Synergistic effect of radachlorin mediated photodynamic therapy on propolis induced apoptosis in AMC-HN-4 cell lines via caspase dependent pathway

Ahn, Jin‐Chul; Biswas, Ratna; Chung, Phil‐Sang

doi:10.1016/j.pdpdt.2013.01.005

Cited by 13 publications

(10 citation statements)

References 24 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In our previous study, we demonstrated that DHA induces apoptosis by up-regulating the expression of Bax, down-regulating Bcl-2, Bcl-xL and procaspase-3 and increasing caspase-9 activation and induced cell cycle arrest by down-regulating cyclin E, CDK2 and CDK4 [17]. Furthermore, PDT induces apoptosis in esophageal cancer cells [5], hepatic cancer cells [26], pancreatic cancer cells [27] as well as the human head and neck cancer cells AMC-HN-4 [28]. Similarly, in the present study, we found that 5-ALA-PDT induced apoptosis in esophageal cancer cells.…”

Section: Discussionmentioning

confidence: 99%

Dihydroartemisinin Accentuates the Anti-Tumor Effects of Photodynamic Therapy via Inactivation of NF-κB in Eca109 and Ec9706 Esophageal Cancer Cells

Zhou

et al. 2014

Cell Physiol Biochem

View full text Add to dashboard Cite

Background: Photodynamic therapy (PDT) is a new treatment for esophageal cancer which has been shown to be effective in the elimination of tumor. However, PDT could induce the activation of nuclear factor-kappa B (NF-κB) in many photosensitizers based PDT, which plays a negative role in PDT. In addition, our previous results have shown that dihydroartemisinin (DHA), which was the most potent one of artemisinin derivatives, has anticancer activity in esophageal cancer cells. Methods: Cell viability was determined by MTT analysis, and apoptosis was evaluated by flow cytometry. Nuclear extract was obtained for determining NF-κB DNA-binding activity, while total protein extract obtained for downstream gene expression by western blot. Results: We demonstrated DHA enhanced PDT-induced growth inhibition and apoptosis in both human esophageal cancer cell lines Eca109 and Ec9706 in vitro. The mechanism was at least partially due to DHA deactivated PDT-induced NF-κB activation, so as to decrease tremendously the expression of its target gene Bcl-2. Conclusion: Our results demonstrate that DHA augments PDT-induced growth inhibition and apoptosis in esophageal cancer cells, and that inactivation of NF-κB activity is a potential mechanism by which DHA sensitizes esophageal cancer cells to PDT-induced growth inhibition and apoptosis.

show abstract

Section: Discussionmentioning

confidence: 99%

Dihydroartemisinin Accentuates the Anti-Tumor Effects of Photodynamic Therapy via Inactivation of NF-κB in Eca109 and Ec9706 Esophageal Cancer Cells

Zhou

et al. 2014

Cell Physiol Biochem

View full text Add to dashboard Cite

show abstract

“…The treatment mechanism involves the injection of a non-toxic photosensitizer (PS) locally, systemically or topically to a specific lesion accompanied by the absorption of visible light of a particular wavelength in the presence of oxygen from the singlet state to the triplet state as a means of generating cytotoxic reactions [18]. These reactions form reactive oxygen species (ROS) which result in tissue destruction, pathogenic microbes and cell death [19,20] (Figure 1). Photo activation may destroy cancer cells through apoptosis, necrosis or autophagy based on the organelle which the PS has accumulated [21].…”

Section: Introductionmentioning

confidence: 99%

mTOR Signaling Pathway in Cancer Targets Photodynamic Therapy In Vitro

Ayuk

Abrahamse

2019

Cells

View full text Add to dashboard Cite

The Mechanistic or Mammalian Target of Rapamycin (mTOR) is a major signaling pathway in eukaryotic cells belonging to the P13K-related kinase family of the serine/threonine protein kinase. It has been established that mTOR plays a central role in cellular processes and implicated in various cancers, diabetes, and in the aging process with very poor prognosis. Inhibition of the mTOR pathway in the cells may improve the therapeutic index in cancer treatment. Photodynamic therapy (PDT) has been established to selectively eradicate neoplasia at clearly delineated malignant lesions. This review highlights recent advances in understanding the role or regulation of mTOR in cancer therapy. It also discusses how mTOR currently contributes to cancer as well as future perspectives on targeting mTOR therapeutically in cancer in vitro.

