Synergistic effects of inhibitors and osmotic stress during high gravity bioethanol production from steam-exploded lignocellulosic feedstocks

Hoppert, Luis; Kölling, Ralf; Einfalt, Daniel

doi:10.1016/j.bcab.2022.102414

Cited by 7 publications

(1 citation statement)

References 56 publications

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“…There are two main reasons why monitoring the concentrations of HMF and furfural is relevant: (i) because they are the products of degradation of hexoses and pentoses, respectively, their presence implies the loss of fermentable sugars, and (ii) depending on their concentration levels, these compounds can act as an inhibitor to the metabolism of a wide range of fermenting microorganisms [40][41][42][43][44].…”

Section: Furans Formationmentioning

confidence: 99%

Multi-Response Optimization of Thermochemical Pretreatment of Soybean Hulls for 2G-Bioethanol Production

Gil Rolón,

Leonardi,

Bolzico

et al. 2023

Fermentation

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Soybean is a major crop used in the production of human food. The soybean hull (SH) is also known as the seed coat and it constitutes about 5–8% of the total seed on a dry weight basis, depending on the variety and the seed size. Dilute sulfuric acid was employed for the thermochemical pretreatment of SH prior to enzymatic saccharification and alcoholic fermentation. Empirical modeling of response surface, severity factor and multi-response desirability function methodology, were used to perform the process optimization. Temperature, acid concentration and reaction time were defined as operational variables, while furfural, 5-hydroxymethylfurfural and solubilized hemicellulose and cellulose were defined as response variables. Mathematical models satisfactorily described the process and optimal conditions were found at 121 °C; 2.5% w/v H2SO4 and 60 min. More than 80% and 90% of hemicelluloses and celluloses, respectively, were able to solubilize at this point. The fermentation performance of an industrial Saccharomyces cerevisiae strain was also evaluated. The glucose available in the hydrolysates was completely consumed in less than 12 h, with an average ethanol yield of 0.45 gethanol/gglucose. Thus, the thermochemical conditioning of SH with dilute sulfuric acid is a suitable operation for 2G-bioethanol production.

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