Synergistic effects of fibronectin and bone morphogenetic protein on the bioactivity of titanium metal

Abstract

To improve the biological properties of bioactive titanium metal, recombinant human bone morphogenetic protein 2(rhBMP-2) and fibronectin (Fn) were adsorbed on its surface solely or contiguously to modify the anodic oxidized titanium (AO-Ti), acid-alkali-treated titanium (AA-Ti), and polished titanium (P-Ti). It is found that the different bioactive titanium surface structures had great influence on protein adsorption. The adsorption amounts of BMP adsorbed solely and Fn/BMP adsorbed contiguously were AA-Ti > P-Ti > AO-Ti, and that for Fn adsorbed solely was AA-Ti ≈ P-Ti > AO-Ti. The conformation of proteins was changed remarkably after the adsorption. For BMP, the α-helix decreased on AA-Ti and stabilized on P-Ti and AO-Ti. For Fn, the β-sheet on PT-Ti and AA-Ti increased significantly. For Fn/BMP, the percentage of β-sheet on AA-Ti increased, and that of α-helix on all samples was stable. MSCs showed greater adhesion and spreading on Fn/BMP groups. MTT and Elisa tests showed that the synergistic effects of proteins made the cells proliferate and differentiate faster. It indicated both the surface structure and the synergistic effects of proteins could influence the biological properties of titanium metals. It provides research foundation for improving the biological properties of bioactive titanium metals by simultaneous application of several proteins. © 2017 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 105A: 2485-2498, 2017.

Protein adsorption and bioactivity of functionalized electrospun membranes for bone regeneration

“…Fn is an abundant adhesion protein found in serum and in many extracellular matrices. Fn intermediates in cell adhesion and signalling [23]. Similar Fn adsorption was found on both experimental membranes, and it was high above the adsorption values encountered for PTFE.…”

The progress on physicochemical properties and biocompatibility of tantalum-based metal bone implants

“…Therefore, Ta coating and Ta 2 O 5 thin film are the main forms of research on Ta in vitro. For Ta, topographic surface design, especially nanoscale chemistry and morphology, is conducive to protein adsorption, which will promote cell proliferation and differentiation, especially the enhancement of bone regeneration ability [77,78]. Quartz crystal microbalance (QCM-D) is a unique technique to measure the adsorption capacity and viscoelasticity of proteins on the metal surface, which can be used to infer the orientation and conformation changes of adsorbed proteins [79,80].…”

Antimicrobial and Osseointegration Properties of Nanostructured Titanium Orthopaedic Implants

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