Synergistic effect of Trichoderma and Rhizobium on Both Biocontrol of Chocolate Spot Disease and Induction of Nodulation, Physiological Activities and Productivity of Vicia faba

Saber, WesamEldin I. A.; El-Hai, K.M. Abd; Ghoneem, Khalid M.

doi:10.3923/jm.2009.286.300

Cited by 38 publications

(32 citation statements)

References 30 publications

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“…Thus, seed soaking with Fu + Tv + Rh, Rh + Tv and Tv + Fu were more effective revealing total chlorophyll with averages of 2.49, 2.22 and 2.17 mg/g fresh weight, respectively. The present findings are harmony with those obtained by Saber et al (2009) who reported that Chl. b and total Chl.…”

Section: Some Physiological Activities As Effected By Combined Bioagesupporting

confidence: 92%

POTENTIAL OF Trichoderma viride AND Rhizobium leguminosarum IN COMBINATION WITH TOPSIN M70 FUNGICIDE FOR MANAGEMENT DAMPING-OFF DISEASE OF PEA PLANTS CAUSED BY Rhizoctonia solani

El-Wahab¹,

El-Sayed²

2018

Zagazig Journal of Agricultural Research

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Efficacies of Trichoderma viride, Rhizobium leguminosarum and the fungicide Topsin M70% individually and/or their mixtures were tested in vitro and greenhouse conditions to control damping-off and root rot diseases of pea plants (Pisum sativum L., cv. Master P) caused by Rhizoctonia solani. The ability of tested Rhizobium leguminosarum and Trichodermia viride to exhibit plant growth promoting rhizobacteria (PGPR)-properties including ability to solubilize-P and production of IAA, as well as production of siderophores, hydrocyanic acid (HCN) and secretion of cell-wall degrading enzymes (chitinase and protease) were investigated. Also, under in vitro conditions the effect of Topsin M70% on growth of R. solani and T. viride, R. leguminosarum and their mixtures was determined. The fungicide effective concentration was found to range from 10 to 50 ppm for R. solani and T. viride mycelial growth being 83.30, 100, 76.08 and 100% at 40 and 50 ppm of Topsin M70%. The same trend was obtained with R. leguminosarum that showed maximum tolerance at 40 and 50 ppm of Topsin M70% with the average of 52.19 and 59.85% inhibition over control, respectively. Additionally, greenhouse conditions were conducted on sandy clay soil at Etay El Baroud Agricultural Research Station, Beheria Government, Egypt to study the singly effect of seed soaking in T. viride, R. leguminosarum or their mixtures with the soil drench fungicide in pots after sowing of pea cv. Master P in concern. Significant decrease of damping-off and root rot of pea was obtained. Numbers of survived plants, shoot length as well as, fresh and dry weight were recorded. The combined treatments R. leguminosarum + Topsin M70%, T. viride + Topsin M70% and R. leguminosarum + T. viride + Topsin M70% were the most effective ones resulting the least percentage of total dampingoff and the highest percentage of healthy plants being 96.67 and100.00%, respectively. Seed treatment with T. viride, R. leguminosarum and drenched fungicide individually or in mixture improved plant growth as indicated by the increased growth parameters and the physiological activities (phytosynthetic pigments, peroxidase and polyphenol oxidase), especially in combined treatments. Peroxidase and polyphenol oxidase activities were increased in the different treatments-even, the fungicide drench treatment. Reduction in total damping-off was positively correlated with both peroxidase (R 2 = 96.70, P ˂ 0.005) and polyphenol oxidase (R 2 = 89.60, P ˂ 0.005) activities.

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Section: Some Physiological Activities As Effected By Combined Bioagesupporting

confidence: 92%

POTENTIAL OF Trichoderma viride AND Rhizobium leguminosarum IN COMBINATION WITH TOPSIN M70 FUNGICIDE FOR MANAGEMENT DAMPING-OFF DISEASE OF PEA PLANTS CAUSED BY Rhizoctonia solani

El-Wahab¹,

El-Sayed²

2018

Zagazig Journal of Agricultural Research

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“…for plant growth and yield have been observed in greenhouse and field experiments, especially when certain isolates are used in combination (Harman et al 2004;Hoyos-Carvajal et al 2009). Reduced sheath blight and increased yields have been observed in rice (Mathivanan et al 2005) and other crops (Raj et al 2005;Saber et al 2009;Tchameni et al 2011), although the mechanisms involved mechanisms are not yet fully understood. There are many variables at play in the complex interactions between host-pathogen-antagonists under field conditions.…”

Section: Yieldmentioning

confidence: 94%

Biocontrol of sheath blight by Trichoderma asperellum in tropical lowland rice

França

Cardoso

Lustosa

et al. 2014

Agron. Sustain. Dev.

