Synergistic Effects of PectaSol-C Modified Citrus Pectin an Inhibitor of Galectin-3 and Paclitaxel on Apoptosis of Human SKOV-3 Ovarian Cancer Cells

Hossein, Ghamartaj; Keshavarz, Maryam; Ahmadi, Samira; Naderi, Nima

doi:10.7314/apjcp.2013.14.12.7561

Cited by 53 publications

(41 citation statements)

References 36 publications

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“…The mitochondria membrane potential (MMP) was analyzed by using a JC-1 (5, 5', 6, 6'-tetrachloro-1, 1', 3, 3-tetraethylbenzimidazolocarbocyanine Iodide) fluorescence probe kit (Beyotime, China) as described previously (Hossein et al, 2013). The lipophilic and cationic fluorescent dye JC-1 is capable of selectively entering mitochondria, where it forms aggregates and emits red fluorescence when MMP is high.…”

Section: Mitochondria Membrane Permeability Assaymentioning

confidence: 99%

Curcumin Induces Apoptosis in SGC-7901 Gastric Adenocarcinoma Cells via Regulation of Mitochondrial Signaling Pathways

Xue¹,

Yu²,

Sun³

et al. 2014

Asian Pacific Journal of Cancer Prevention

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Curcumin, a polyphenol compound derived from the rhizome of the plant Curcuma longa L. has been verified as an anticancer compound against several types of cancer. However, understanding of the molecular mechanisms by which it induces apoptosis is limited. In this study, the anticancer efficacy of curcumin was investigated in human gastric adenocarcinoma SGC-7901 cells. The results demonstrated that curcumin induced morphological changes and decreased cell viability. Apoptosis triggered by curcumin was visualized using Annexin V-FITC/7-AAD staining. Curcumin-induced apoptosis of SGC-7901 cells was associated with the dissipation of mitochondrial membrane potential (MMP) and the release of cytochrome c into the cytosol. Furthermore, the down-regulation of Bcl-2 and up-regulation of Bax that led to the cleavage of caspase-3 and increased cleaved PARP was observed in SGC-7901 cells treated with curcumin. Therefore, curcumin-induced apoptosis of SGC-7901 cells might be mediated through the mitochondria pathway, which gives the rationale for in vivo studies on the utilization of curcumin as a potential cancer therapeutic compound.

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Section: Mitochondria Membrane Permeability Assaymentioning

confidence: 99%

Curcumin Induces Apoptosis in SGC-7901 Gastric Adenocarcinoma Cells via Regulation of Mitochondrial Signaling Pathways

Xue¹,

Yu²,

Sun³

et al. 2014

Asian Pacific Journal of Cancer Prevention

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“…However, the molecular mechanisms of the anti-tumor effects of CSBE are still not clearly understood. Induction of apoptosis may be one of the possible mechanisms of tumor prevention (Banjerdpongchai et al, 2008;Rasul et al, 2011;Inan et al, 2012;Hossein et al, 2013;Banjerdpongchai and Khaw-On, 2013;Alabsi et al, 2013). Apoptosis is induced by activation of a series of intracellular signaling events such as mitochondrial cytochrome c release, and activation of caspase proteases (Thompson, 1995;Chiarugi and Moskowitz, 2002).…”

Section: Discussionmentioning

confidence: 99%

N-Butanol Extract of Capparis spinosa L. Induces Apoptosis Primarily Through a Mitochondrial Pathway Involving mPTP Open, Cytochrome C Release and Caspase Activation

Yu-bin

Yu²

2014

Asian Pacific Journal of Cancer Prevention

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“…The incidence of ovarian cancer has increased annually during the past two decades (17). The early stages of ovarian cancer can be effectively treated with chemotherapy; however, ~70% of ovarian cancers are diagnosed at a late stage (18), which severely effects the life quality and mortality rates of patients, since the cancer cells develop resistance to drugs, such as PTX (19). Cyto-reductive surgery is the first-line therapy for patients with all stages of ovarian cancer, which is used in combination with chemotherapy or radiotherapy for patients with late stage or recurrent ovarian cancer, so as to improve their quality of life and chances of survival (20).…”

