Synergistic effects of pH shift and heat treatment on solubility, physicochemical and structural properties, and lysinoalanine formation in silkworm pupa protein isolates

Revealing the synergistic effect of hydration and pulsed ultrasound on the emulsifying properties of silkworm pupa protein and its stabilized emulsion

“…1B). The amide I band (1700–1600 cm −1 ) 25 and amide III band (1350–1200 cm −1 ) 1 were observed in the spectra. The peak intensity of amide I of the sample hydrated for the same time increased, and a blueshift of the peak at 1672 cm −1 was observed after the different ultrasound treatments.…”

“…The peaks of the amide III band were assigned as follows: ⊍-helix (1330-1295 cm −1 ), ⊎-turn (1295-1270 cm −1 ), random coil (1270-1250 cm −1 ) and ⊎-sheet (1250-1220 cm −1 ). 1 The peak intensity of SPP at 1238 cm −1 was increased after ultrasound treatment (Fig. 1B), suggesting a significant alteration in the ⊎-sheet structure of the treated SPP.…”

“…Considering that the amide I band was highly susceptible to interference caused by water vibrations, 27 the second structure of SPP was investigated through vibrational frequency in the amide III band. The peaks of the amide III band were assigned as follows: α ‐helix (1330–1295 cm −1 ), β ‐turn (1295–1270 cm −1 ), random coil (1270–1250 cm −1 ) and β ‐sheet (1250–1220 cm −1 ) 1 . The peak intensity of SPP at 1238 cm −1 was increased after ultrasound treatment (Fig.…”

“…Silkworm ( Bombyx moil L.) pupa (SP) is the main byproduct of the silk reeling industry. China, as the second largest silk producer in Asian countries, 1 yields more than 500 000 tons of SP each year 2 . SP protein (SPP), which represents 49–54% of the total SP weight, is isolated from SP.…”

Flexible processing technology of coix seed prolamins by combined heat-ultrasound: Effects on their enzymatic hydrolysis characteristics and the hypoglycemic activities of derived peptides

“…2 B ). Under the HT or US treatment conditions, coix seed prolamin molecular particles underwent cleavage and the frequency of intermolecular collisions increases, all of which favour a reduction in protein molecular particle size [43] . Compared with the HT group, the HT + US treatment separated the thermally aggregated coix seed prolamin particles, and the protein particles were more evenly distributed and finer in size ( Fig.…”