Synergistic in vitro antimalarial activity of plant extracts used as traditional herbal remedies in Mali

Azas, Nadine; Laurencin, N.; Delmas, F.; Giorgio, Carole Di; Gasquet, M.; Laget, M.; Timon‐David, P.

doi:10.1007/s004360100454

Cited by 102 publications

(76 citation statements)

References 15 publications

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“…Based on the methodology of Azas et al (2002), the standard isobolar analysis was used to evaluate the combination effects of the methanolic extract of Acanthospermum hispidum (being the more active extract) and chloroquine on both 3D7 and Dd2 strains. Following the procedure described by Berenbaum (1978), various combination mixtures of the extract and chloroquine (different ratio: 5:0, 4:1, 3:2, 2.5:2.5, 2:3, 1:4 and 0:5 of their respective IC 50 s determined beforehand) were tested using 96-well microtiter plates.…”

Section: Drug Interaction Assaymentioning

confidence: 99%

In vitro evaluation of antiplasmodial activity of extracts of Acanthospermum hispidum dc (Asteraceae) and Ficus thonningii blume (moraceae), two plants used in traditional medicine in the republic of Congo

Koukouikila-Koussounda¹,

Abena²,

Nzoungani³

et al. 2013

Afr. J. Trad. Compl. Alt. Med.

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The aim of this study was to evaluate extracts from two medicinal plants, Acanthospermum hispidum and Ficus thonningii, used in traditional medicine in Congo Brazzaville, for in vitro antiplasmodial activities against two laboratory strains of Plasmodium falciparum: the chloroquine sensitive 3D7 and the chloroquine resistant Dd2. ELISA HRP2 assay was used to evaluate the in vitro inhibitory activity of the extracts alone or in combination with chloroquine. Cytotoxicity was assessed on human HeLa cell line and reflected by the selectivity index. Methanolic extract of Acanthospermum hispidum exhibited a strong and a moderate inhibitory activity on the growth of Dd2 and 3D7 at 2.8 µg/ml and 9.2 µg/ml concentrations respectively with a selectivity index >10. The combination of the most active extract (methanolic extract of Acanthospermum hispidum) with chloroquine showed a synergistic interaction on both strains. The good selectivity index of Acanthospermum hispidum on HeLa cells reflects the safety of this plant. Extracts from Ficus thonningii did not show any promising antiplasmodial activity on both 3D7 and Dd2. Except the methanolic extract which exhibited a slight antiplasmodial activity with inhibitory concentration and selectivity index corresponding to 9.61 µg/ml and 11.16 respectively. Methanolic extract of Acanthospermum hispidum exhibited moderate to high inhibitory activity on 3D7 and Dd2 laboratory strains and a synergistic antimalarial effect when combined with chloroquine. Ficus thonningii seems to have no antimalarial activity. Phytochemical analysis, in vivo investigations using animal models and later clinical trials in collaboration with traditional practitioners are necessary to clarify the potential antimalarial activity of both plants.

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Section: Drug Interaction Assaymentioning

confidence: 99%

In vitro evaluation of antiplasmodial activity of extracts of Acanthospermum hispidum dc (Asteraceae) and Ficus thonningii blume (moraceae), two plants used in traditional medicine in the republic of Congo

Koukouikila-Koussounda¹,

Abena²,

Nzoungani³

et al. 2013

Afr. J. Trad. Compl. Alt. Med.

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“…Traditional healers seem to have already taken advantage of enhanced efficacy of combined decoctions. For example, in Mali, Nauclea latifolia was associated with Mitragyna inermis or Guiera senegalensis and Feretia apodanthera with Mitragyna inermis (Azas et al, 2004). Since total extracts were used, the bioactive compounds should be isolated and their interactions investigated.…”

Section: In Vivo Drug Interactionsmentioning

confidence: 99%

The in vitro anti-plasmodial and in vivo anti-malarial efficacy of combinations of some medicinal plants used traditionally for treatment of malaria by the Meru community in Kenya

