Synergistic delivery of bFGF and BMP-2 from poly(<scp>l</scp>-lactic-<i>co</i>-glycolic acid)/graphene oxide/hydroxyapatite nanofibre scaffolds for bone tissue engineering applications

Ren, Xiaoyan; Liu, Qinyi; Zheng, Shuang; Zhu, Jiaqi; Qi, Zhiping; Fu, Chen; Yang, Xiaoyu; Zhao, Yan

doi:10.1039/c8ra05250f

Cited by 19 publications

(28 citation statements)

References 45 publications

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“…Bhahat et al applied solution blending to introduce GO and HAP into collagen and fabricated Col/GO/HAP nanocomposite scaffold via freeze drying, and found that the compressive stress and biocompatibility were improved [15] . Renet al implemented solution blending to introduce GO and HAP into poly (L-lactic-co-glycolic acid) (PLGA) and fabricated PLGA/GO/HAP nanofibre scaffold via electrospinning, and discovered that the tensile strength and cytocompatibility were improved [16] . Xiong et al treated HAP with glucosamine and then blended it with GO and sodium alginate (SA) to fabricate GO/HAP/SA nanocomposite scaffold through freeze drying, and found that the compressive properties and bioactivity of the scaffold were better than those of neat SA scaffold [17] .…”

Section: Introductionmentioning

confidence: 99%

In situ synthesis of hydroxyapatite nanorods on graphene oxide nanosheets and their reinforcement in biopolymer scaffold

Shuai

Peng

Feng

et al. 2022

Journal of Advanced Research

149

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Section: Introductionmentioning

confidence: 99%

In situ synthesis of hydroxyapatite nanorods on graphene oxide nanosheets and their reinforcement in biopolymer scaffold

Shuai

Peng

Feng

et al. 2022

Journal of Advanced Research

149

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“…In recent years, bone tissue engineering shows great potential in developing effective alternative as bone substitutes and grafts for bone defect repair. Various synthetic and natural materials, including polycaprolactone, PLGA, bioactive glasses, and chitosan, have been fabricated potential bone scaffolds that are utilized as grafts for bone defect repair [4][5][6][7]. For PLGA materials in particular, many researchers have fabricated PLGA scaffolds for bone defect repair; they show good biocompatibility, low immunogenicity, and toxicity and are widely used in pharmaceutical and tissue engineering as an FDA certificated medical materials.…”

Section: Introductionmentioning

confidence: 99%

“…Compare with graphene and rGO, GO has better dispersion within the polymer. Previous studies reported that the mechanical properties of polymer materials were improved by the addition of GO due to the interfacial interaction between the oxygen containing functional moieties of GO and the hydroxyl or amine groups of the polymer materials [5,15,34]. After PLL surface modification, it could be seen that there was no statistically significant difference in the tensile strengths of all hybrid fiber matrices before and after PLL surface modification, which indicated that the mechanical properties of hybrid fiber matrices appeared not to have been affected by the PLL surface modification.…”

mentioning

confidence: 99%

“…At the same time, in order to further improve the biological properties of hybrid fiber matrices, the GO and PLL surface modification was utilized to improve the biocompatibility of the PLGA hybrid fiber matrices. Previous studies have demonstrated that GO can effectively improve the mechanical properties, biocompatibility, and osteoinductive ability of the PLGA materials [5,15]. Furthermore, because of the positive interaction between the positive charge on the PLL surface and the negative charge on the cell membrane surface, PLL surface modification can improve the affinity between materials and cells.…”

mentioning

confidence: 99%

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Enhanced Cell Proliferation and Osteogenesis Differentiation through a Combined Treatment of Poly-L-Lysine-Coated PLGA/Graphene Oxide Hybrid Fiber Matrices and Electrical Stimulation