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Crop damage by rice sheath blight, Rhizoctonia solani, can decrease rice yield by up to 45 %. The classical control method of rice sheath blight in the Amazon region is the application of fungicides. Therefore, we tested here the efficiency of a biocontrol agent, Trichoderma asperellum, and fungicides. Two experiments of rice cultivation were carried out with seven treatments: four isolates of T. asperellum, a mixture of the four isolates, the fungicide pencycuron, and the control. The first experiment involved a randomized block design, and seed and foliar spray on all plots. The second experiment involved a split-plot design with foliar spray in main plots and the 1-2 foliar sprays in subplots. Results show that all treatments reduced sheath blight progression rate. In the randomized block experiment T. asperellum reduced disease severity by 19 %, increased grain weight by 34 %, and increased yield by 41 %. In the split-plot design experiment, the mixture of the four T. asperellum isolates grain reduced disease severity by 26 %, increased grain weight by 18.5 %, and increased yield by 26 %. Our results show for the first time that a mixed isolates of T. asperellum was efficient in reducing disease severity and increasing yield and grain weight.

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“…Polyphenoloxidase enzyme is involved in the oxidation of polyphenols into quinones (antimicrobial compounds) and lignification of plant cells during microbial invasion, and also may participate in the responding defense reaction and hypersensitivity by inducing plant resistance against fungi. Peroxidase is a component of an early response of plants to pathogen infection and plays a major role in the biosynthesis and polymerization of proteins and lignin or suberin cell wall constructing a physical barrier that could prevent pathogen penetration of cell walls or movement through vessels [19,20]. On the other hand, variation in photosynthetic pigments may occur by enhancing the efficacy of photosynthetic apparatus with a better potential for disease resistance and decrease in photophosphorylation rate usually occurring after infection [19].…”

Section: Acta Biologica Hungarica 66 2015mentioning

confidence: 99%

“…Peroxidase is a component of an early response of plants to pathogen infection and plays a major role in the biosynthesis and polymerization of proteins and lignin or suberin cell wall constructing a physical barrier that could prevent pathogen penetration of cell walls or movement through vessels [19,20]. On the other hand, variation in photosynthetic pigments may occur by enhancing the efficacy of photosynthetic apparatus with a better potential for disease resistance and decrease in photophosphorylation rate usually occurring after infection [19]. It could be said that the presence of B. subtilis alongside R. solani reduced its harmful impact on plants.…”

Section: Acta Biologica Hungarica 66 2015mentioning

confidence: 99%

Chitinase production byBacillus subtilisATCC 11774 and its effect on biocontrol ofRhizoctoniadiseases of potato

Saber¹,

Ghoneem²,

Al-Askar³

et al. 2015

Acta Biologica Hungarica

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Stem canker and black scurf of potato, caused by Rhizoctonia solani, can be serious diseases causing an economically significant damage. Biocontrol activity of Bacillus subtilis ATCC 11774 against the Rhizoctonia diseases of potato was investigated in this study. Chitinase enzyme was optimally produced by B. subtilis under batch fermentation conditions similar to those of the potato-growing soil. The maximum chitinase was obtained at initial pH 8 and 30 °C. In vitro, the lytic action of the B. subtilis chitinase was detected releasing 355 μg GlcNAc ml -1 from the cell wall extract of R. solani and suggesting the presence of various chitinase enzymes in the bacterial filtrate. In dual culture test, the antagonistic behavior of B. subtilis resulted in the inhibition of the radial growth of R. solani by 48.1% after 4 days. Moreover, the extracted B. subtilis chitinase reduced the growth of R. solani by 42.3% when incorporated with the PDA plates. Under greenhouse conditions, application of a bacterial suspension of B. subtilis at 10 9 cell mL -1 significantly reduced the disease incidence of stem canker and black scurf to 22.3 and 30%, respectively. In addition, it significantly improved some biochemical parameters, growth and tubers yield. Our findings indicate two points; firstly, B. subtilis possesses a good biocontrol activity against Rhizoctonia diseases of potato, secondly, the harmonization and suitability of the soil conditions to the growth and activity of B. subtilis guaranteed a high controlling capacity against the target pathogen.

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Synergistic effect of Trichoderma and Rhizobium on Both Biocontrol of Chocolate Spot Disease and Induction of Nodulation, Physiological Activities and Productivity of Vicia faba

Cited by 38 publications

References 30 publications

POTENTIAL OF Trichoderma viride AND Rhizobium leguminosarum IN COMBINATION WITH TOPSIN M70 FUNGICIDE FOR MANAGEMENT DAMPING-OFF DISEASE OF PEA PLANTS CAUSED BY Rhizoctonia solani

POTENTIAL OF Trichoderma viride AND Rhizobium leguminosarum IN COMBINATION WITH TOPSIN M70 FUNGICIDE FOR MANAGEMENT DAMPING-OFF DISEASE OF PEA PLANTS CAUSED BY Rhizoctonia solani

Biocontrol of sheath blight by Trichoderma asperellum in tropical lowland rice

Chitinase production byBacillus subtilisATCC 11774 and its effect on biocontrol ofRhizoctoniadiseases of potato

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