Synergistic Effect of Feeding <i>Aspergillus Awamori </i>and <i>Saccharomyces Cerevisiae</i> on Growth Performance in Broiler Chickens; Promotion of Protein Metabolism and Modification of Fatty acid Profile in the Muscle

Abstract

This study was conducted to examine the effects of a combined in-feed supplementation of Aspergillus awamori and Saccharomyces cerevisiae on growth and muscle protein metabolism and fatty acid profiles in broilers. Chicks (15 d old) were fed a basal diet as control, diets supplemented with 0.05% A. awamori, 0.10% S. cerevisiae, or a combination of both (7 birds/group) for 12 days. Growth of the birds was promoted by all treatments. Synergistic effects of A. awamori and S. cerevisiae were observed on body weight gain and feed conversion, breast muscle weight, and digestibility of dietary protein. Plasma 3-methylhistidine concentrations were decreased by A. awamori and S. cerevisiae, and synergistically by the combination. Gene expressions of proteolysis-related factors in muscle were reduced by all treatments. Conversely, mRNA expressions of myosin and actin were synergistically increased by the combination. Abdominal fat and plasma triglycerides were decreased by A. awamori and the combination, but not by S. cerevisiae, while muscle fat content was increased by all treatments. Interestingly, there was a decrease in saturated fatty acids and an increase in unsaturated fatty acids in muscle in all treatment groups. This change in fatty acid profile was partially related to mRNA expression of delta-6 fatty acid desaturase in the muscle. In conclusion, the combined supplementation of A. awamori and S. cerevisiae synergistically improves growth performance by promoting muscle protein metabolism. In addition, A. awamori and S. cerevisiae modify the muscle fatty acid profile.

The Visceral Organ, Gastrointestinal Tract and Blood Characteristics in Pegagan Ducks Fed Ration Fermented by Tape Yeast with Different Moisture Content

“…The mechanism associated with the cholesterol-lowering effect of yeast could be linked with an inhibitor of 3-hydroxyl-3-methylglutaryl-coenzyme reductase (Saleh et al, 2013). In line with this, Paryad and Mahmoudi (2008) reported that dietary supplementation of 1% S. cerevisiae significantly lowered plasma cholesterol and triglyceride concentrations in broiler chickens, while HDL was increased.…”

Synergistic effect of feedingAspergillus awamoriand lactic acid bacteria on performance, egg traits, egg yolk cholesterol and fatty acid profile in laying hens

“…GPX; catalase and superoxide dismutase). These results suggest that probiotics can act as an antioxidant when fed to broiler chickens (Saleh et al 2013). …”

“…The LAB used in the study contained different strains of bacteria such as Lactobacillus paracasei (KKP 824), L. rhamnosus (KKP 825) and L. rhamnosus (KKP 826) and the concentration was (6.7 Â 10 8 cfu/g) at 1:2:2 ratios from the Institute of Agricultural and Food Biotechnology, Warsaw. The inclusion levels of AA (0.05%) and LAB (0.10%) were decided according to our previous study (Saleh et al 2013) and preliminary experiments respectively.…”

“…Thus, it is probable that the increase in oleic, linoleic and alpha-linolenic acids in the egg yolk may be due to the intestinal activities of AA and LAB. This may also refer to that AA produces desaturase which may change saturated fatty acid to unsaturated fatty acids (Richard et al 2004;Saleh et al 2013).…”

Effects of dietary physical form and dietary inclusion of probiotic and enzyme on growth performance, cellular and humoral immunity, and relative weights of lymphoid organs at early period of broiler chickens fed triticale-based diets

“…Saleh et al (2012) stated that the probiotic A. awamori improved energy and protein digestibility in broilers. In another study, these authors showed that dietary supplementation with A. awamori and S. cerevisiae improved the growth performance of broilers synergistically by increasing muscle protein metabolism (Saleh et al, 2013).…”