Synergistic Effect of Photodynamic Therapy at 400 nm and Doxycycline Against
            <i>Helicobacter Pylori</i>

Baccani, Ilaria; Faraoni, Paola; Marini, Matilde; Gnerucci, Alessio; Orsini, B.; Pecile, Patrizia; Romano, G.; Fusi, Franco; Rossolini, Gian María; Antonelli, Alberto

doi:10.2217/fmb-2019-0129

Cited by 7 publications

(4 citation statements)

References 12 publications

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“…Recent PACT applications have been developed for usage under white light (Nzambe Ta keki et al, 2016;Ringot et al, 2018;Aroso et al, 2019;Khaldi et al, 2019), blue light (Buchovec et al, 2016), and even solar light (Jia et al, 2019). Currently, PACT applications are actively pursued by several research groups, with applications in dentistry as a complement of systemic antibiotic treatments (de Freitas et al, 2016;Bechara Andere et al, 2018), as a non-invasive treatment against Helicobacter pylori (Baccani et al, 2019), and even as an environment-friendly alternative for active food packaging (i.e., biodegradable coatings for strawberries disinfection), food disinfection (i.e., curcumin derivatives for lettuce and mung beans disinfection), and other agronomical applications (i.e., porphyrinic insecticides and pesticides) (Riou et al, 2014;Buchovec et al, 2016;Glueck et al, 2017;Martinez et al, 2017).…”

Section: Introductionmentioning

confidence: 99%

Porphyrin-Loaded Lignin Nanoparticles Against Bacteria: A Photodynamic Antimicrobial Chemotherapy Application

et al. 2020

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The need for alternative strategies to fight bacteria is evident from the emergence of antimicrobial resistance. To that respect, photodynamic antimicrobial chemotherapy steadily rises in bacterial eradication by using light, a photosensitizer and oxygen, which generates reactive oxygen species that may kill bacteria. Herein, we report the encapsulation of 5,10,15,20-tetrakis(4-hydroxyphenyl)-21H,23H-porphyrin into acetylated lignin water-dispersible nanoparticles ( THPP@AcLi ), with characterization of those systems by standard spectroscopic and microscopic techniques. We observed that THPP@AcLi retained porphyrin’s photophysical/photochemical properties, including singlet oxygen generation and fluorescence. Besides, the nanoparticles demonstrated enhanced stability on storage and light bleaching. THPP@AcLi were evaluated as photosensitizers against two Gram-negative bacteria, Escherichia coli and Pseudomonas aeruginosa , and against three Gram-positive bacteria, Staphylococcus aureus , Staphylococcus epidermidis , and Enterococcus faecalis . THPP@AcLi were able to diminish Gram-positive bacterial survival to 0.1% when exposed to low white LED light doses (4.16 J/cm 2 ), requiring concentrations below 5 μM. Nevertheless, the obtained nanoparticles were unable to diminish the survival of Gram-negative bacteria. Through transmission electron microscopy observations, we could demonstrate that nanoparticles did not penetrate inside the bacterial cell, exerting their destructive effect on the bacterial wall; also, a high affinity between acetylated lignin nanoparticles and bacteria was observed, leading to bacterial flocculation. Altogether, these findings allow to establish a photodynamic antimicrobial chemotherapy alternative that can be used effectively against Gram-positive topic infections using the widely available natural polymeric lignin as a drug carrier. Further research, aimed to inhibit the growth and survival of Gram-negative bacteria, is likely to enhance the wideness of acetylated lignin nanoparticle applications.

