Synergistic effect of a drug loaded electrospun patch and systemic chemotherapy in pancreatic cancer xenograft

Abstract

Pancreatic cancer has a high rate of local recurrence and poor prognosis even with adjuvant chemotherapy after curative resection. The aim of this study was to investigate if local drug delivery combined with low dose systemic chemotherapy can increase the therapeutic effect of chemotherapy while reducing systemic toxicities. Poly-L-lactic acid-based 5-FU releasing patch was fabricated by electrospinning, and its tumour killing effects were first confirmed in vitro. The 5-FU patch directly adhered to the tumour in subcutaneous and orthotopic murine models, and induced a significant decrease in tumour size. Systemic gemcitabine treatment group, 5-FU drug releasing patch group, and systemic gemcitabine plus 5-FU patch group were compared by tumour size measurement, non-invasive bio-imaging, and histology in subcutaneous models. Combination of local drug patch and systemic chemotherapy led to increased tumour suppression effects that lasted longer, as well as increased survival rate. Histology revealed higher degree of apoptosis in the combined group. Systemic toxicity was recovered within 7 days after the treatment in all mice. Conclusively, local drug delivery using biocompatible polymer patch significantly inhibited tumour growth, and combination with systemic chemotherapy was more effective than single systemic chemotherapy.

Biocompatibility of electrospun cell culture scaffolds made from balangu seed mucilage/PVA composites

“…Similar notions have been obtained in a recent study by tailoring the polymeric scaffold geometry in 3D grid PCL architectures where the significantly improved neurite outgrowth of the PC12 cells was successfully directed the long arm direction of the designed fibrous grid [60]. Typically, in a local drug delivery study using a biocompatible poly-L-lactic acid-based 5-FU release patch, the electrospun system has yielded a significant tumour suppression effect in murine models [61]. Apart from tuning of composition and biocompatibility of the nanofibrous cell culture scaffolds, there is accumulating evidence for the importance of electrospun fibers in other aspects of nanotechnology, such drug delivery applications, which could be suggested as a prospective application for BSM/PVA nanofibers.…”

Anticancer effect of locally applicable aptamer‐conjugated gemcitabine‐loaded atelocollagen patch in pancreatic cancer patient–derived xenograft models

“…They found that loaded gemcitabine was rapidly released within the first 10 hours followed by a sustained release over the next 134 hours, and time‐dependent inhibition of tumor spheroid growth and cell viability. Jun et al 26 , 27 fabricated a poly‐L‐lactic acid–based 5‐FU–releasing patch by electrospinning and tested the oncologic effect of the combination of a local drug patch and systemic chemotherapy. This combination treatment resulted in increased tumor suppression effects that lasted longer, as well as increased survival rate, showing potential benchmarking for future clinical practice.…”

A Multilayer Nanofibrous Mat for the Topical Chemotherapy of the Positive Margin in Bladder Cancer

“… 19 The application of medicinal mats for antitumors mainly remains in laboratory. 20–22 Localized medicinal patches may be a practical choice for BC with frequent local recurrence. Herein, we utilized sequential electrospinning to fabricate a multilayer mat, in which GEM and CDDP were electrospun individually in distinct layers.…”