Synergistic effects between silver nanoparticles and antibiotics and the mechanisms involved

Hwang, In-sok; Hwang, Jun Hyun; Choi, Heejung; Kim, Keuk‐Jun; Lee, Dong Gun

doi:10.1099/jmm.0.047100-0

Cited by 231 publications

(159 citation statements)

References 36 publications

Supporting

Mentioning

146

Contrasting

Order By: Relevance

“…Recently, several studies have indicated that AgNPs may strengthen the antibacterial effects of antibiotics against both susceptible and resistant bacteria, either additively or synergistically (Fayaz et al, 2010;Lara et al, 2010;Hwang et al, 2012). Consistent with these findings, combining two agents with reported multimodal antibacterial action, in the present study, has shown at least additive or synergistic effect for all tested strains (Table 2).…”

Section: Discussionsupporting

confidence: 80%

“…Silver nanoparticles were shown to inactivate bacterial enzymes, disrupt bacterial metabolic processes, increase the cytoplasmic membrane permeability, interact with DNA and generate reactive oxygen species that damage biomacromolecules (Lara et al, 2010;Franci et al, 2015). The studies reporting the different mechanisms by which AgNPs interact with microbes paralleled with several reports that investigated the synergistic potential of AgNPs with classical antibiotics (Fayaz et al, 2010;Hwang et al, 2012;Naqvi et al, 2013;Singh et al, 2013). In these studies, quantifying the synergistic effects of antibiotics in combination with AgNPs against an assortment of microorganisms has been investigated, employing the microdilution method.…”

Section: Discussionmentioning

confidence: 85%

See 1 more Smart Citation

Combined efficacy of thymol and silver nanoparticles against Staphylococcus aureus

Abdelhamid¹,

El‐Hosseiny²

2017

Afr. J. Microbiol. Res.

View full text Add to dashboard Cite

Due to the looming spread of resistance to classical antimicrobial agents, innovative therapeutic methods are in dire need to combat the onslaught of resistant bacterial pathogens. This study examines the antimicrobial efficacy of a phytochemical and a metallic nanoparticle against the top Gram positive resistant pathogen. The potential synergy of these two agents was also evaluated. The antibacterial activity of thymol and silver nanoparticles were tested individually using disc diffusion technique. The extent of synergy of their combination was evaluated using the checkerboard assay. Twenty clinical isolates of Staphylococcus aureus characterized as methicillin resistant or methicillin sensitive Staphylococcus aureus were utilized and the extent of synergism was calculated from fractional inhibitory concentration indices. Thymol exhibited an antistaphylococcal activity regardless of whether the isolates were phenotypically resistant or sensitive to methicillin. Combining thymol with silver nanoparticles resulted in at least additive or synergistic effect for all the examined strains and methicillin resistant strains were inhibited in the combinatorial assays to a greater extent comparative to when silver nanoparticles or thymol were used singly.

show abstract

Section: Discussionsupporting

confidence: 80%

Section: Discussionmentioning

confidence: 85%

Combined efficacy of thymol and silver nanoparticles against Staphylococcus aureus

Abdelhamid¹,

El‐Hosseiny²

2017

Afr. J. Microbiol. Res.

View full text Add to dashboard Cite

show abstract

“…45,47 Previous studies have reported enhanced outcomes with a combination of Ag nanoparticles and antibiotics in vitro. [27][28][29][30][31] However, the synergistic antibacterial activity is rarely tested in vivo. In this regard, we built an implant infection model with MRSA (ATCC 43300) and treated it with the combinational antibacterial therapy for further verification of the in vitro results.…”

Section: Discussionmentioning

confidence: 99%

“…25,26 Several recent studies indicated that Ag nanoparticles could enhance the antibacterial effect of antibiotics, such as vancomycin, gentamicin, rifampicin, and levofloxacin. [27][28][29][30][31] The mechanism of the synergistic effect is complicated and varies with the type of antibiotics. For example, the destruction of cell wall by Ag nanoparticles or ions makes the antibiotic-resistant bacteria more sensitive to antibiotics.…”

Section: Introductionmentioning

confidence: 99%

Silver-loaded nanotubular structures enhanced bactericidal efficiency of antibiotics with synergistic effect in vitro and in vivo