Section: Discussionmentioning

confidence: 99%

“…Yang et al (25) previously demonstrated that luteolin could enhance PTX-induced apoptosis in MDA-MB-231 human breast cancer cells, by blocking signal transducer and activator of transcription 3. Hossein et al (19) showed that PectaSol-C modified citrus pectin could sensitize ovarian cancer cells to PTX by inducing apoptosis, which may lead to an accumulation of cells in the subG 1 and G 1 phases, and cleavage of caspase-3 (24)(25)(26)(27). Numerous studies have reported that CZEO is a promising antitumor drug, which has a direct cytotoxic effect that inhibits tumor cell growth and proliferation, disrupts nuclear metabolism, inhibits angiogenesis and impairs the membrane potential, all of which can be lethal to cancer cells (3,15,28).…”

Section: Discussionmentioning

confidence: 99%

Curcuma zedoaria (Berg.) Rosc. essential oil and paclitaxel synergistically enhance the apoptosis of SKOV3 cells

Zhou

Shen

Xia

et al. 2012

Molecular Medicine Reports

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Abstract. Curcuma zedoaria (Berg.) Rosc. essential oil (CZEO) is the major component of Curcuma zedoaria (Berg.) Rosc., a traditional medicine with antitumor activity. Paclitaxel (PTX) is a first-line chemotherapeutic agent used to treat patients with ovarian cancer. These compounds directly target nuclear DNA, in order to suppress or inhibit tumor cell growth. The present study aimed to determine the synergistic antitumor effects of CZEO and PTX on the SKOV3 human ovarian cancer cell line. SKOV3 cells were treated with CZEO, PTX or a combination of the two and cell viability was detected using cell counting kit-8. In addition, flow cytometry was used to determined cell apoptosis as well as for cell cycle analysis. The morpho logical changes of apoptosis were assessed using Hoechst 33342 staining and the expression levels of apoptotic pathway proteins, including caspase-3 and poly (ADP-ribose) polymerase (PARP), were quantified using western blot analysis. The cell viability assay indicated that either of these compounds alone or in combination suppressed the growth of SKOV3 cells. Furthermore, flow cytometric analysis indicated that treatment with a combination of CZEO and PTX resulted in increased inhibition of proliferation and induction of apoptosis of SKOV3 cells, as compared with treatment with either of the compounds alone. In addition, the protein expression levels of caspase-3 were increased following treatment with a combination of CZEO and PTX. The results of the present study suggested that CZEO and PTX synergistically enhanced the inhibition of SKOV3 proliferation, and the possible underlying mechanism may be the induction of cell apoptosis and cell cycle arrest. This therefore indicated that PTX supplemented with CZEO may be an effective treatment strategy to decrease the dose and toxicity of PTX.

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Synergistic Effects of PectaSol-C Modified Citrus Pectin an Inhibitor of Galectin-3 and Paclitaxel on Apoptosis of Human SKOV-3 Ovarian Cancer Cells

Cited by 53 publications

References 36 publications

Curcumin Induces Apoptosis in SGC-7901 Gastric Adenocarcinoma Cells via Regulation of Mitochondrial Signaling Pathways

Curcumin Induces Apoptosis in SGC-7901 Gastric Adenocarcinoma Cells via Regulation of Mitochondrial Signaling Pathways

N-Butanol Extract of Capparis spinosa L. Induces Apoptosis Primarily Through a Mitochondrial Pathway Involving mPTP Open, Cytochrome C Release and Caspase Activation

Curcuma zedoaria (Berg.) Rosc. essential oil and paclitaxel synergistically enhance the apoptosis of SKOV3 cells

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