Synergistic dual-mechanism fluorescence quenching immunochromatographic assay based on Fe-polydopamine submicrobeads for sensitive detection of enrofloxacin

High-Performance Fluorescent Microspheres Based on Fluorescence Resonance Energy Transfer Mode for Lateral Flow Immunoassays

“…At an excitation of 365 nm, TCBPEME could absorb photons and transform from the ground state S 0 to the excited state S 1 . Meanwhile, the energy was transferred from the excited state of TCBPEME to that of BAPF by the electric− dipole interaction among molecules, 29 resulting in a robust FRET phenomenon. The acceptor could be excited with a UV light source via energy conversion of the donor, considerably broadening the Stokes shift.…”

Fortified Dual-Spectral Overlap with Enhanced Colorimetric/Fluorescence Dual-Response Immunochromatography for On-Site Bimodal-Type Gentamicin Monitoring

“…15−17 Among them, colorimetric/fluorescence bimodal-type ICA with a target-dependent dual-response variation and built-in self-calibration function to avoid/reduce target-independent interferences allows easy satisfaction of the requirements of sensitive and accurate on-site monitoring. 18 Of particular note is that compared with traditional competitive-type ICA with a colorimetric "turn off" pattern, bimodal-type ICA with the fluorescence quenching ("turn on" pattern) significantly enhances the response ability to trace targets. 20 For the typical quenching mechanism in the ICA system, the prerequisite is that the signal tracer possesses favorable spectral overlap with the excitation and emission spectra of the fluorescence donor.…”

“…Growing evidence has indicated that immunochromatography (ICA) as a hand-held membrane-based detection platform has shown tremendous potential for on-site monitoring, which resulted from the advantages of readability that results in evaluation provided by a visual color change and convenience of the inspection procedure without large-scale instruments. − Among them, colorimetric/fluorescence bimodal-type ICA with a target-dependent dual-response variation and built-in self-calibration function to avoid/reduce target-independent interferences allows easy satisfaction of the requirements of sensitive and accurate on-site monitoring. , Of particular note is that compared with traditional competitive-type ICA with a colorimetric “turn off” pattern, bimodal-type ICA with the fluorescence quenching (“turn on” pattern) significantly enhances the response ability to trace targets . For the typical quenching mechanism in the ICA system, the prerequisite is that the signal tracer possesses favorable spectral overlap with the excitation and emission spectra of the fluorescence donor.…”

Dual‐Track Multifunctional Bimetallic Metal‐Organic Frameworks for Antibiotic Enrichment and Detection

“…While allowing for the rapid detection of antibiotics, methods such as colloidal gold immunochromatographic assays, [ 5 ] enzyme‐linked immunosorbent assays (ELISA), [ 6 ] and biosensing [ 7 ] present some drawbacks. For instance, biosensors, particularly colorimetric ones, often lack the necessary sensitivity for detecting trace targets and, thus, frequently rely on complex sample pretreatment procedures or signal amplification strategies to achieve high‐sensitivity detection.…”