Cheng

et al. 2017

IJN

View full text Add to dashboard Cite

Antibiotic-resistant bacteria have become a major issue due to the long-term use and abuse of antibiotics in treatments in clinics. The combination therapy of antibiotics and silver (Ag) nanoparticles is an effective way of both enhancing the antibacterial effect and decreasing the usage of antibiotics. Although the method has been proved to be effective in vitro, no in vivo tests have been carried out at present. Herein, we described a combination therapy of local delivery of Ag and systemic antibiotics treatment in vitro in an infection model of rat. Ag nanoparticle-loaded TiO 2 nanotube (NT) arrays (Ag-NTs) were fabricated on titanium implants for a customized release of Ag ion. The antibacterial properties of silver combined with antibiotics vancomycin, rifampin, gentamicin, and levofloxacin, respectively, were tested in vitro by minimum inhibitory concentration (MIC) assay, disk diffusion assay, and antibiofilm formation test. Enhanced antibacterial activity of combination therapy was observed for all the chosen bacterial strains, including gram-negative Escherichia coli (ATCC 25922), gram-positive Staphylococcus aureus (ATCC 25923), and methicillin-resistant Staphylococcus aureus (MRSA; ATCC 33591 and ATCC 43300). Moreover, after a relative short (3 weeks) combinational treatment, animal experiments in vivo further proved the synergistic antibacterial effect by X-ray and histological and immunohistochemical analyses. These results demonstrated that the combination of Ag nanoparticles and antibiotics significantly enhanced the antibacterial effect both in vitro and in vivo through the synergistic effect. The strategy is promising for clinical application to reduce the usage of antibiotics and shorten the administration time of implant-associated infection.

show abstract

“…Thereby, Ag-NPs, unlike antibiotics, can easily pass cellular membrane. It can be concluded that silver nanoparticles bonded antibiotics can be easily delivered to the cell (Hwang et al, 2012).…”

Section: Antibacterial Activitymentioning

confidence: 99%

Biosynthesis of Silver Nanoparticles Using Ganoderma Lucidum and Assessment of Antioxidant and Antibacterial Activity

Poudel¹,

Pokharel²,

Sudip³

et al. 2017

Int J Appl Sci Biotechnol

View full text Add to dashboard Cite

There is an increasing interest these days in the green route of synthesis of metal nanoparticles using plant extract, fungus and bacterial-mediated eco-friendly materials. Silver nanoparticles were synthesized using an intracellular extract of Ganoderma lucidum, a mushroom from Nepal then characterization of silver nanoparticles was performed. The Ag-NPs thus formed show surface plasmonic resonance with a maximum absorption band at 420 nm. Their crystalline nature was confirmed as a facecentered cubic structure by the XRD, Furthermore, SEM revealed that they were in the size range of 10-30 nm and were spherical in shape. The possible biomolecule involved in the reduction and stabilization of Ag-NPs were believed to be oxidized polyphenol, and carbonyl group, amino acid residue. Ag-NPs exhibit good antioxidant activity but showed low antioxidant in comparison to the fungal extract alone, which was studied using DPPH antioxidant assay. The effect of the colloidal silver nanoparticles solution against six human pathological bacteria was carried out by Disc diffusion method. The zone of maximum inhibition was seen in Bacillus subtilis (17.0 ± 0.13 mm) and least effective against Escherichia coli (10.1 ± 0.2 mm). Further, the results showed that Ag-NPs in combination with antibiotics have better antibacterial effect as compared with Ag-NPs alone.The maximum effect with a 3.2 and 5.3 fold increase was seen in Gentamicin and Streptomycin respectively providing the synergistic role of Ag-NPs. The results of antimicrobial studies indicated that the Ag-NPs are the metal of choice and can be effectively used in combination with antibiotics in order to improve their efficiency against various pathogenic microbes.

show abstract

Synergistic effects between silver nanoparticles and antibiotics and the mechanisms involved

Cited by 231 publications

References 36 publications

Combined efficacy of thymol and silver nanoparticles against Staphylococcus aureus

Combined efficacy of thymol and silver nanoparticles against Staphylococcus aureus

Silver-loaded nanotubular structures enhanced bactericidal efficiency of antibiotics with synergistic effect in vitro and in vivo

Biosynthesis of Silver Nanoparticles Using Ganoderma Lucidum and Assessment of Antioxidant and Antibacterial Activity

Contact Info

Product

Resources

About