Synergistic effect between catechin and ciprofloxacin on chronic bacterial prostatitis rat model

Abstract

Background : Chronic bacterial prostatitis (CBP) is the most common urological disease in adult men. Antibiotic therapy is the gold standard of treatment. However, long-term therapy resulted in many side-effects and bacterial resistance. Because of these reasons, we need new treatment modality that could replace traditional antibiotic therapy. Catechin, an extract of green tea, has antimicrobial effect against various bacteria and synergy effect to antibiotics. We evaluate the synergistic effects of catechin on the treatment of CBP in an animal model. Methods : An experimental CBP model was induced in 70 male Wistar rats by instillation of 0.2 mL bacterial suspension (E-coli Z17, O2:K1:H-) containing 1 ¥ 108 CFU/mL into the prostatic urethra. Microbiologically and histologically proven CBP model was demonstrated in 58.6% (41 of 70) of the rats after 4 weeks of bacterial instillation. The 41 rats demonstrating CBP were randomly divided into four groups; the control, catechin, ciprofloxacin and catechin with ciprofloxacin groups. All drug treatments were conducted over a period of 2 weeks. After treatment, the results were analyzed with microbiological cultures and histological findings of the prostate and urine samples to compare each group. Results : Microbiological cultures and histological findings of the prostate and urine samples demonstrated reduced bacterial growth and improved inflammatory responses in all three experimental groups compared with the control group. The catechin group showed coherent trends of decrease in bacterial growth and improvements in prostatic inflammation compared with the control group, but not to a statistically significant degree ( P > 0.05). However, the ciprofloxacin and catechin with ciprofloxacin groups showed statistically significant decreases in bacterial growth and improvements in prostatic inflammation compared with the control group ( P < 0.05). The catechin with ciprofloxacin group showed a statistically significant decrease in bacterial growth and improvements in prostatic inflammation compared with the ciprofloxacin group ( P < 0.05).Conclusions : These results suggest that catechin may be an effective material in CBP treatment. Particularly, combination treatment of catechin and ciprofloxacin has synergistic effect. Therefore, we suggest that the combination of catechin and ciprofloxacin may be effective in treating CBP with a higher success rate.

Epidemiological Investigation on Duck Salmonellosis in Some Selected Areas of Bangladesh

“…The highest resistance was found with Oxacillin at all epidemiological criteria. This finding satisfy the result of Chugh and Suheir (1983), Banani et al (2003), Lee et al (2005), Kobayashi et al (2007) and Haque (2011). Among 5 antibiotics the selected isolates showed 100% sensitive to Ciprofloxacin and Azithromycin.…”

Experimental rodent models of prostatitis: limitations and potential

“…77 More recent studies have evaluated the antibacterial effects of intraprostatic zinc injection 78 and synergistic effects of catechin treatment (extract of green tea) combined with the antibiotic ciprofloxacin in the experimental rat model of chronic bacterial prostatitis. 79 Accumulated evidence suggests that rodent models of experimentally induced prostate inflammation (categories I and II) share many striking similarities to the development and persistence of human chronic bacterial prostatitis, possible comparable to the natural history of the disease. 73 Furthermore, retrograde intraurethral introduction of infectious agent appears to be the most efficient means of inducing bacterial prostatitis in rodents.…”

In vitro antibacterial and synergistic effect of phlorotannins isolated from edible brown seaweed Eisenia bicyclis against acne-related bacteria

“…Marine-derived polyphenols (phlorotannins) are the predominant EtOAc-soluble compound in brown algae (Choi et al 2010). Among EtOAc-soluble compound, the polyphenol polymers (eckol, PFF, dieckol, bacterial prostatitis model in the rat (Lee et al 2005). Based on these reports, the synergistic effect of marinederived polyphenol on P. acnes was assessed in combination with commercial antibiotics to treat acne.…”