show abstract

“…Moreover, chlorin e6 that plays a role of solubilizer of radachlorin, therefore, provides a better solubility in aqueous solution with a better self life that makes dilution of the drug as well as treatment in photodynamic therapy easier for clinicians . Therefore, this photosensitizer is studied widely and applied for tumor diagnosis as well as photodynamic therapy in recent years . Radachlorin‐mediated photodynamic therapy has been reported to have an anticancer efficacy in various malignancies like skin, lung, head and neck and thyroid cancer .…”

Section: Introductionmentioning

confidence: 99%

Chlorin e6 Derivative Radachlorin Mainly Accumulates in Mitochondria, Lysosome and Endoplasmic Reticulum and Shows High Affinity toward Tumors in Nude Mice in Photodynamic Therapy

Biswas

Moon

Ahn

2014

Photochem & Photobiology

Self Cite

View full text Add to dashboard Cite

The efficacy of photodynamic therapy (PDT) depends upon the amount of photosensitizer accumulated in the malignant tissues. Radachlorin is a popular photosensitizer used in photodynamic therapy to treat various types of cancer. In this study, we have studied the main organelles responsible for the accumulation of radachlorin in human anaplastic thyroid cancer in vitro and in vivo. The optimal time window for uptake and clearance of radachlorin also was studied. Confocal microscopic images confirmed that the radachlorin is mainly acquired by mitochondria and partially by lysosome and endoplasmic reticulum. Studies also showed that the maximum amount of radachlorin was accumulated within 3-6 h after the treatment. Radachlorin also showed a higher affinity toward malignant tumors compared to the other organs in mice xenograft model. Uptake of radachlorin reached an optimum amount within 6 h and most of the radachlorins were also cleared from the body in next 48 h. Therefore, detailed information regarding exact accumulation sites and a time window in which maximum amount of drug is accumulated and cleared were obtained by this study. Hence, not only the efficacy of the treatment can be increased but the phototoxicity after the treatment also can be controlled.

show abstract

Synergistic effect of radachlorin mediated photodynamic therapy on propolis induced apoptosis in AMC-HN-4 cell lines via caspase dependent pathway

Cited by 13 publications

References 24 publications

Dihydroartemisinin Accentuates the Anti-Tumor Effects of Photodynamic Therapy via Inactivation of NF-κB in Eca109 and Ec9706 Esophageal Cancer Cells

Dihydroartemisinin Accentuates the Anti-Tumor Effects of Photodynamic Therapy via Inactivation of NF-κB in Eca109 and Ec9706 Esophageal Cancer Cells

mTOR Signaling Pathway in Cancer Targets Photodynamic Therapy In Vitro

Chlorin e6 Derivative Radachlorin Mainly Accumulates in Mitochondria, Lysosome and Endoplasmic Reticulum and Shows High Affinity toward Tumors in Nude Mice in Photodynamic Therapy

Contact Info

Product

Resources

About

Synergistic effect of radachlorin mediated photodynamic therapy on propolis induced apoptosis in AMC-HN-4 cell lines via caspase dependent pathway

Cited by 13 publications

References 24 publications

Dihydroartemisinin Accentuates the Anti-Tumor Effects of Photodynamic Therapy via Inactivation of NF-&#954;B in Eca109 and Ec9706 Esophageal Cancer Cells

Dihydroartemisinin Accentuates the Anti-Tumor Effects of Photodynamic Therapy via Inactivation of NF-&#954;B in Eca109 and Ec9706 Esophageal Cancer Cells

mTOR Signaling Pathway in Cancer Targets Photodynamic Therapy In Vitro

Chlorin e6 Derivative Radachlorin Mainly Accumulates in Mitochondria, Lysosome and Endoplasmic Reticulum and Shows High Affinity toward Tumors in Nude Mice in Photodynamic Therapy

Contact Info

Product

Resources

About

Dihydroartemisinin Accentuates the Anti-Tumor Effects of Photodynamic Therapy via Inactivation of NF-κB in Eca109 and Ec9706 Esophageal Cancer Cells

Dihydroartemisinin Accentuates the Anti-Tumor Effects of Photodynamic Therapy via Inactivation of NF-κB in Eca109 and Ec9706 Esophageal Cancer Cells