Synergistic effect ofPseudomonas putidaandBacillus amyloliquefaciensameliorates drought stress in chickpea (Cicer arietinumL.)

Abstract

Two plant growth promoting rhizobacteria (PGPR) Pseudomonas putida NBRIRA and Bacillus amyloliquefaciens NBRISN13 with ability to tolerate abiotic stress along with multiple PGP traits like ACC deaminase activity, minerals solubilisation, hormones production, biofilm formation, siderophore activity were evaluated for their synergistic effect to ameliorate drought stress in chickpea. Earlier we have reported both the strains individually for their PGP attributes and stress amelioration in host plants. The present study explains in detail the possibilities and benefits of utilizing these 2 PGPR in consortium for improving the chickpea growth under control and drought stressed condition. In vitro results clearly demonstrate that both the PGPR strains are compatible to each other and their synergistic growth enhances the PGP attributes. Greenhouse experiments were conducted to evaluate the effect of inoculation of both strains individually and consortia in drought tolerant and sensitive cultivars (BG362 and P1003). The growth parameters were observed significantly higher in consortium as compared to individual PGPR. Colonization of both PGPR in chickpea rhizosphere has been visualized by using gfp labeling. Apart from growth parameters, defense enzymes, soil enzymes and microbial diversity were significantly modulated in individually PGPR and in consortia inoculated plants. Negative effects of drought stress has been ameliorated and apparently seen by higher biomass and reversal of stress indicators in chickpea cultivars treated with PGPR individually or in consortia. Findings from the present study demonstrate that synergistic application has better potential to improve plant growth promotion under drought stress conditions.

Assessing the role of endophytic bacteria in the halophyte Arthrocnemum macrostachyum salt tolerance

“…Usually, the use of a consortium for plant inoculants guarantees the highest plant growth promotion with regard to single bacterium inoculation, due to the synergistic effect of the PGP properties present in the different strains (for recent examples see, Kumar et al . , ).…”

Multistrain versus single-strain plant growth promoting microbial inoculants - The compatibility issue

“…A literature survey revealed an increasing number of examples where plant inoculation with compatible strains' mixtures of P. fluorescens and plant mutualistic bacteria (Sundaramoorthy and Balabaskar, 2012;Sundaramoorthy and Balabaskar, 2013;Rathi et al, 2015;Kumar et al, 2016;Sharma et al, 2018) or benefi cial fungi including species of Trichoderma (Thilagavathi et al, 2007, Jain et al, 2012Singh et al, 2013aSingh et al, , 2013bSingh et al, , 2014Ruano-Rosa et al, 2014;Thakkar and Saraf, 2015;Chemelrotit et al, 2017;Patel et al, 2017;Yadav et al, 2017;Jambhulkar et al, 2018), Beauveria (Karthiba et al, 2010;Senthilraja et al, 2013), Pochonia (Siddiqui et al, 2003) and Clonostachys (Karlsson et al, 2015) showed better results than inoculation with individual strains or control treatment, under controlled and fi eld conditions. Furthermore, co-inoculation of specifi c Pseudomonas strains that function as mycorrhiza helper bacteria (MHB) in combination with various arbuscular mucorrhiza fungi (AMF) promoted the growth of maize plants in fi eld conditions better than single AM inoculation (Berta et al, 2014).…”

Pseudomonas putida and Bacillus amyloliquefaciens alleviates the adverse effect of pesticides and poise soil enzymes activities in chickpea (Cicer arietinum L.) rhizosphere

“…Pesticides used in this study were Carbendazim (Car), Glyphosate (Gly) and Imidacloprid (Imi). Earlier we have performed in vitro PGP traits evaluation of both the bacterium and their synergistic effect for drought amelioration in chickpea (Kumar et al 2016).…”