Synergistic Effects of Exercise Training and Vitamin D Supplementation on Mitochondrial Function of Cardiac Tissue, Antioxidant Capacity, and Tumor Growth in Breast Cancer in Bearing-4T1 Mice

Jafari, Alireza; Sheikholeslami-Vatani, Dariush; Khosrobakhsh, Farnoosh; Khaledi, Neda

doi:10.3389/fphys.2021.640237

Cited by 10 publications

(7 citation statements)

References 53 publications

(65 reference statements)

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“…The addition of vitamin D to human skeletal muscle cells was reported to enhance skeletal-muscle mitochondrial fusion and inhibit mitochondrial fission (12). In addition, in cardiac tissue in mice, vitamin D treatment significantly enhanced the mRNA expressions of peroxisome proliferator-activated receptor-gamma coactivator (PGC)-1a (a marker of mitochondrial biogenesis) and dynamin-related protein (DRP)1 (a marker of mitochondrial fission) (13). Although the effects of vitamin D administration on skeletal muscle have been reported, the effects of vitamin D deficiency on skeletal-muscle mitochondrial biogenesis, dynamics, and quality control have not been clarified.…”

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confidence: 99%

Effects of Dietary Vitamin D Deficiency on Markers of Skeletal Muscle Mitochondrial Biogenesis and Dynamics

NAKAGAWA

TOMIGA

et al. 2022

J Nutr Sci Vitaminol

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We examined the effects of dietary vitamin D deficiency on markers of mitochondrial biogenesis and dynamics in rat soleus muscle. Male Wistar rats were fed a chow with no vitamin D (No-D; 0 IU/kg) or a moderate dose (Mod-D; 2,000 IU/kg) of vitamin D chow for 8 wk. Compared to the Mod-D group, at 8 wk the No-D group showed significantly lower serum 25(OH)D levels. Although vitamin D deficiency had no effect on body composition, the No-D rats showed significantly decreased levels of PGC-1a, a marker of skeletal muscle mitochondrial biogenesis, and DRP1, a marker of skeletal muscle mitochondrial fission. The change in the PGC-1a protein expression and the serum 25(OH)D concentrations were significantly correlated. The change in DRP1 protein expression and the serum 25(OH)D concentrations tended to be correlated. There was no significant between-group difference in markers of mitochondrial fusion (MFN2 and OPA1) and mitophagy (PARKIN) in soleus muscle, and no relationship with serum 25(OH)D concentrations. Collectively our findings suggest that dietary vitamin D deficiency decreased PGC-1a and DRP1 protein expression in rat soleus muscle.

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confidence: 99%

Effects of Dietary Vitamin D Deficiency on Markers of Skeletal Muscle Mitochondrial Biogenesis and Dynamics

NAKAGAWA

TOMIGA

et al. 2022

J Nutr Sci Vitaminol

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“…A further notable result in the current study was that ingestion of sturgeon fillets increased the amount of intake in food and the blood concentration of 25OHD. Antioxidant and anti‐inflammatory effects of vitamin D have been shown in previous reports 29–38 . Vitamin D deficiency is defined as 25OHD <20 ng/mL (50 nmol/L) and vitamin D insufficiency as 25OHD of 21–29 ng/mL (525–725 nmol/L) 39 .…”

Section: Discussionmentioning

confidence: 93%

“…Antioxidant and anti-inflammatory effects of vitamin D have been shown in previous reports. [29][30][31][32][33][34][35][36][37][38] Vitamin D deficiency is defined as 25OHD <20 ng/mL (50 nmol/L) and vitamin D insufficiency as 25OHD of 21-29 ng/mL (525-725 nmol/L). 39 However, seven of the nine runners had a blood 25OHD level of 29 ng/mL or less.…”

Section: Discussionmentioning

confidence: 99%

Effects of sturgeon fillet intake on top‐ranked Japanese female long‐distance runners

Haraguchi

Nakao²,

Sakakibara

et al. 2023

J of Obstet and Gynaecol

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Aims The aim of this study is to investigate whether consumption of sturgeon fillets reduces the oxidative stress marker urinary 8‐hydroxy‐2′‐deoxyguanosine (8OHdG) in top‐ranked Japanese female long‐distance runners. Methods In a before‐and‐after study, nine professional long‐distance female athletes ate 100 g/day of sturgeon fillets for 2 weeks. Urinalysis (8OHdG, an oxidative stress marker, and creatinine), blood tests (fatty acids and 25‐hydroxyvitamin D [25OHD]), exercise intensity, subjective fatigue, muscle elasticity, muscle mass, body fat mass, and nutritional intake using image‐based dietary assessment (IBDA) were compared before, immediately after, and 1 month after the intervention. Results Consumption of sturgeon fillets suppressed 8OHdG (p < 0.05) in the increased exercise intensity female athletes. Eicosapentaenoic acid (EPA), docosahexaenoic acid (DHA), and 25OHD levels in blood increased from before to immediately after and 1 month after the intervention (p < 0.05). IBDA showed that intake of n‐3 fatty acid increased after and one month after the intervention, whereas DHA, imidazole dipeptide and vitamin D intake increased after the intervention (p < 0.05) and then decreased after 1 month (p < 0.05). There were no significant changes in subjective fatigue, muscle elasticity, muscle mass, and body fat. Conclusions The results suggest that eating sturgeon fillets during intense training may increase blood levels of EPA, DHA, and 25OHD, which may suppress urinary oxidative stress (8OHdG) in top‐ranked Japanese long‐distance runners.