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Section: Introductionmentioning

confidence: 99%

Porphyrin-Loaded Lignin Nanoparticles Against Bacteria: A Photodynamic Antimicrobial Chemotherapy Application

et al. 2020

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“…Their safety was confirmed by on the bench and animal test results, which is normally considered as a prerequisite to further proceed with safety and efficacy studies in a clinical setting, regardless of whether efficacy studies are performed on an animal model. Therefore, these outcomes are very encouraging for the progression of the study in humans, not only for testing the safety of the capsule, but above all, for evaluating its therapeutic efficacy, alone or in possible association with antibiotic therapy [40].…”

Section: Discussionmentioning

confidence: 91%

Ingestible light source for intragastric antibacterial phototherapy: a device safety study on a minipig model

Orsini

Busechian

Faraoni

et al. 2022

Photochem Photobiol Sci

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Helicobacter pylori gastric infections are among the most diffused worldwide, suffering from a rising rate of antibiotic resistance. In this context, some of the authors have previously designed an ingestible device in the form of a luminous capsule to perform antibacterial photodynamic inactivation in the stomach. In this study, the light-emitting capsules were tested to verify the safety of use prior to perform clinical efficacy studies. First, laboratory tests measured the capsule temperature while in function and verified its chemical resistance in conditions mimicking the gastric and gut environments. Second, safety tests in a healthy minipig model were designed and completed, to verify both the capsule integrity and the absence of side effects, associated with its illumination and transit throughout the gastrointestinal tract. To this aim, a capsule administration protocol was defined considering a total of 6 animals with n = 2 treated with 8 capsules, n = 2 treated with 16 capsules and n = 2 controls with no capsule administration. Endoscopies were performed in sedated conditions before–after every capsule administration. Biopsies were taken from the corpus and antrum regions, while the gastric cavity temperature was monitored during illumination. The bench tests confirmed a very good chemical resistance and a moderate (about 3 °C) heating of the capsules. The animal trials showed no significant effects on the gastric wall tissues, both visually and histologically, accompanied with overall good animal tolerance to the treatment. The integrity of the administered capsules was verified as well. These encouraging results pose the basis for the definition of successive trials at the clinical level. Graphical abstract

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“…Baccani et al conducted a study of 400 nm PDT combined with doxycycline against H. pylori. 78 The combined therapy produced a synergistic antibacterial effect, which improved the germicidal efficacy and decreased the light availability. Notably, doxycycline, which is a member of the tetracycline group, is also a PS.…”

Section: Resultsmentioning

confidence: 99%

Research progress in photodynamic therapy for Helicobacter pylori infection

Luo,

Liu,

Zhang

et al. 2024

Helicobacter

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Helicobacter pylori (H. pylori) is a pathogenic microorganism that colonizes the human gastric mucosa and can lead to various gastric disorders, including gastritis, gastric ulcers, and gastric cancer. However, the increasing prevalence of antibiotic resistance in H. pylori has prompted the search for alternative treatment options. Photodynamic therapy has emerged as a potential alternative therapy, thus offering the advantage of avoiding some of the side effects associated with antibiotics and effectively targeting drug‐resistant strains. In the postantibiotic era, photodynamic therapy (PDT) has shown promise as a novel treatment for H. pylori infection. This review focused on elucidating the mechanism of photodynamic therapy in the treatment of H. pylori. Additionally, we present an overview of the current research on photodynamic therapy by examining both standalone photodynamic therapy and combination therapies for H. pylori infection treatment. Furthermore, the safety profile of photodynamic therapy was also evaluated. Finally, we discuss the challenges and prospects associated with this innovative technology, with an aim to provide new insights and methodologies for the treatment of H. pylori infection.

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Synergistic Effect of Photodynamic Therapy at 400 nm and Doxycycline Against Helicobacter Pylori

Cited by 7 publications

References 12 publications

Porphyrin-Loaded Lignin Nanoparticles Against Bacteria: A Photodynamic Antimicrobial Chemotherapy Application

Porphyrin-Loaded Lignin Nanoparticles Against Bacteria: A Photodynamic Antimicrobial Chemotherapy Application

Ingestible light source for intragastric antibacterial phototherapy: a device safety study on a minipig model

Research progress in photodynamic therapy for Helicobacter pylori infection

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