Synergistic effects of verapamil on pingyangmycin‐induced cytotoxicity and apoptosis in KB cells

Yc, Ho; Kw, Tai; Yc, Chang

doi:10.1111/j.1601-0825.2006.01242.x

Cited by 3 publications

(2 citation statements)

References 22 publications

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“…Pingyangmycin has been shown to exhibit cytotoxicity against numerous types of tumor cells, including tongue carcinoma, sacrococcygeal chordoma and oral carcinoma cells (18,19). The induction of apoptosis constitutes an important mechanism for the eradication of tumor cells by therapeutic agents.…”

Section: Discussionmentioning

confidence: 99%

Pingyangmycin stimulates apoptosis in human hemangioma-derived endothelial cells through activation of the p53 pathway

Jiang

et al. 2014

Molecular Medicine Reports

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Abstract. Pingyangmycin (also known as Bleomycin A5) is produced by Streptomyces verticillus var. pingyangensis n.sp., and has anti-tumor activities against a variety of tumor cells. The aim of the present study was to determine the molecular mechanism(s) underlying the therapeutic effects of pingyangmycin against infantile hemangiomas. Human hemangioma-derived endothelial cells (HemECs) were treated with pingyangmycin at varying concentrations (100, 200 or 300 µg/ml), and the morphological changes and apoptosis levels were assessed. The gene expression changes were determined by cDNA microarray technology. Transmission electron microscopy examination revealed that the pingyangmycin-treated HemECs exhibited typical apoptotic characteristics, including chromatin condensation and the formation of apoptotic bodies. Annexin-V staining demonstrated that pingyangmycin caused a significant and dose-dependent induction of apoptosis in the HemECs. In the pingyangmycin-treated HemECs, 4,752 genes demonstrated at least 2-fold expression changes at the mRNA level. Quantitative polymerase chain reaction confirmed that pingyangmycin significantly upregulated the expression of p53, p53-induced protein with death domain, Bax, p53 upregulated modulator of apoptosis and p53 inducible gene 3, and downregulated the expression of murine double minute 2. The data demonstrated that the pro-apoptotic activity of pingyangmycin against infantile hemangiomas involves p53 pathway activation.

show abstract

Section: Discussionmentioning

confidence: 99%

Pingyangmycin stimulates apoptosis in human hemangioma-derived endothelial cells through activation of the p53 pathway

Jiang

et al. 2014

Molecular Medicine Reports

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show abstract

“…Cell cycle analysis was performed on the cells stained with propidium iodine as described by previously (14,15). Controlled cells and butyrate-treated cells were detached by treating them with 0.05% trypsin and then washed twice with cold phosphate-buffered saline (PBS) and fixed in 80% ethanol in PBS at Ϫ20°C.…”

Section: Cell-cycle Analysismentioning

confidence: 99%

Effects of a Bacterial Lipid Byproduct on Human Pulp Fibroblasts In Vitro

Chang

2007

Journal of Endodontics

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Delivery of bleomycin A5 into cells using TiO2 nanoparticles to enhance the degradation of intracellular DNA

et al. 2013

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Synergistic effects of verapamil on pingyangmycin‐induced cytotoxicity and apoptosis in KB cells

Abstract: Verapamil was found to enhance PYM-induced cytotoxicity and apoptosis in KB cells. The responsiveness of PYM might be explained by the effective accumulation of PYM by verapamil in KB cells mediated by the inhibition of PYM efflux function of the cells.

Cited by 3 publications

References 22 publications

Pingyangmycin stimulates apoptosis in human hemangioma-derived endothelial cells through activation of the p53 pathway

Pingyangmycin stimulates apoptosis in human hemangioma-derived endothelial cells through activation of the p53 pathway

Effects of a Bacterial Lipid Byproduct on Human Pulp Fibroblasts In Vitro

Delivery of bleomycin A5 into cells using TiO2 nanoparticles to enhance the degradation of intracellular DNA

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