Synergistic effects of BAP and kinetin media additives on regeneration of vetiver grass (Vetiveria zizanioides L. Nash)

Abstract

Regeneration efficiency was enhanced by adding auxin and cytokinin at different concentrations to the media. It was found that the two highest frequencies-90 and 85%-of callus induction occured when the buds were cultured in an MS medium supplemented with 2 mgL-1 α-naphthalene acetic acid (NAA) plus 1 mgL-1 N 6-benzylaminopurine (BAP) (CIM2) and in the same medium supplemented with 2 mgL-1 NAA plus 1 mgL-1 kinetin (CIM5), respectively. When cultured further in a plant regeneration medium supplemented with 1 mgL-1 NAA and 2 mgL-1 BAP (PRM1), these calli regenerated with high efficiencies, 80% for calli grown in CIM2 and 85% for calli grown in CIM5. The low BAP concentration in CIM1 (2 mgL-1 NAA and 0.5 mgL-1 BAP) resulted in a low regeneration efficiency and fewer shoots per callus even though the PRM3 regeneration medium had a high concentration of cytokinins (2 mgL-1 BAP and 2 mgL-1 kinetin). However, the calli cultured in CIM2 without kinetin but with a suitable level of BAP still regenerated with high efficiencies in PRM2 and PRM3 that contained 1 and 2 mgL-1 kinetin, respectively. Similarly, when calli cultured in CIM5 without BAP but with kinetin were regenerated in PRM1 with BAP alone, they also regenerated with a high regeneration efficiency. These results showed that culturing a specific tissue in a medium with suitable concentrations of NAA and BAP or kinetin resulted in a large number of multiple shoots, and BAP and kinetin worked synergistically to produce a high plant regeneration efficiency.

High frequency plant regeneration of Chrysopogon zizanioides via organogenesis and somatic embryogenesis-an underutilized pharmaceutically valuable biofuel plant

“…The compact friable calli obtained from culturing of leaf and root explants were transferred onto shoot initiation medium augmented with varied levels and mixtures of BAP and NAA. It has been found that both the explants exhibited the highest frequency of shoot redevelopment on MS by NAA of 1.0 mg L -1 and BAP of 1.0 mg L -1 as stated in V. zizanioides (Sompornpailin et al 2016;Sompornpailin 2009). Also the Zinc Oxide Nanoparticles showed some impact on calli derived regenerants in V. zizanioides (Khunchuay et al 2017).…”

In vitro establishment and multiplication of Echynochloa polystachya

“…Thus, they verified successes in embryogenic regenerations and future genetic transformations in this species. Similarly, Sompornpailin and Khunchuay (2016) were successful in regenerating calluses from roots in "vertiver grass" (Vetiveria zizanioides (L.) Nash) between 85% to 90% using the average concentration of 2.0 mg L -1 and 20 g L -1 sucrose associated with another phytoregulator. Silva et al (2017) tested lower concentrations of BAP (0.0; 0.25; 0.5; 1.0; 1.5 mg L -1 ) in MS medium plus of 30 g L -1 sucrose and verify that despite these concentrations not having been significantly influenced, provided higher survival rate and shoots in the nodal segments of Rosa sp.…”

Multiplikasi Tunas in vitro Sukun Bone (Artocarpus altilis (Park.) Fosberg) pada Media WP dengan Penambahan Sitokinin dan Adenin Sulfat

“…Jumlah tunas aksiler yang terbentuk merupakan parameter yang sangat penting dalam perbanyakan in vitro secara massal, oleh karena itu media terbaik untuk multiplikasi kultur tunas sukun Bone adalah media BAK. Efek sinergitas antara BAP dan Kinetin untuk menghasilkan multiplikasi tunas yang tinggi juga dilaporkan oleh Shyamali (2007) dan Sompornpailin (2016). Salah satu hal yang cukup menarik pada percobaan ini adalah respon tunas pada media BAA seperti yang tersaji pada tabel 1 dan pada gambar 5.…”