Synergistic effect of xylano-pectinolytic enzymes produced by a bacterial isolate in bleaching of plywood industrial waste

Agrawal, Shivankar; Yadav, Ravi Dutt; Mahajan, Ritu

doi:10.1016/j.jclepro.2016.01.067

Cited by 23 publications

(10 citation statements)

References 21 publications

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“…Brightness of ultrafiltered enzymes plus chemically treated pulp was enhanced by about 2.1% in comparison with non-enzyme-treated pulp. Agrawal et al (2016) have reported nearly 1% increase in brightness in case of crude enzymes plus chemically treated pulp.…”

Section: Resultsmentioning

confidence: 98%

“…Though the treatment of plywood veneer SAQ pulp with crude xylanase–pectinase synergism resulted in 25 and 23.8% less chlorine and chlorine dioxide consumption, respectively (Agrawal et al 2016), UF enzyme-treated pulp under optimized conditions, when subjected to chemical bleaching, resulted in 30 and 28.86% reduction in consumption of chlorine and chlorine dioxide, respectively, for obtaining the same % ISO brightness as obtained by the conventional chemical bleaching (Table 1). Biobleaching of mixed hardwood kraft pulp using cellulase free xylanase produced by Bacillus subtilis ASH resulted in 28.6% reduction in chlorine consumption (Sanghi et al 2009).…”

Section: Resultsmentioning

confidence: 99%

“…1a), whereas 6.0 IU xylanase and 2.4 IU pectinase per g of pulp were found to be optimum for the bleaching of SAQ veneer pulp using crude enzymes at pH 8.5 (Agrawal et al 2016). Ultrafiltration strategy resulted in the removal of low-molecular weight impurities from the crude xylanase–pectinase preparation, which was interfering with the kinetic efficiency of enzymes.…”

Section: Resultsmentioning

confidence: 99%

“…Ultrafiltered enzyme-treated SAQ pulp bleached with 30% less chlorine resulted 11.87, 10.71, 17.42, 14.55, and 5.91% increase in pulp freeness, breaking length, burst factor, tear factor, and viscosity over control, respectively (Table 2). Agrawal et al (2016) have reported 8.5, 13.4, 10.8, and 4.2% increase in breaking length, burst factor, tear factor, and viscosity, respectively, using crude xylanase–pectinase over control. Higher gains in all physical properties were observed using UF over crude enzymes, where a gain of 6.54% in pulp freeness, 2.1% in breaking length, 3.33% in burst factor, 3.02% in tear factor, 1.54% in viscosity was observed.…”

Section: Resultsmentioning

confidence: 99%

“…Pectinase helps to degrade the pectin released into the aqueous phase of pulp (Lund et al 2004). Crude xylanase–pectinase enzymes have been successfully used in biobleaching of plywood industrial waste soda anthraquinone pulp (Agrawal et al 2016) and in biobleaching of mixed hardwood kraft pulp (Kaur et al 2010). …”

Section: Introductionmentioning

confidence: 99%

See 4 more Smart Citations

Improved efficacy of ultrafiltered xylanase–pectinase concoction in biobleaching of plywood waste soda pulp

Sharma

Agrawal

Yadav³

et al. 2017

3 Biotech

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The effect of ultrafiltered xylanase–pectinase concoction produced simultaneously by a bacterial isolate using agro-waste-based media was assessed in prebleaching of plywood waste pulp. Ultrafiltered enzymes caused 12.5% reduction in kappa number at reduced enzyme dose of xylanase–pectinase (4.0–0.8 IU) per gram of pulp under optimized conditions at pH 8.5, temperature 55 °C, and treatment period of 2 h. Using this methodology, amount of Cl2–ClO2 consumption can be reduced up to 30 and 28.86%. Significant improvement in physical and optical properties of pulp was obtained along with an additional reduction in BOD and COD values up to 18.13 and 21.66% using this novel biodelignification approach. This is the first report showing the advantages of using ultrafiltered xylanase–pectinase over crude enzymes in enhancing the bleaching capacity of pulp. This study focussing on the development of good quality paper with less pollution generating strategy will definitely prove a boon for industries.

