Synergistic effects of multiple treatments, and both DNA and RNA direct bindings on, green tea catechins

Abstract

This article reviews two main topics: (1) the synergistic effects of multiple treatments with green tea catechin and (2) the direct binding of (-)-epigallocatechin gallate (EGCG) to both DNA and RNA molecules. Japanese drink green tea throughout the day, so we studied whether multiple treatments of cells with EGCG would enhance the expression of apoptosis-related genes, such as growth arrest and DNA damage-inducible gene (GADD153) and cyclin-dependent kinase inhibitor gene (p21(waf1)): The results suggest that the synergistic enhancement of both GADD153 and p21(waf1) gene expressions by multiple treatments plays a significant role in human cancer prevention with green tea beverage. Our previous observation-that nucleic acids extracted from catechin-treated cells are colored-allowed us to speculate that catechins directly interact with nucleic acids. Surface plasmon resonance assay (Biacore) indicated that four catechins, EGCG, (-)-epicatechin gallate (ECG), (+)-gallocatechin gallate (GCG), and (+)-catechin gallate (CG), bound to DNA oligomers. Cold spray ionization mass spectrometry (CSI-MS) analysis showed that one to three EGCG molecules bound to single-stranded 18 mers of DNA and RNA. Moreover, one or two molecules of EGCG bound to double-stranded AG:CT oligomers of various nucleotide lengths. Double-stranded DNA (dsDNA) oligomers were detected only as EGCG-bound forms at high temperature, whereas at low temperature both the free and bound forms were detected, suggesting that EGCG protects double-stranded DNA oligomers from double-stranded melting into single-stranded DNA. We assume that catechins accumulate in both double-stranded DNA and RNA molecules through multiple administrations of green tea beverage in in vivo, and that the accumulated green tea catechins play a significant role for human cancer prevention.

RPSA Gene Mutants Associated with Risk of Colorectal Cancer among the Chinese Population

“…A number of studies (Kuzuhara et al, 2007;Hakim et al, 2008;Kawai et al, 2008) had demonstrated that tea drinking was a protective factor for CRC risk. The most active constituents of green tea were EGCG, which accounted for almost 50% of the total catechin content in green tea extract, and it had very potent antioxidant effects.…”

Protective Effect of Persimmon (Diospyros kaki) Peel Proanthocyanidin against Oxidative Damage under H2O2-Induced Cellular Senescence

“…It was suggested that galloyl and catechol groups are essential for DNA binding. [34][35][36] Therefore, the structural difference between oligomers and polymers is considered to be an important factor regarding proanthocyanidin's action. Proanthocyanidin differs from all other natural polyphenols by virtue of its polymeric nature.…”

Generation of Mouse Monoclonal Antibody against (-)-Epigallocatechin Gallate

“…[11][12][13] The TG38 monoclonal antibody can be further utilized for basic and clinical experiments in cancer prevention, e.g., the monoclonal antibody makes it possible to investigate the metabolism of EGCG in serum and the distribution of EGCG in tissues and cells.…”

“…Moreover, prevention of high-grade prostate intraepithelial neoplasia was recently reported with green tea catechins. 10) Since EGCG was found to bind to numerous target molecules including DNA and RNA, 11,12) we theorized that green tea catechins induce cancer preventive activity mediated through a chaperone-like property. 13) We thus needed to study the bioavailability of EGCG and the presence of catechins in cells and in vivo.…”