Synergistic Effect Induced by Gold Nanoparticles with Polyphenols Shell during Thermal Therapy: Macrophage Inflammatory Response and Cancer Cell Death Assessment

Matteis, Valeria De; Cascione, Mariafrancesca; Rizzello, Loris; Manno, D.; Guglielmo, Claudia Di; Rinaldi, R.

doi:10.3390/cancers13143610

Cited by 15 publications

(10 citation statements)

References 98 publications

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“…In addition, our results showed that the toxic behavior was evident at low doses of conventional Ag NPs. This evidence was demonstrated in our previous work in which the same plant extract was used to achieve Au NPs tested on THP-1 cells [80]. In addition, a lot of studies reported the employment of similar low doses of Ag NPs in vitro [81] and in vivo [82][83][84].…”

Section: 𝑤 =supporting

confidence: 58%

“…Surprisingly, the Ag NPs obtained by green Therefore, the data showed that plant extracts not only were responsible for the reduction and stabilization of cells but also acting as therapeutic agents [92]. The combined action between the green compounds (polyphenols, proteins, and others) and Ag NPs showed a synergistic effect against cancer cell lines [80]. On the other hand, the biomolecules protected the healthy and immune cells from the toxicity and inflammatory properties correlated to the Ag NPs [93].…”

Section: Discussionmentioning

confidence: 99%

“…showed a synergistic effect against cancer cell lines [80]. On the other hand, the biomolecules protected the healthy and immune cells from the toxicity and inflammatory properties correlated to the Ag NPs [93].…”

Section: 𝑤 =mentioning

confidence: 98%

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Green Silver Nanoparticles Promote Inflammation Shutdown in Human Leukemic Monocytes

et al. 2022

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The use of silver nanoparticles (Ag NPs) in the biomedical field deserves a mindful analysis of the possible inflammatory response which could limit their use in the clinic. Despite the anti-cancer properties of Ag NPs having been widely demonstrated, there are still few studies concerning their involvement in the activation of specific inflammatory pathways. The inflammatory outcome depends on the synthetic route used in the NPs production, in which toxic reagents are employed. In this work, we compared two types of Ag NPs, obtained by two different chemical routes: conventional synthesis using sodium citrate and a green protocol based on leaf extracts as a source of reduction and capping agents. A careful physicochemical characterization was carried out showing spherical and stable Ag NPs with an average size between 20 nm and 35 nm for conventional and green Ag NPs respectively. Then, we evaluated their ability to induce the activation of inflammation in Human Leukemic Monocytes (THP-1) differentiated into M0 macrophages using 1 µM and 2 µM NPs concentrations (corresponded to 0.1 µg/mL and 0.2 µg/mL respectively) and two-time points (24 h and 48 h). Our results showed a clear difference in Nuclear Factor κB (NF-κb) activation, Interleukins 6–8 (IL-6, IL-8) secretion, Tumor Necrosis Factor-α (TNF-α) and Cyclooxygenase-2 (COX-2) expression exerted by the two kinds of Ag NPs. Green Ag NPs were definitely tolerated by macrophages compared to conventional Ag NPs which induced the activation of all the factors mentioned above. Subsequently, the exposure of breast cancer cell line (MCF-7) to the green Ag NPs showed that they exhibited antitumor activity like the conventional ones, but surprisingly, using the MCF-10A line (not tumoral breast cells) the green Ag NPs did not cause a significant decrease in cell viability.

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Section: 𝑤 =supporting

confidence: 58%

Section: Discussionmentioning

confidence: 99%

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Green Silver Nanoparticles Promote Inflammation Shutdown in Human Leukemic Monocytes

et al. 2022

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“…We used Laurus nobilis , a native plant from the Mediterranean area, containing several clusters of phytochemicals [ 50 ]. These molecules are directly involved as reducing agents for the eco-friendly synthesis of SiO 2 NPs [ 51 ], making the process cheap, time-saving, and sustainable [ 52 ].…”

Section: Resultsmentioning

confidence: 99%

High Doses of Silica Nanoparticles Obtained by Microemulsion and Green Routes Compromise Human Alveolar Cells Morphology and Stiffness Differently

