Synergistic effect of co-culture rhizosphere Streptomyces: A promising strategy to enhance antimicrobial activity and plant growth-promoting function

Abstract

Rhizosphere Streptomyces is one of the important types of rhizosphere microorganisms that plays an important role in promoting plant growth and controlling plant diseases to maintain agricultural ecosystem balance and green ecological agriculture development as beneficial bacteria. Microbial co-culture simulates the complex biocommunity in nature, which has more advantages than the monoculture with a synergistic effect. As the key signal mediums of microorganisms, plants, and their interactions, microbial metabolites are of great significance in revealing their functional mechanism. In this study, two potential plant growth-promoting rhizobacteria, Streptomyces albireticuli MDJK11, and Streptomyces alboflavus MDJK44, were selected to explore the effects of co-culture and monoculture on plant growth promotion and disease prevention, and the metabolic material basis was analyzed by metabonomics. Results showed that Streptomyces MDJK11, MDJK44 monoculture, and co-culture condition all showed good growth promoting and antimicrobial effects. Moreover, as compared to the monoculture, the co-culture showed the advantage of a synergistic enhancement effect. LC-MS-based metabonomics analysis showed the metabolic material bases of Streptomyces for plant growth promotion and disease prevention were mainly plant hormone and antibiotics and the co-culture condition could significantly stimulate the production of plant hormone promoters and macrolide, cyclic peptide, and aminoglycoside antibiotics. The study proved that the co-cultures of S. albireticuli MDJK11 and S. alboflavus MDJK44 have great potential in crop growth promotion and disease prevention.

Holistic Approaches to Plant Stress Alleviation: A Comprehensive Review of the Role of Organic Compounds and Beneficial Bacteria in Promoting Growth and Health

“…In a study, two potentially beneficial bacteria, Streptomyces albireticuli MDJK11 and Streptomyces alboflavus MDJK44, were selected to explore the effects of co-culture and monoculture on wheat plant growth promotion and disease prevention, and as compared to the monoculture, the co-culture showed the advantage of a synergistic enhancement effect by enhancing the function of dissolving phosphorus and nitrogen and promoting the growth of wheat roots and seedlings. It also showcases a better control ability over wheat, corn, and tobacco diseases infected by pathogenic fungi [ 65 ].…”

Streptomyces at the Heart of Several Sectors to Support Practical and Sustainable Applications: A Review

“…Overall, results indicate that Streptomyces can be used as a potential bio-fertilizer that may replace conventional fertilizer in various situations [21] . Further research is required to develop a formulation that contains these viable strains in combination with other beneficial microorganisms (co-culture) [300] for commercial application.…”

Whole genome analysis of Streptomyces sp. RerS4, a Rehmannia glutinosa rhizosphere microbe producing a new lipopeptide

“…Rhizosphere Streptomyces , one of the most important genera of actinomycetes, are mostly beneficial microorganisms abundant in plant rhizosphere soil. In recent years, rhizosphere Streptomyces species have been considered abundant resources of biofungicides or biofertilizers for agricultural usage due to (1) their production of various active compounds with agricultural applications, (2) their multiple strategies to suppress fungal pathogens, such as nutrient competition, cell wall degradation, virulence factor degradation and plant immunity induction, and (3) their nutrient absorption, all of which in turn boost plant development by producing auxins, solubilizing inorganic phosphate, fixing nitrogen and other methods [ [10] , [11] , [12] , [13] ]. Among them, the method of releasing antibiotics and antimicrobial peptides by Streptomyces has attracted much attention.…”