Synergistic effects of Nepeta menthoides and Melissa officinalis aqueous extracts on reserpine‐induced depressive‐like behaviors in mice

Abstract

Nepeta menthoides Boiss. & Buhse and Melissa officinalis are extensively used in Persian medicine for the treatment of depression. Considering the active ingredients and main phenolic compounds of these plants and possible synergistic effects, this study examined the antidepressant and antioxidant activities of aqueous extract of N. menthoides (NM) and M. officinalis (MO) in reserpinized mice alone and combination. Mice were pretreated orally for 1‐week with normal saline (10 ml/kg), fluoxetine (20 mg/kg), imipramine (10 mg/kg), NM (50–100–200–400 mg/kg), MO (150–350–550–750 mg/kg), and combination (NM 50 with MO 150 mg/kg). The behavioral changes were evaluated using forced swim, tail suspension, and open field tests, 24 hr after reserpine injection (4 mg/kg) on eighth day. The amounts of active components in the extracts and catalase (CAT) as a brain oxidative stress were measured with enzyme‐linked immunosorbent assay. Data showed that this combination produced a synergistic action on behaviors and a significant increase in CAT activity. High‐performance liquid chromatography results showed that rosmarinic acid contents in MO and NM were 6.42 ± 1.1 and 11.03 ± 2.16 mg/g of dried extract, respectively. Total flavonoid and phenolic contents of MO were higher than NM. The findings suggest that the present combination produces an antidepressant‐like effect, which is possibly triggered by its antioxidant properties.

Exploring the Antidiabetic and Antioxidant Potential of Nepeta Adenophyta Hedge in Hepg2 Cells and Its Phytochemical Studies

“…Hz), 0.83 (3H, t, J = 6.9 Hz), 0.64 (3H, s). 13 values [20] and the structure of the pimarane diterpenoid was deduced as (2R,4aS,4bS,8aS)- The experimental data was found in correlation with previous literature [22]. 1…”

“…Derivatives of most pimarane diterpenoid have shown promising biological properties i.e., antitumor, anti-inflammatory, antimicrobial [12]. Recently, Nepeta species have been reported to treat depression [13],…”

“…1H, m, H-5), 0.89 (3H, s, H-18), 0.84 (3H, s, H-19), 0.78 (3H, s, H-20), 0.75 (3H, s, H-17) 13. C-NMR (DMSO-d6, 100 MHz): δC 148.5 (CH, C-15), 135.3 (C-8), 120.5 (CH, C-7), 111.3 (CH2, C-16), 73.CH, C-12), 69.4 (CH, C-1), 45.2 (CH2, C-14), 43.CH, C-5), 42.0 (C-13), 41.3 (CH, C-9), 39.0 (C-10), 34.1 (CH2, C-3), 33.8 (CH3, C-19), 32.5 (C-4), 28.CH2, C-11), 25.9 (CH2, C-2), 23.4 (CH2, C-6), 23.0 (CH3, C-18), 15.4 (CH3, C-17), 15.2 (CH3, C-20).…”

“…13 C NMR (400 MHz, DMSO-d6): δC 182.6 (CO, C-4), 164.4 (C-2), 161.7 (C-4ʹ), 159.0 (C-7), 153.0 (C-9), 152.5 (C-5), 132.3 (C-6), 128.8 (2CH, C-2ʹ, 6ʹ), 121.5 (C-1ʹ), 116.3 (2CH, C-3ʹ, 5ʹ), 105.5 (C-10), 103.0 (CH, C-3), 91.8 (CH, C-8), 60.4 (OCH3, C-6), 56.8 (OCH3, C-7).…”

Synthesis of blue-emitting polyvinylpyrrolidone coated copper nanoclusters for sensitive detection of sodium dichloroisocyanurate and rosmarinic acid

“…And flow injection analysis (FIA) and chemiluminescence analysis were used for detection of hypochlorous acid [15,16]. But there are many techniques for detecting RA concentrations, such as high-performance liquid chromatography (HPLC) [17], high-performance thin-layer chromatography (HPTLC) [18], differential pulse voltammetry (DPV) [19], ultraviolet-visible spectroscopy [12], and so on. However, these methods may have some problems, such as requiring complex sample preparation, specialized operators to operate instruments, time-consuming, or low sensitivity.…”