Matteis

Cascione

Luca

et al. 2022

Bioinorganic Chemistry and Applications

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Among all the inorganic nanomaterials used in commercial products, industry, and medicine, the amorphous silica nanoparticles (SiO2 NPs) appeared to be often tolerated in living organisms. However, despite several toxicity studies, some concerns about the exposure to high doses of SiO2 NPs with different sizes were raised. Then, we used the microemulsion method to obtain stable SiO2 NPs having different sizes (110 nm, 50 nm, and 25 nm). In addition, a new one-pot green synthetic route using leaves extract of Laurus nobilis was performed, obtaining monodispersed ultrasmall SiO2 NPs without the use of dangerous chemicals. The NPs achieved by microemulsion were further functionalized with amino groups making the NPs surface positively charged. Then, high doses of SiO2 NPs (1 mg/mL and 3 mg/mL) achieved from the two routes, having different sizes and surface charges, were used to assess their impact on human alveolar cells (A549), being the best cell model mimicking the inhalation route. Cell viability and caspase-3 induction were analyzed as well as the cellular uptake, obtaining that the smallest (25 nm) and positive-charged NPs were more able to induce cytotoxicity, reaching values of about 60% of cell death. Surprisingly, cells incubated with green SiO2 NPs did not show strong toxicity, and 70% of them remained vital. This result was unusual for ultrasmall nanoobjects, generally highly toxic. The actin reorganization, nuclear morphology alteration, and cell membrane elasticity analyses confirmed the trend achieved from the biological assays. The obtained data demonstrate that the increase in cellular softness, i.e., the decrease in Young’s modulus, could be associated with the smaller and positive NPs, recording values of about 3 kPa. On the contrary, green NPs triggered a slight decrease of stiffness values (c.a. 6 kPa) compared to the untreated cells (c.a. 8 kPa). As the softer cells were implicated in cancer progression and metastasization, this evidence strongly supported the idea of a link between the cell elasticity and physicochemical properties of NPs that, in turn, influenced the interaction with the cell membrane. Thus, the green SiO2 NPs compromised cells to a lesser extent than the other SiO2 NPs types. In this scenario, the elasticity evaluation could be an interesting tool to understand the toxicity of NPs with the aim of predicting some pathological phenomena associated with their exposure.

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“…For this purpose, the improved techniques of electroporation, antibody-based vectors and also HSP70 inhibitor-loaded microcarriers on the basis of liposomes, nanoparticles, dendrimers and others may be applied. The results obtained on breast cancer-related models with certain kinds of formulation including nanoparticles [ 172 , 173 , 176 , 188 ] seem hopeful, so further research work in those directions may yield a success. Moreover, the approach with targeted delivery is suitable for not only the small molecule inhibitors but also specific gene vectors constructed for the HSP70 downregulation in breast tumors.…”

Section: Approaches To Targeting Hsp70s To Fight Breast Cancermentioning

confidence: 99%

HSP70s in Breast Cancer: Promoters of Tumorigenesis and Potential Targets/Tools for Therapy

Kabakov

Gabai²

2021

Cells

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The high frequency of breast cancer worldwide and the high mortality among women with this malignancy are a serious challenge for modern medicine. A deeper understanding of the mechanisms of carcinogenesis and emergence of metastatic, therapy-resistant breast cancers would help development of novel approaches to better treatment of this disease. The review is dedicated to the role of members of the heat shock protein 70 subfamily (HSP70s or HSPA), mainly inducible HSP70, glucose-regulated protein 78 (GRP78 or HSPA5) and GRP75 (HSPA9 or mortalin), in the development and pathogenesis of breast cancer. Various HSP70-mediated cellular mechanisms and pathways which contribute to the oncogenic transformation of mammary gland epithelium are reviewed, as well as their role in the development of human breast carcinomas with invasive, metastatic traits along with the resistance to host immunity and conventional therapeutics. Additionally, intracellular and cell surface HSP70s are considered as potential targets for therapy or sensitization of breast cancer. We also discuss a clinical implication of Hsp70s and approaches to targeting breast cancer with gene vectors or nanoparticles downregulating HSP70s, natural or synthetic (small molecule) inhibitors of HSP70s, HSP70-binding antibodies, HSP70-derived peptides, and HSP70-based vaccines.

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Synergistic Effect Induced by Gold Nanoparticles with Polyphenols Shell during Thermal Therapy: Macrophage Inflammatory Response and Cancer Cell Death Assessment

Cited by 15 publications

References 98 publications

Green Silver Nanoparticles Promote Inflammation Shutdown in Human Leukemic Monocytes

Green Silver Nanoparticles Promote Inflammation Shutdown in Human Leukemic Monocytes

High Doses of Silica Nanoparticles Obtained by Microemulsion and Green Routes Compromise Human Alveolar Cells Morphology and Stiffness Differently

HSP70s in Breast Cancer: Promoters of Tumorigenesis and Potential Targets/Tools for Therapy

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