Synergistic effect of exogeneous and endogeneous electrostimulation on osteogenic differentiation of human mesenchymal stem cells seeded on silk scaffolds

Çakmak, Anıl Sera; Çakmak, Soner; White, James D.; Raja, Waseem; Kim, Kyung-Sook; Yiğit, Sezin; Kaplan, David L.; Gümüşderelı́oğlu, Menemşe

doi:10.1002/jor.23059

Cited by 10 publications

(8 citation statements)

References 52 publications

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“…Heat shock protein 27 (hsp27) was upregulated under the exposure to electric fields as a stress response of mesenchyme cells [26,55,56]. hsp27 impeded p53-mediated accumulation of p21, the main regulator of cellular senescence [57,58,59,60], and, therefore, suppressed cellular senescence by modulating the p53 pathway [61,62,63,64]. This report corresponds to the moderate IRS of mesenchyme cells (4.94).…”

Section: Discussionmentioning

confidence: 99%

Cell Death and Induced p53 Expression in Oral Cancer, HeLa, and Bone Marrow Mesenchyme Cells under the Exposure to Noncontact Electric Fields

Mujib¹,

Alamsyah²,

Taruno³

2017

Integr Med Int

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Background: p53 acts as a transcription factor to regulate the expression of genes that modulate various cellular activities. The proliferation of cancer cells has been inhibited under the exposure to low-intensity (18 peak-to-peak voltage) and intermediate-frequency (100 KHz) electric fields generated between 2 capacitive electrodes. Therefore, the aims of this study were to observe the molecular mechanism of cell death caused by noncontact electric field exposure and to determine whether p53 protein can serve as a biomarker for this exposure or not. Methods: Oral squamous cell carcinoma, HeLa, and bone marrow mesenchyme cells were exposed to noncontact electric fields of Electro-Capacitive Cancer Therapy (ECCT) for 24 h. To observe the mechanism of cell death caused by ECCT, immunocytochemistry of p53 was performed, and the p53 expression was evaluated using immunoreactive score (IRS) calculation. Results: Electric field exposure by ECCT increased the percentage of dead cells in oral cancer cells (18.39%), HeLa cells (6.60%), and bone marrow mesenchyme cells (34.05%) with statistical significance using the independent t test compared to each control group. The IRS of p53 in oral cancer, HeLa, and bone marrow mesenchyme cultures were 10.50, 11.25, and 4.94, respectively. Conclusion: The high IRS shown in the treated oral cancer and HeLa culture cells may suggest that p53 expression in these culture cells is associated with the cell death mechanism induced by the exposure to noncontact electric fields, and the increased cell death in these culture cells may correlate with the IRS.

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Section: Discussionmentioning

confidence: 99%

Cell Death and Induced p53 Expression in Oral Cancer, HeLa, and Bone Marrow Mesenchyme Cells under the Exposure to Noncontact Electric Fields

Mujib¹,

Alamsyah²,

Taruno³

2017

Integr Med Int

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“…The use of the IteraCoil device avoided several undesired challenges and side effects such as electrochemical reactions at the point of contact of the cells/tissue with the electrode, or the necessity to create molecular scaffolds, networks, or salt bridges, or other methods of delivering electric charges or fields to the cells [Kim et al, 2009; McCullen et al, 2010; Hronik‐Tupaj et al, 2011; Creecy et al, 2013; Cakmak et al, 2016; Zhang et al, 2016].…”

Section: Resultsmentioning

confidence: 99%

“…Previous reports were greatly focused on the usage of exogenous chemicals and biological compounds to induce osteogenic differentiation of MSPCs. The role of electrical stimulation has been demonstrated in the osteogenic differentiation of MSPCs [Wan et al, 2007; Cakmak et al, 2016]. Electrical signals of different frequencies (e.g., 15 and 1 Hz) were shown to facilitate bone restoration by stimulating osteoblasts [McCullen et al, 2010; Zeighami et al, 2019].…”

Section: Resultsmentioning

confidence: 99%

Electromagnetic Fields Generated by the IteraCoil Device Differentiate Mesenchymal Stem Progenitor Cells Into the Osteogenic Lineage