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Section: Resultsmentioning

confidence: 98%

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Improved efficacy of ultrafiltered xylanase–pectinase concoction in biobleaching of plywood waste soda pulp

Sharma

Agrawal

Yadav³

et al. 2017

3 Biotech

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Simultaneous production of industrially important alkaline xylanase‐pectinase enzymes by a bacterium at low cost under solid‐state fermentation conditions

Kaur

Varghese

Mahajan

2019

Biotech and App Biochem

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Simultaneous production of alkaline xylanase and all seven types of pectinases by a bacterial isolate, under solid-state fermentation was checked in this study. Under optimized conditions, high concurrent production of xylanase (22,800 ± 578 IU/g substrate) and pectinase (4,832 ± 189 IU/g substrate) was achieved. The different types of pectinases produced were exo-polymethylgalacturonase (782 IU/g), endo-polymethylgalacturonase (6.42 U/g), exo-polygalacturonase (2,250 IU/g), endo-polygalacturonase (11.57 U/g), polymethylgalacturonate lyase (53.99 IU/g), polygalacturonate lyase (59.78 IU/g), and pectin esterase (5.78 IU/g). Wheat bran resulted in the highest titer of both enzymes. The maximum xylanase-pectinase yield was detected after 7 days of incubation with 2 mM MgSO 4 and 1.5 g/L K 2 HPO 4 at wheat bran to moisture ratio 1:1.5 (w/v), media to flask volume ratio 1:25, pH 7.0, temperature 37 • C, and inoculum size 15%. Xylanase was most stable at pH 8.0, retained more than 75% activity up to 24 H, whereas pectinase was most stable at pH 9.0, having full activity even after 24 H. At 45 • C, the xylanase showed 82% residual activity after 6 H of incubation. The pectinase was 97% and 61% stable up to 3 H at 50 and 55 • C, respectively. This is the first report showing the production of xylanase-pectinases by bacterium along with high titer of seven types of pectinases, suitable for industries.

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A novel and cost‐effective methodology for enhanced production of industrially valuable alkaline xylano‐pectinolytic enzymes cocktail in short solid‐state fermentation cycle

Agrawal

Varghese

Mahajan

2019

Biotechnology Progress

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The aim of this study was to enhance the production of xylano‐pectinolytic enzymes concurrently and also to reduce the fermentation period. In this study, the effect of agro‐residues extract‐based inoculum on yield and fermentation time of xylano‐pectinolytic enzymes was studied. Microbial inoculum and fermentation media were supplemented with xylan and pectin polysaccharides derived from agro‐based residues. Enzymes production parameters were optimized through two‐stage statistical design approach. Under optimized conditions (temperature 37°C, pH 7.2, K2HPO4 0.22%, MgSO4 0.1%, gram flour 5.6%, substrate: moisture ratio 1:2, inoculum size 20%, agro‐based crude xylan in production media 0.45%, and agro‐based crude xylan–pectin in inoculum 0.13%), nearly 28,255 ± 565 and 9,202 ± 193 IU of xylanase and pectinase, respectively, were obtained per gram of substrate in a time interval of 6 days only. The yield of both xylano‐pectinolytic enzymes was enhanced along with a reduction of nearly 24 h in fermentation time in comparison with control, using polysaccharides extracted from agro‐residues. The activity of different types of pectinase enzymes such as exo‐polymethylgalacturonase (exo‐PMG), endo‐PMG, exo‐polygalacturonase (exo‐PG), endo‐PG, pectin lyase, pectate lyase, and pectin esterase was obtained as 1,601, 12.13, 5637, 24.86, 118.62, 124.32, and 12.56 IU/g, respectively, and was nearly twofold higher than obtained for all seven types in control samples. This is the first report mentioning the methodology for enhanced production of xylano‐pectinolytic enzymes in short solid‐state fermentation cycle using agro‐residues extract‐based inoculum and production media.

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Synergistic effect of xylano-pectinolytic enzymes produced by a bacterial isolate in bleaching of plywood industrial waste

Cited by 23 publications

References 21 publications

Improved efficacy of ultrafiltered xylanase–pectinase concoction in biobleaching of plywood waste soda pulp

Improved efficacy of ultrafiltered xylanase–pectinase concoction in biobleaching of plywood waste soda pulp

Simultaneous production of industrially important alkaline xylanase‐pectinase enzymes by a bacterium at low cost under solid‐state fermentation conditions

A novel and cost‐effective methodology for enhanced production of industrially valuable alkaline xylano‐pectinolytic enzymes cocktail in short solid‐state fermentation cycle

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