Zhu

Zheng

et al. 2020

Journal of Nanomaterials

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Bone tissue engineering scaffold provides an effective treatment for bone defect repair. Biodegradable bone scaffold made of various synthetic and natural materials can be used as bone substitutes and grafts for defect site, which has great potential to support bone regeneration. Regulation of cell-scaffold material interactions is an important factor for modulating the cellular activity in bone tissue engineering scaffold applications. Thus, the hydrophilic, mechanical, and chemical properties of scaffold materials directly affect the results of bone regeneration and functional recovery. In this study, a poly-L-lysine (PLL) surface-modified poly(lactic-co-glycolic acid) (PLGA)/graphene oxide (GO) (PLL-PLGA/GO) hybrid fiber matrix was fabricated for bone tissue regeneration. Characterization of the resultant hybrid fiber matrices was done using scanning electron microscopy (SEM), contact angle, and a material testing machine. According to the results obtained from the test above, the PLL-PLGA/GO hybrid fiber matrices exhibited high wettability and mechanical strength. The special surface characteristics of PLL-PLGA/GO hybrid fiber matrices were more beneficial for protein adsorption and inhibit the proliferation of pathogens. Moreover, the enhanced regulation of MC3T3-E1 cell proliferation and differentiation was observed, when the resultant hybrid fiber matrices were combined with electrical stimulation (ES). The cellular response of MC3T3-E1 cells including cell adhesion, proliferation, alkaline phosphatase (ALP) activity, calcium deposition, and osteogenesis-related gene expression was significantly enhanced with the synergistic effect of resultant hybrid fiber matrices and ES. These data indicate that the PLL-PLGA/GO hybrid fiber matrices supported the cellular response in terms of cell proliferation and osteogenesis differentiation in the presence of electrical stimulation, which could be a potential treatment for bone defect.

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“…Zhou et al [44] investigated the potential of poly(D,L-lactic acid)/collagen/ HAp composite nanofibers for bone regeneration. Ren et al [45] fabricated the electrospun poly(L-lactic-coglycolic acid)/HAp/graphene oxide for bone tissue engineering. However, there was no report about incorporation of DOX-HAp into the PVA-chitosan/PU core-shell nanofibers for bone cancer treatment in vitro.…”

Section: Introductionmentioning

confidence: 99%

Incorporation of Hydroxyapatite/Doxorubicin into the Chitosan/Polyvinyl Alcohol/Polyurethane Nanofibers for Controlled Release of Doxurubicin and Its Anticancer Property

2020

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In the present study, the doxorubicin-hydroxyapatite (DOX-HAp) has been loaded into the chitosan/PVA/PU single layer and core-shell nanofibers. The potential of synthesized nanofibers was evaluated for controlled release of DOX and bone cancer treatment in vitro. The synthesized DOX-HAp nanohybrid was characterized using XRD, SEM, and UV-Vis analysis. The morphology of synthesized nanofibers was examined by SEM and TEM analysis. For single layer nanofibers, some DOX-HAp nanohybrids were observed on the nanofibers surface. The fiber diameter was increased by increasing shell flow rate and no DOX-HAp nanohybrids were detected on the core-shell fibers surface. The DOX encapsulation efficiency of single layer and core-shell layer fibers was higher than 90 %. The initial burst release from single layer fiber at initial hours and the continuous release of DOX was observed from single layer nanofibers during 7 and 10 days under acidic and physiological pH. The sustained release of DOX was obtained within 10 and 14 days, 15 and 18 days, 21 and 25 days from core-shell fibers with flow rates of 0.3, 0.5 and 0.8 ml/h under acidic and physiological pH. The non-Fickian diffusion and Fickian diffusion were achieved from single layer and core-shell nanofibers. The cell attachment and cell death results indicated the high potential of DOX loaded-core-shell fibers for bone cancer treatment.

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Synergistic delivery of bFGF and BMP-2 from poly(l-lactic-co-glycolic acid)/graphene oxide/hydroxyapatite nanofibre scaffolds for bone tissue engineering applications

Abstract: One of the goals of bone tissue engineering is to create scaffolds with excellent biocompatibility, osteoinductive ability and mechanical properties.

Cited by 19 publications

References 45 publications

In situ synthesis of hydroxyapatite nanorods on graphene oxide nanosheets and their reinforcement in biopolymer scaffold

In situ synthesis of hydroxyapatite nanorods on graphene oxide nanosheets and their reinforcement in biopolymer scaffold

Enhanced Cell Proliferation and Osteogenesis Differentiation through a Combined Treatment of Poly-L-Lysine-Coated PLGA/Graphene Oxide Hybrid Fiber Matrices and Electrical Stimulation

Incorporation of Hydroxyapatite/Doxorubicin into the Chitosan/Polyvinyl Alcohol/Polyurethane Nanofibers for Controlled Release of Doxurubicin and Its Anticancer Property

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