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“…Moreover, the MAPK1 signaling pathway has known to be crucial in the transcription and translation of biomarkers of inflammation and oxidative stress [ 69 , 70 ]. Jafari et al (2021) reported that Vit D supplementation along with exercise modulates antioxidant defense and the expression of antioxidant genes [ 71 ]. Vit D is also considered an antioxidant that might cause the expression of several genes involved in the antioxidant defense system, including GPX, GSH, CAT, SOD, and the suppression of NADPH oxidase [ 72 , 73 ].…”

Section: Discussionmentioning

confidence: 99%

Concurrent alteration in inflammatory biomarker gene expression and oxidative stress: how aerobic training and vitamin D improve T2DM

Hoseini

Rahim

Ahmed

2022

BMC Complement Med Ther

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Background Vitamin D (Vit D) supplementation and Aerobic Training (AT) exert several beneficial effects such as antioxidant and anti-inflammatory actions. The literature on the effects of AT and Vit D supplementation on the oxidative stress biomarkers and gene expression of inflammatory cytokines in patients with Type 2 Diabetes Mellitus (T2DM) is limited. The present study aimed to examine the effects of AT and Vit D supplementation on inflammation and oxidative stress signaling pathways in T2DM patients. Materials and methods In this single-blinded, randomized, placebo-controlled trial, 48 men with T2DM (aged 35–50 years with Body Mass Index (BMI) of 25–30 kg/m2) were randomly allocated into four groups: AT+Vit D (n = 10); AT + placebo (AT; n = 10); Vit D (n = 10), and Control + placebo (C; n = 10). The eight-week AT program was executed for 20–40 min/day, at 60–75% of heart rate maximum (HRmax), for 3 days/wks. The Vit D group received 50,000 IU of Vit D supplement capsules per week for 8 weeks. The serum levels of oxidative stress biomarkers and gene expression of inflammatory cytokines in the Peripheral Blood Mononuclear Cells (PBMCs) were evaluated using the RT-PCR method. To analyze the data, paired t-tests and one-way analysis of variance and Tukey’s post hoc test were used at the significance level of P < 0.05. Results The result shows that serum 25-OH-Vit D, total nitrite, Total Glutathione (GSH), Total Antioxidant Capacity (TAC), Superoxide Dismutase (SOD), Catalase (CAT), and Glutathione Peroxidase (GPX) increased; and insulin, Fasting Blood Glucose (FBG), Homeostasis Model Assessment of Insulin Resistance (HOMA-IR), High Sensitivity C-Reactive Protein (hs-CRP), Malondialdehyde (MDA), glycated albumin, and Urinary 8-hydroxydeoxyguanine (8-OHdG) decreased significantly in all groups after 8 weeks, except for C. In addition, results of RT-PCR showed that AT+Vit D, Vit D, and AT significantly downregulated the gene expression of Tumor Necrosis Factor-Alpha (TNF-α), Interleukin-1 Beta (IL-1β), Mitogen-Activated Protein Kinases 1 (MAPK1), Nuclear Factor Kappa B (NF-κB) 1 (p50). It also upregulated Interleukin-4 (IL-4) gene expression, Peroxisome Proliferator-Activated Receptor Gamma (PPAR-γ) in T2DM patients compared to the C. Conclusion Additionally, the AT+Vit D group showed significantly lower insulin, FBG, HOMA-IR, hs-CRP, MDA, glycated albumin, urinary 8-OHdG, IL-1β, TNF-α, MAPK1, and NF-κB1 (p50) levels and significantly higher serum 25-OH-Vit D, total nitrite, GSH, TAC, CAT, SOD, GPX, IL-4, and PPAR-γ levels compared to the AT and Vit D groups. In T2DM patients, 8 weeks of AT+Vit D had a more significant impact on certain gene expressions related to inflammation and oxidative stress than Vit D or AT alone.

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Synergistic Effects of Exercise Training and Vitamin D Supplementation on Mitochondrial Function of Cardiac Tissue, Antioxidant Capacity, and Tumor Growth in Breast Cancer in Bearing-4T1 Mice

Cited by 10 publications

References 53 publications

Effects of Dietary Vitamin D Deficiency on Markers of Skeletal Muscle Mitochondrial Biogenesis and Dynamics

Effects of Dietary Vitamin D Deficiency on Markers of Skeletal Muscle Mitochondrial Biogenesis and Dynamics

Effects of sturgeon fillet intake on top‐ranked Japanese female long‐distance runners

Concurrent alteration in inflammatory biomarker gene expression and oxidative stress: how aerobic training and vitamin D improve T2DM

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