Haroutunian

Tsaghikian²,

Fedorova³

et al. 2022

Bioelectromagnetics

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Rapid advances in mesenchymal stem progenitor cells (MSPCs) have rendered impetus into the area of cell therapy and regenerative medicine. The main promise of future stem cell therapies is their reliance on autologous stem cells derived from adipose tissue, which also includes treatments of bone fractures and degeneration. The effectiveness of different electric devices utilized to reprogram MSPCs toward osteogenic differentiation has provided varying degrees of effectiveness for clinical use. Adipose tissue‐derived MSPCs were flow‐cytometrically characterized and further differentiated into osteoblasts by culturing either in growth medium with pro‐osteogenic supplements or without supplements with alternating electromagnetic field (EMF) generated by IteraCoil. IteraCoil is a multi‐solenoid coil with a specific complex geometry that creates a 3D‐EMF with desired parameters without directly applying electrodes to the cells and tissues. The flow‐cytometric analysis of highly enriched (≥95%) adipose‐derived MSPCs (CD34−, CD73+, CD90+, and CD105+) was utilized for the study. Osteoblasts and chondrocyte differentiations were then assessed by specific staining and quantified using ImageJ (National Institutes of Health). The osteoblastic differentiation of MSPCs cultured in regular medium and exposed to EMF at 0.05 and 1 kHz frequencies was compared with MSPCs cultured in a pro‐osteogenic supplemented medium. In this study, we demonstrated that EMF from IteraCoil might have affected the signaling pathways that induce the osteogenic differentiation of human adipose‐derived MSPCs in the absence of exogenous osteogenic factors. Therefore, EMF‐generated osteogenic differentiation of reprogrammed adipose‐derived autologous MSPCs may treat the loss of osteoblasts and osteoporosis and open new avenues for the development of regenerative cellular therapy. © 2022 Bioelectromagnetics Society.

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“…Concentrations of soluble ions inside the variant ion pumps, transporters, and channels are the primary sources of regulation for cell activity. These various sources generally regulate membrane proteins that are sensitive to externally induced electrical stimuli, among other stimuli [149]. Certain regulatory membrane proteins as well as enzymes are considered voltage sensing proteins.…”

Section: The Biological Response To Electric Stimulationmentioning

confidence: 99%

Electroactive polymers for tissue regeneration: Developments and perspectives

Ning

Zhou

Tan

et al. 2018

Progress in Polymer Science

247

175

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Human body motion can generate a biological electric field and a current, creating a voltage gradient of -10 to -90 mV across cell membranes. In turn, this gradient triggers cells to transmit signals that alter cell proliferation and differentiation. Several cell types, counting osteoblasts, neurons and cardiomyocytes, are relatively sensitive to electrical signal stimulation. Employment of electrical signals in modulating cell proliferation and differentiation inspires us to use the electroactive polymers to achieve electrical stimulation for repairing impaired tissues. Electroactive polymers have found numerous applications in biomedicine due to their capability in effectively delivering electrical signals to the seeded cells, such as biosensing, tissue regeneration, drug delivery, and biomedical implants. Here we will summarize the electrical characteristics of electroactive polymers, which enables them to electrically influence cellular function and behavior, including conducting polymers, piezoelectric polymers, and polyelectrolyte gels. We will also discuss the biological response to these electroactive polymers under electrical stimulation. In particular, we focus this review on their applications in regenerating different tissues, including bone, nerve, heart muscle, cartilage and skin. Additionally, we discuss the challenges in tissue regeneration applications of electroactive polymers. We conclude that electroactive polymers have a great potential as regenerative biomaterials, due to their ability to stimulate desirable outcomes in various electrically responsive cells.

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Synergistic effect of exogeneous and endogeneous electrostimulation on osteogenic differentiation of human mesenchymal stem cells seeded on silk scaffolds

Cited by 10 publications

References 52 publications

Cell Death and Induced p53 Expression in Oral Cancer, HeLa, and Bone Marrow Mesenchyme Cells under the Exposure to Noncontact Electric Fields

Cell Death and Induced p53 Expression in Oral Cancer, HeLa, and Bone Marrow Mesenchyme Cells under the Exposure to Noncontact Electric Fields

Electromagnetic Fields Generated by the IteraCoil Device Differentiate Mesenchymal Stem Progenitor Cells Into the Osteogenic Lineage

Electroactive polymers for tissue regeneration: Developments and perspectives

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