Gathirwa

Rukunga

Njagi

et al. 2008

Journal of Ethnopharmacology

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The use of herbal drugs as combinations has existed for centuries in several cultural systems. However, the safety and efficacy of such combinations have not been validated. In this study, the toxicity, anti-plasmodial and antimalarial efficacy of several herbal drug combinations were investigated. Lannea schweinfurthii, Turraea robusta and Sclerocarya birrea, used by traditional health practitioners in Meru community, were tested for in vitro anti-plasmodial and in vivo anti-malarial activity singly against Plasmodium falciparum and Plasmodium berghei, respectively. Methanolic extract of Turraea robusta was the most active against Plasmodium falciparum D6 strain. Aqueous extracts of Lannea schweinfurthii had the highest anti-plamodial activity followed by Turraea robusta and Sclerocarya birrea. D6 was more sensitive to the plant extracts than W2 strain. Lannea schweinfurthii extracts had the highest anti-malarial activity in mice followed by Turraea robusta and Sclerocarya birrea with the methanol extracts being more active than aqueous ones. Combinations of aqueous extracts of the three plants and two others (Boscia salicifolia and Rhus natalensis) previously shown to exhibit anti-plasmodial and anti-malarial activity singly were tested in mice. Marked synergy and additive interactions were observed when combinations of the drugs were assayed in vitro. Different combinations of Turraea robusta and Lannea schweinfurthii exhibited good in vitro synergistic interactions. Combinations of Boscia salicifolia and Sclerocarya birrea; Rhus natalensis and Turraea robusta; Rhus natalensis and Boscia salicifolia; Turraea robusta and Sclerocarya birrea; and Lannea schweinfurthii and Boscia salicifolia exhibited high malaria parasite suppression (chemo-suppression >90%) in vivo when tested in mice. The findings are a preliminary demonstration of the usefulness of combining several plants in herbal drugs, as a normal practice of traditional health practitioners.

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“…n Africa, the majority of the population does not have access to drug therapy but has access to traditional medicine for treating fevers (2). Guiera senegalensis is a shrub found in the savannah region of West and Central Africa.…”

mentioning

confidence: 99%

Harmine Is a Potent Antimalarial Targeting Hsp90 and Synergizes with Chloroquine and Artemisinin

Shahinas

MacMullin

Benedict

et al. 2012

Antimicrob Agents Chemother

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Previous studies have shown an antimalarial effect of total alkaloids extracted from leaves of Guiera senegalensis from Mali in West Africa. We independently observed that the beta-carboline alkaloid harmine obtained from a natural product library screen inhibited Plasmodium falciparum heat shock protein 90 (PfHsp90) ATP-binding domain. In this study, we confirmed harmine-PfHsp90-specific affinity using surface plasmon resonance analysis (dissociation constant [K d ] of 40 M). In contrast, the related compound harmalol bound human Hsp90 (HsHsp90) (K d of 224 M) more tightly than PfHsp90 (K d of 7,010 M). Site-directed mutagenesis revealed that Arg98 in PfHsp90 is essential for harmine selectivity. In keeping with our model indicating that Hsp90 inhibition affords synergistic combinations with existing antimalarials, we demonstrated that harmine potentiates the effect of chloroquine and artemisinin in vitro and in the Plasmodium berghei mouse model. These findings have implications for the development of novel therapeutic combinations that are synergistic with existing antimalarials.

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Synergistic in vitro antimalarial activity of plant extracts used as traditional herbal remedies in Mali

Cited by 102 publications

References 15 publications

<i>In vitro</i> evaluation of antiplasmodial activity of extracts of <i>Acanthospermum hispidum</i> dc (Asteraceae) and <i>Ficus thonningii</i> blume (moraceae), two plants used in traditional medicine in the republic of Congo

<i>In vitro</i> evaluation of antiplasmodial activity of extracts of <i>Acanthospermum hispidum</i> dc (Asteraceae) and <i>Ficus thonningii</i> blume (moraceae), two plants used in traditional medicine in the republic of Congo

The in vitro anti-plasmodial and in vivo anti-malarial efficacy of combinations of some medicinal plants used traditionally for treatment of malaria by the Meru community in Kenya

Harmine Is a Potent Antimalarial Targeting Hsp90 and Synergizes with Chloroquine and Artemisinin

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