others eudicots until now. All the DCL types identified by in silico analysis in the allotetraploid cotton genome were able to generate transcripts, as observed by gene expression analysis in distinct tissues. Based on the importance of DCLs for plant virus defense, responses of cotton DCLs to virus infection and/or herbivore attack using two commercial cotton cultivars (cv.), one susceptible (Fibermax 966) and another resistant (Delta Opal) to polerovirus CLRDV infection were analyzed. Both cvs. responded differently to virus infection. At the initial stages, 24dpi, the resistant cv. showed strong induction of DCL2a and b, while the susceptible cv. showed a down-regulation of these genes, wherever DCL4 expression was highly induced. Herbivore attack did not induce contrasting profiles between cotton DCLs transcripts in either cotton cv. Conclusions The allotetraploide cotton G. hirsutum has almost all DCLs found in their diploid relative and duplication of DCL2 and DCL3 were found in the three species. All the four classes of DCL respond to aphid attack and virus infection in G. hirsutum, however, remarkable differences in DCL initial responses against the virus itself may be responsible for the virus susceptible and/or resistant phenotype of the contrasting cotton cv. studied.

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Section: Discussion 14supporting

confidence: 93%

Genome-wide identification of the Dicer-like family in cotton and analysis of the DCL expression modulation in response to biotic stress in two contrasting commercial cultivars

Moura

Fausto

Fanelli

et al. 2019

Preprint

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“…Brassica yellows virus (BrYV) is a tentative species in the genus Polerovirus . Viruses in this genus and Luteovirus are limited to the phloem and transmitted by aphid ( King et al, 2012 ; Zhou et al, 2017 ). BrYV is distributed widely in mainland China, as well as in South Korea and Japan ( Xiang et al, 2011 ; Zhang et al, 2014 ; Lim et al, 2015 ; Kamitani et al, 2016 ).…”

Section: Introductionmentioning

confidence: 99%

“…For convenience of the virus research, the full-length infectious cDNA clones of BrYVs were constructed under control of the cauliflower mosaic virus 35S promoter ( Zhang et al, 2015 ). As the BrYV is a phloem-restricted virus, an umbravirus Pea enation mosaic virus 2 (PEMV 2) can help BrYV to be mechanically transmitted and invade non-vascular tissue in Nicotiana benthamiana ( Zhou et al, 2017 ).…”

Section: Introductionmentioning

confidence: 99%

The Conserved Proline18 in the Polerovirus P3a Is Important for Brassica Yellows Virus Systemic Infection

Zhang

Zhao

et al. 2018

Front. Microbiol.

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ORF3a, a newly identified non-AUG-initiated ORF encoded by members of genera Polerovirus and Luteovirus, is required for long-distance movement in plants. However, the mechanism of action of P3a in viral systemic movement is still not clear. In this study, sequencing of a brassica yellows virus (BrYV) mutant defective in systemic infection revealed two-nucleotide variation at positions 3406 and 3467 in the genome. Subsequent nucleotide substitution analysis proved that only the non-synonymous substitution (C→U) at position 3406, resulting in P3aP18L, abolished the systemic infection of BrYV. Preliminary investigation showed that wild type BrYV was able to load into the petiole of the agroinfiltrated Nicotiana benthamiana leaves, whereas the mutant displayed very low efficiency. Further experiments revealed that the P3a and its mutant P3aP18L localized to the Golgi apparatus and near plasmodesmata, as well as the endoplasmic reticulum. Both P3a and P3aP18L were able to self-interact in vivo, however, the mutant P3aP18L seemed to form more stable dimer than wild type. More interestingly, we confirmed firstly that the ectopic expression of P3a of other poleroviruses and luteoviruses, as well as co-infection with Pea enation mosaic virus 2 (PEMV 2), restored the ability of systemic movement of BrYV P3a defective mutant, indicating that the P3a is functionally conserved in poleroviruses and luteoviruses and is redundant when BrYV co-infects with PEMV 2. These observations provide a novel insight into the conserved function of P3a and its underlying mechanism in the systemic infection.

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“…Therefore, it is assumed that DCL2 and DCL4 likely play important role in generating WLYaV siRNAs whereas in most viruses and viroids, the 21 nt species is the predominant class (Donaire et al, 2009 ; Ma et al, 2011 ; Li et al, 2012 ; Zhang et al, 2014 ). For poleroviruses such as cotton leafroll dwarf virus, MaYMV, and brassica yellows virus, the abundant vsiRNA class is 22 nt (Silva et al, 2011 ; Chen et al, 2016 ; Zhou et al, 2017 ). This suggests that these poleroviruses and WLYaV may affect DCL4 activity which is then compensated by DCL2.…”

Section: Discussionmentioning

confidence: 99%

Identification, Characterization and Full-Length Sequence Analysis of a Novel Polerovirus Associated with Wheat Leaf Yellowing Disease

Zhang

Liu

et al. 2017

Front. Microbiol.

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To identify the pathogens responsible for leaf yellowing symptoms on wheat samples collected from Jinan, China, we tested for the presence of three known barley/wheat yellow dwarf viruses (BYDV-GAV, -PAV, WYDV-GPV) (most likely pathogens) using RT-PCR. A sample that tested negative for the three viruses was selected for small RNA sequencing. Twenty-five million sequences were generated, among which 5% were of viral origin. A novel polerovirus was discovered and temporarily named wheat leaf yellowing-associated virus (WLYaV). The full genome of WLYaV corresponds to 5,772 nucleotides (nt), with six AUG-initiated open reading frames, one non-AUG-initiated open reading frame, and three untranslated regions, showing typical features of the family Luteoviridae. Sequence comparison and phylogenetic analyses suggested that WLYaV had the closest relationship with sugarcane yellow leaf virus (ScYLV), but the identities of full genomic nucleotides and deduced amino acid sequence of coat protein (CP) were 64.9 and 86.2%, respectively, below the species demarcation thresholds (90%) in the family Luteoviridae. Furthermore, agroinoculation of Nicotiana benthamiana leaves with a cDNA clone of WLYaV caused yellowing symptoms on the plant. Our study adds a new polerovirus that is associated with wheat leaf yellowing disease, which would help to identify and control pathogens of wheat.

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Synergistic infection of BrYV and PEMV 2 increases the accumulations of both BrYV and BrYV-derived siRNAs in Nicotiana benthamiana

Cited by 27 publications

References 57 publications

Genome-wide identification of the Dicer-like family in cotton and analysis of the DCL expression modulation in response to biotic stress in two contrasting commercial cultivars

Genome-wide identification of the Dicer-like family in cotton and analysis of the DCL expression modulation in response to biotic stress in two contrasting commercial cultivars

The Conserved Proline18 in the Polerovirus P3a Is Important for Brassica Yellows Virus Systemic Infection

Identification, Characterization and Full-Length Sequence Analysis of a Novel Polerovirus Associated with Wheat Leaf Yellowing Disease

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