Synergistic effect of docosahexaenoic acid or conjugated linoleic acid with caffeic acid on ameliorating oxidative stress of HepG2 cells

Abstract

Exploring the synergistic effect of docosahexaenoic acid (DHA) or conjugated linoleic acid (CLA) with caffeic acid (CA) on ameliorating oxidative stress, thereby introducing CA to DHA or CLA will contribute significantly to enhance the bioactivity. We observed that DHA or CLA with CA promoted the recovery of intact individual morphology and the decline of cavities inside the nucleus and apoptosis under the observation of confocal laser scanning microscopy and fluorescent inverted microscope. The activity of intracellular antioxidant enzymes catalase (CAT) and glutathione peroxidase (GSH‐Px), lactate dehydrogenase (LDH) leakage, pyruvate and malondialdehyde and reactive oxygen species (ROS), cellular morphology, and cell cycle were analyzed. Our results showed that DHA or CLA with CA enhanced the activity of CAT and GSH‐Px, decreased LDH leakage and the number of apoptotic, significantly inhibited (ROS‐induced cellular injury. Cell arrest in G1 and G2 phase during cell mitosis was reduced by the measurement of flow cytometry. DHA or CLA combined with CA could markedly strengthen the free radical scavenging and endogenous antioxidant defense capacity on HepG2 cells. This study provides a new direction in the application of synergies to antioxidant compounds. Practical Application Caffeic acid (CA) can synergize with docosahexaenoic acid (DHA) or conjugated linoleic acid (CLA) to enhance antioxidant capacity. This study highlighted an effect of ameliorating oxidative stress injury DHA or CLA with CA on HepG2 cells. The data indicated that DHA or CLA with CA might be used to relieve oxidative stress damage.

Effects of in ovo feeding of t10,c12-conjugated linoleic acid on hepatic lipid metabolism and subcutaneous adipose tissue deposition in newly hatched broiler chicks

“…Therefore, we determined the hepatic antioxidative ability and found that 1.5 to 4.5 mg t10,c12-CLA treatment yielded higher GSH-Px and T-AOC activities and decreased the level of MDA in the liver of chicks. These findings are consistent with previous findings ( Qi et al., 2018 ; Liu et al., 2021 ). MDA is a major oxidation product of peroxidized PUFAs and an important indicator of lipid peroxidation.…”

Human Recombinant DNase I (Pulmozyme®) Inhibits Lung Metastases in Murine Metastatic B16 Melanoma Model That Correlates with Restoration of the DNase Activity and the Decrease SINE/LINE and c-Myc Fragments in Blood Cell-Free DNA

“…An interesting observation was made that Pulmozyme ® and DNase I treatment led to the violation of nuclear morphology and disruption of the structural organization of the B16 cells’ chromatin: the nuclei with internal voids were disintegrated and wrinkled and intercellular spaces were expanded ( Figure 4 C). Moreover, nuclear chromatin condensation and fragmentation with the formation of nuclear vacuoles and a large number of polymorphic granules and parietal localization of chromatin close to the nuclear membrane were observed, which characterizes the degradation of nucleosomal DNA, and DNA packing in microparticles, indicating cell death via necrosis or late-stage apoptosis, which is consistent with data in the literature [ 34 , 35 , 36 ]. The detected changes were more pronounced with the i.n.…”

“…The administration of both Pulmozyme ® and DNase I led to the activation of apoptosis in metastatic foci with an increase in cfDNA concentration. Apoptosis was confirmed by morphological changes in metastases in lungs, where DNase I and especially Pulmozyme ® caused the condensation of chromatin in the form of clumps in the nuclei, and these degenerative changes indicate cell death via necrosis or late-stage apoptosis [ 34 , 35 , 36 , 52 ].…”

Caffeic Acid, an Active Ingredient in Coffee, Combines with DOX for Multitarget Combination Therapy of Lung Cancer

“…17 Studies show that caffeic acid reduces noise-induced oxidative damage in the cochlea, 18 treats atopic dermatitis, 19 and inhibits esophageal 20 and liver cancer. 21 However, most studies focused on the signaling transduction mechanism and did not identify its target at the cell membrane.…”

“…Caffeic acid is one of the main active ingredients of coffee; in addition, the content of it in propolis, perilla, hawthorn, lemon, and other common foods is also high . Studies show that caffeic acid reduces noise-induced oxidative damage in the cochlea, treats atopic dermatitis, and inhibits esophageal and liver cancer . However, most studies focused on the signaling transduction mechanism and did not identify its target at the cell membrane.…”