Synergistic effects of dietary supplementation of Bacillus subtilis WB60 and mannanoligosaccharide (MOS) on growth performance, immunity and disease resistance in Japanese eel, Anguilla japonica

Lee, Seunghan; Katya, Kumar; Hamidoghli, Ali; Hong, Jeongwhui; Kim, Dae Jung; Bai, Sungchul C.

doi:10.1016/j.fsi.2018.09.031

Cited by 62 publications

(63 citation statements)

References 44 publications

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“…In the present study, significant enhancement in lysozyme activity was found at BS levels higher than 3 × 10 7 CFU/g feed. This is in agreement with results from rohu (Labeo rohita; Giri, Sukumaran, Sen, & Jena, 2014;Ramesh & Souissi, 2018), Japanese eel (Anguilla japonica; Lee et al, 2018), hybrid Hulong grouper (Zhou et al, 2019), and Nile tilapia (Oreochromis niloticus; Galagarza et al, 2018). It has been known that the innate immunity of fish can be enhanced by interactions between probiotics and intestinal epithelial cells.…”

Section: Discussionsupporting

confidence: 90%

The effects of dietary Bacillus subtilis on immune response, hematological parameters, growth performance, and resistance of juvenile olive flounder (Paralichthys olivaceus) against Streptococcus iniae

Lee

Cha

Kim

et al. 2019

J World Aquaculture Soc

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Section: Discussionsupporting

confidence: 90%

The effects of dietary Bacillus subtilis on immune response, hematological parameters, growth performance, and resistance of juvenile olive flounder (Paralichthys olivaceus) against Streptococcus iniae

Lee

Cha

Kim

et al. 2019

J World Aquaculture Soc

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“…Hamidoghli et al, ). However, there was only one study on the synergistic effects of dietary probiotics and prebiotics (synbiotics) in this species (Lee et al, ). The previous study proved that combination of B. subtilis WB60 at 0.5 × 10 7 CFU/g and manna oligosaccharide at 5 g/kg diet could have beneficial synergistic effects on growth performance, non‐specific immune responses, intestinal morphology and disease resistance in Japanese eel.…”

Section: Introductionmentioning

confidence: 99%

Effects of two dietary probiotics ( Bacillus subtilis or licheniformis ) with two prebiotics (mannan or fructo oligosaccharide) in Japanese eel, Anguilla japonica

et al. 2019

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A 12‐week feeding trial was conducted to investigate the effects of two dietary probiotics; Bacillus subtilis KCTC 2217 or Bacillus licheniformis KCCM 11775 with two prebiotics; mannan oligosaccharide (MOS) or fructooligosaccharide (FOS) in Japanese eel. Fish averaging 12.8 ± 0.47 g (mean ± SD) were randomly distributed into five treatments with triplicate tanks. A basal control diet (CON) and four synbiotic diets supplementing B. subtilis + MOS (BSM), B. subtilis + FOS (BSF), B. licheniformis + MOS (BLM), and B. licheniformis + FOS (BLF). Weight gain and specific growth rate of fish fed all synbiotic diets were higher than those of fish fed CON. Immune‐related gene expression of heat shock protein 70 and immunoglobulin M of fish fed BSF and BLM were significantly higher than those of fish fed CON. Fish fed BSF and BLM had significantly higher intestinal villi length than those of fish fed BLF and CON (p < .05). Disease resistance against Aeromonas hydrophila of fish fed all synbiotic diets were significantly higher than those of fish fed CON (p < .05). Therefore, these results indicated that dietary B. subtilis with FOS (BSF) and B. licheniformis with MOS (BLM) could have beneficial effects on intestinal morphology, and immune‐related gene expression in Japanese eel.

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“…On the other hand, WPC inclusion in fish diets beside probiotic L. Plantarum (T4–T6) improved the antioxidant activity of Nile tilapia over those fed the individual supplement alone. In similar study, Lee et al () found that Japanese eel fed B. subtilis and/or MOS‐supplemented diet exhibited a higher superoxide dismutase (SOD) activity than the control group. They also clearly found that probiotic B. subtilis , regardless of the dosage and presence/absence of prebiotics in the diet, could stimulate the SOD activity over the control diet.…”

Section: Discussionmentioning

confidence: 90%

“…This finding may be due to the fact that WPC has many bioactive compounds including polysaccharides, which were used as matrices for bacterial growth. In this respect, Lee et al () fed Bacillus subtilis on different prebiotics for different incubation periods and they found that the bacterial viability and growth was recorded to be peaked with prebiotic, β‐1,3 glucan and mannonoligosaccharide (MOS) after 15 hr of incubation.…”

