Synergistic Effects of an Atmospheric-Pressure Plasma Jet and Pulsed Electric Field on Cells and Skin

Jiang, Chunqi; Oshin, Edwin; Guo, Siqi; Scott, Megan; Xi, Li; Mangiamele, Cathryn; Heller, Richard

doi:10.1109/tps.2021.3113260

Cited by 12 publications

(11 citation statements)

References 71 publications

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“…The transdermal diffusion of RONS across pig skin has been observed under specific operational conditions, such as discharge frequency [ 111 , 112 ]. A recent study found that a 300-ns 50-kV/cm pulsed electric field increased the transdermal diffusion of RONS across a pig skin model [ 113 ]. Altogether, these preliminary investigations suggest that the transdermal diffusion of reactive species underneath a millimeter-level thick tissue-mimic film is possible after CAP treatment.…”

Section: Mechanism Discussionmentioning

confidence: 99%

Preclinical Cold Atmospheric Plasma Cancer Treatment

Limanowski

Yan

Lin

et al. 2022

Cancers

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CAP is an ionized gas generated under atmospheric pressure conditions. Due to its reactive chemical components and near-room temperature nature, CAP has promising applications in diverse branches of medicine, including microorganism sterilization, biofilm inactivation, wound healing, and cancer therapy. Currently, hundreds of in vitro demonstrations of CAP-based cancer treatments have been reported. However, preclinical studies, particularly in vivo studies, are pivotal to achieving a final clinical application. Here, we comprehensively introduced the research status of the preclinical usage of CAP in cancer treatment, by primarily focusing on the in vivo studies over the past decade. We summarized the primary research strategies in preclinical and clinical studies, including transdermal CAP treatment, post-surgical CAP treatment, CAP-activated solutions treatment, and sensitization treatment to drugs. Finally, the underlying mechanism was discussed based on the latest understanding.

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Section: Mechanism Discussionmentioning

confidence: 99%

Preclinical Cold Atmospheric Plasma Cancer Treatment

Limanowski

Yan

Lin

et al. 2022

Cancers

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“…It was also found that plasma-treated PBS reduces the PEF amplitude required for cell membrane electropermeabilization [ 45 ]. Using direct plasma treatment, one study found increased inactivation of pancreatic cancer cells and an augmented efficiency of gene electrotransfer by combining PEF in the nanosecond range and nanosecond pulsed plasma jets [ 46 ]. Hence, the experimental approach can be modulated so that the electroporation or ROS entrance into the cell is facilitated by plasma pretreatment.…”

Section: Discussionmentioning

confidence: 99%

Combined In Vitro Toxicity and Immunogenicity of Cold Plasma and Pulsed Electric Fields

2022

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In modern oncology, therapies are based on combining monotherapies to overcome treatment resistance and increase therapy precision. The application of microsecond-pulsed electric fields (PEF) is approved to enhance local chemotherapeutic drug uptake within combination electrochemotherapy regimens. Reactive oxygen species (ROS) have been implicated in anticancer effects, and cold physical plasma produces vast amounts of ROS, which have recently been shown to benefit head and neck cancer patients. PEF and cold plasma technology have been linked to immunogenic cell death (ICD) induction, a regulated cell death accompanied by sterile inflammation that promotes antitumor immunity. To this end, we investigated the combined effect of both treatments regarding their intracellular ROS accumulation, toxicity, ICD-related marker expression, and optimal exposure sequence in a leukemia model cell line. The combination treatment substantially increased ROS and intracellular glutathione levels, leading to additive cytotoxic effects accompanied by a significantly increased expression of ICD markers, such as the eat-me signal calreticulin (CRT). Preconditioned treatment with cold plasma followed by PEF exposure was the most potent treatment sequence. The results indicate additive effects of cold plasma and PEF, motivating further studies in skin and breast tumor models for the future improvement of ECT in such patients.

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“…Over the past few years, the application potential of atmospheric pressure plasma jet (APPJ) has attracted high attention in material surface modification [1][2][3][4], disinfection and sterilization [5][6][7][8], medical treatment [9][10][11][12][13][14][15][16][17], diagnosis APPJ simulation published by our research group studied the propagation mechanism of He APPJ impinging on a dielectric material surface [21]. In recent years, the influence of addition gases such as O 2 , N 2 , H 2 O, CF 4 and C 4 F 8 on physical and chemical characteristics, reactive species composition, influence on surface modification and other applications of APPJ have been gradually studied by researchers.…”

Section: Introductionmentioning

confidence: 99%

Effect of CF₄ concentration on the discharge dynamics and reactive species distribution of atmospheric nanosecond pulsed He plasma jet

Zhao,

Wang,

Liu

2023

J. Phys. D: Appl. Phys.

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Based on a two dimensional (2D) self-consistent model, the effect of different CF4 concentrations on the discharge dynamics characteristics, F-containing species distribution and the distribution of the axial and radial ionization rates of He atmospheric pressure plasma jet (APPJ) has been investigated. In addition, the accuracy of the simulation is verified by comparing the experimental results. The ionization rate of He APPJ enhances with the increase of CF4 from 0 to 2% because of the Penning ionization of CF4 with metastable He while decreases in the case of 3% CF4 owing to the electronegativity and the electron excitation energy loss of CF4. The increase of CF4 concentration and the decrease of the electron density leads to the decrease of CF4+ density at 3%CF4. CF3+ density is determined by the Penning ionization rate of CF4 and the reaction rate of He+ + CF4 → CF3+ + F + He. As CF4 concentration increases from 2% to 3%, the decrease of metastable He and He+ concentration results in the significant decrease of CF3+ density. The densities of CF3⁻ and F⁻ keep increasing from 1% to 3%CF4 due to the effect of the electron attachment reaction of CF4. With the addition of CF4, the ionization reaction of CF4 is gradually playing the major role in the streamer head, the Penning ionization of CF4 is the main source of electron ionization inside the jet and dominates in the formation of ionization wave tail. As the concentration of CF4 increases from 0 to 2%, the electron impact ionization rates of He and CF4 near the axis increase, resulting in the more uniform radial ionization rate distribution of He + 2%CF4 APPJ.

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Synergistic Effects of an Atmospheric-Pressure Plasma Jet and Pulsed Electric Field on Cells and Skin

Cited by 12 publications

References 71 publications

Preclinical Cold Atmospheric Plasma Cancer Treatment

Preclinical Cold Atmospheric Plasma Cancer Treatment

Combined In Vitro Toxicity and Immunogenicity of Cold Plasma and Pulsed Electric Fields

Effect of CF₄ concentration on the discharge dynamics and reactive species distribution of atmospheric nanosecond pulsed He plasma jet

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Synergistic Effects of an Atmospheric-Pressure Plasma Jet and Pulsed Electric Field on Cells and Skin

Cited by 12 publications

References 71 publications

Preclinical Cold Atmospheric Plasma Cancer Treatment

Preclinical Cold Atmospheric Plasma Cancer Treatment

Combined In Vitro Toxicity and Immunogenicity of Cold Plasma and Pulsed Electric Fields

Effect of CF4 concentration on the discharge dynamics and reactive species distribution of atmospheric nanosecond pulsed He plasma jet

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Product

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Effect of CF₄ concentration on the discharge dynamics and reactive species distribution of atmospheric nanosecond pulsed He plasma jet