Synergistic effects of glial cell line‐derived neurotrophic factor and base‐medium on in vitro culture of testicular tissue derived from prepubertal collared peccary
Andreia Maria da Silva,
Ana Glória Pereira,
Luana Graziele Pereira Bezerra
et al.
Abstract:We evaluated the influence of different media plus various concentrations of Glial cell line‐derived neurotrophic factor (GDNF) during the in vitro culture (IVC) of testicular tissues from prepubertal collared peccary. Testes from 5 individuals were collected, fragmented and cultured for 28 days (34°C and 5% CO2). Culture media were Dulbecco's modified essential medium (DMEM) or stem cell serum free media (StemPro‐34™ SFM), both supplemented with various concentrations of GDNF (0, 10, or 20 ng/mL). Fragments w… Show more
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