Section: Discussionmentioning

confidence: 99%

Effects of dietary probioticLactobacillus plantarumand whey protein concentrate on the productive parameters, immunity response and susceptibility of Nile tilapia,Oreochromis niloticus(L.), toAeromonas sobriainfection

AbouElatta¹,

Abdel‐Tawwab

Abdel‐Razek³

et al. 2019

Aquacult Nutr

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Immunostimulatory feed supplements have an increasingly interest in aquaculture management. Generally, an individual supplement was used in fish diets but it is expected that the use of multi-supplements may show synergistic enhancements in fish performance, health, and immunity. Therefore, the present investigation was carried out to evaluate the use of dietary probiotic Lactobacillus plantarum and whey protein concentrate (WPC) in practical diets for Nile tilapia, Oreochromis niloticus. Hence, probiotic L. plantarum, WPC and their mixture were incorporated into a basal fish diet (300 g/kg crude protein) as follows: T1 = a basal control diet, T2 = a basal diet containing L. plantarum, T3 = a basal diet containing 1.0 g WCP/kg diet and T4, T5 or T6 = basal diets containing probiotic L. plantarum + 1.0, 2.0 or 3.0 g WCP/kg diet, respectively. Fish (15.2 ± 0.6 g) were fed on one of the tested diets up to apparent satiation twice a day for 60 days. After that, fish were intraperitoneally injected with pathogenic bacteria Aeromonas sobria and fish mortality was observed for 10 days postchallenge. Fish growth and feed intake were significantly improved by dietary probiotic L. plantarum (T2) and/or WPC (T3) over the control group (T1), and highest fish performance was observed in T5-T6 fish groups. Similarly, highest values of haematocrit, glucose, total proteins, albumin, and globulin were significantly observed in T5-T6 fish groups. Likewise, fish fed dietary probiotic L. plantarum (T2), WPC (T3), and their mixture (T4-T6) showed antioxidants and immune-stimulating activities better than the control group. Fish fed the control diet were more susceptible to A. sobria infection showing highest fish mortality (75.0%). Meanwhile, dietary probiotic L. plantarum (T2), WPC (T3), and their mixture (T4-T6) enhanced significantly the fish resistance to A. sobria infection resulting in maximum values of relative percent of fish survival (73.3%-80.0%) in T5-T6 groups. The present investigation recommended the use of probiotic L. plantarum with 2.0 g WPC/kg diet to improve the growth, antioxidant, immunity responses and tolerance of Nile tilapia to A. sobria infection. Fresh whey was collected from the nearby village of the Central Laboratory for Aquaculture Research (CLAR), Abbassa, Abo-Hammad, Egypt, and transferred immediately to the Microbiology Lab, CLAR. All samples were analysed on the same day of sampling and kept at refrigerated (2-8°C) condition until doing the microbiological analysis. A 10 ml of fresh liquid whey was homogenized with 90 ml of 0.85% (w/v) sterile sodium chloride solution to make serial dilutions up to 10 −6 for each sample. A 1.0 ml sample from each of the corresponding dilutions (10 −5 and 10 −6 ) were inoculated into De Man, Rogosa and Sharpe agar (MRS agar; Merck, Germany) for lactobacilli isolation (De Man et al., 1960) and incubated at 30°C for 24-48 hr. After incubation, plates with 30-300 colonies were enumerated and the calculated results were expressed as colonyforming unit (CFU) per ml by mul...

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Synergistic effects of dietary supplementation of Bacillus subtilis WB60 and mannanoligosaccharide (MOS) on growth performance, immunity and disease resistance in Japanese eel, Anguilla japonica

Cited by 62 publications

References 44 publications

The effects of dietary Bacillus subtilis on immune response, hematological parameters, growth performance, and resistance of juvenile olive flounder (Paralichthys olivaceus) against Streptococcus iniae

The effects of dietary Bacillus subtilis on immune response, hematological parameters, growth performance, and resistance of juvenile olive flounder (Paralichthys olivaceus) against Streptococcus iniae

Effects of two dietary probiotics ( Bacillus subtilis or licheniformis ) with two prebiotics (mannan or fructo oligosaccharide) in Japanese eel, Anguilla japonica

Effects of dietary probioticLactobacillus plantarumand whey protein concentrate on the productive parameters, immunity response and susceptibility of Nile tilapia,Oreochromis niloticus(L.), toAeromonas sobriainfection

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