Synergistic degradation of pyrene by five culturable bacteria in a mangrove sediment-derived bacterial consortium

Wanapaisan, Pagakrong; Laothamteep, Natthariga; Vejarano, Felipe; Chakraborty, Joydeep; Shintani, Masaki; Muangchinda, Chanokporn; Morita, Toyohiko; Suzuki-Minakuchi, Chiho; Inoue, Kengo; Nojiri, Hideaki; Pinyakong, Onruthai

doi:10.1016/j.jhazmat.2017.08.062

Cited by 147 publications

(59 citation statements)

References 45 publications

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“…Genetic and biochemical analyses about PAH-degrader of Gram-positive bacteria were also reported including thermophilic microorganisms [20,[26][27][28][29][30][31][32][33]. These facts imply that Gram-positive bacteria can play more important roles in the degradation of high molecular PAHs including biphenyl [34][35][36][37][38][39][40][41]. Further investigation including molecular analysis will provide us more clear information on naphthalene degradation including a lower pathway of thermophilic bacteria.…”

Section: Discussionmentioning

confidence: 94%

Isolation and Characterization of Genes Responsible for Naphthalene Degradation from Thermophilic Naphthalene Degrader, Geobacillus sp. JF8

et al. 2019

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Geobacillus sp. JF8 is a thermophilic biphenyl and naphthalene degrader. To identify the naphthalene degradation genes, cis-naphthalene dihydrodiol dehydrogenase was purified from naphthalene-grown cells, and its N-terminal amino acid sequence was determined. Using a DNA probe encoding the N-terminal region of the dehydrogenase, a 10-kb DNA fragment was isolated. Upstream of nahB, a gene for dehydrogenase, there were two open reading frames which were designated as nahAc and nahAd, respectively. The products of nahAc and nahAd were predicted to be alpha and beta subunit of ring-hydroxylating dioxygenases, respectively. Phylogenetic analysis of amino acid sequences of NahB indicated that it did not belong to the cis-dihydrodiol dehydrogenase group that includes those of classical naphthalene degradation pathways. Downstream of nahB, four open reading frames were found, and their products were predicted as meta-cleavage product hydrolase, monooxygenase, dehydrogenase, and gentisate 1,2-dioxygenase, respectively. A reverse transcriptase-PCR analysis showed that transcription of nahAcAd was induced by naphthalene. These findings indicate that we successfully identified genes involved in the upper pathway of naphthalene degradation from a thermophilic bacterium.

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Section: Discussionmentioning

confidence: 94%

Isolation and Characterization of Genes Responsible for Naphthalene Degradation from Thermophilic Naphthalene Degrader, Geobacillus sp. JF8

et al. 2019

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“…FW1 was successful in pyrene biodegradation. This cooperative interaction between bacterial strains led to enhanced pyrene biodegradation in the consortium [40]. Microbial consortium provides metabolic complementarity.…”

Section: Evaluation Of Growth Intensities and Petroleum Biodegradatiomentioning

confidence: 96%

“…APIO4 achieved the maximum benzene and diesel degradation. Wanapaisan et al [40] reported that the consortium formed by Mycobacterium spp. PO1 and PO2, Novosphingobium pentaromativorans PY1, Ochrobactrum sp.…”

Section: Evaluation Of Growth Intensities and Petroleum Biodegradatiomentioning

confidence: 99%

Comparison of petroleum biodegradation efficiencies of three different bacterial consortia determined in petroleum-contaminated waste mud pit

Özyürek

Bilkay

2020

SN Appl. Sci.

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The objectives of this study were to determine and compare the petroleum biodegradation efficiencies of three different bacterial consortia adapted to oil field. For this purpose, petroleum-contaminated waste mud sample was enriched in Bushnell Haas medium with 1% petroleum. Six different bacterial strains (four Gram-positive and two Gram-negative) were isolated from the enriched sample, and bacterial strains were identified with phenotypic and genotypic analyses. Each bacterial strain was evaluated in terms of biodegradation capacities by colorimetric analysis with the DCPIP redox indicator. Petroleum biodegradation efficiencies of three different consortia were investigated by gravimetric and GC-MS analyses. Consortium 1, consisting of Bacillus subtilis, Leuconostoc mesenteroides, Bacillus halotolerans, Enterococcus casseliflavus; consortium 2, consisting of Klebsiella pneumoniae and Pannonibacter phragmitetus; consortium 3, consisting of Bacillus subtilis, Leuconostoc mesenteroides, Bacillus halotolerans, Enterococcus casseliflavus, Klebsiella pneumoniae and Pannonibacter phragmitetus, were used in petroleum biodegradation. According to the results of gravimetric analysis, biodegradation of petroleum was 48%, 77% and 82% for consortium 1, consortium 2 and consortium 3, respectively. The results of GC-MS analysis revealed that removal of long-chain n-alkanes (C 23-C 32) was in the range of 35-58% by consortium 1; 56-67% by consortium 2; and 63-74% by consortium 3. It was emphasized that consortium 2 was more effective in biodegradation of petroleum than consortium 1. This study clearly demonstrated that consortium 3 is most effective in biodegradation of petroleum. This is assumed to be due to the synergistic effect between Gram-positive and Gram-negative bacterial strains. Furthermore, consortium 3 has been found to be very successful despite the shorter incubation period compared to other consortia in the literature. In this context, it is believed that the use of consortium 3 will contribute to the bioremediation of petroleum on a large scale.

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“…have been reported and their degradation pathways have subsequently been studied through chemical and omics analyses [23,[26][27][28]. Biodegradation by bacteria has been further advanced through the documentation of new isolates that represent diverse bacterial types that have been isolated from different environments and that possess different metabolic capabilities [18,29,30]. Therefore, in this work we carried out the isolation of indigenous bacteria from seawater and marine sediments samples through the enrichment method using pyrene as the sole carbon and energy source.…”

Section: Determination Of Pahs In Surface Seawater and Marine Sedimentsmentioning

confidence: 99%

Identification by MALDI-TOF MS of Environmental Bacteria with High Potential to Degrade Pyrene

Silva-Jiménez¹,

Araujo-Palomares²,

Macías-Zamora³

et al. 2018

J. Mex. Chem. Soc.

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An alternative to remove polycyclic aromatic hydrocarbons (PAHs) of the environment is using hydrocarbonoclastic bacteria. The aim of this work was to study the bacterial diversity of indigenous isolates with potential to degrade pyrene. We used MALDI-Biotyper (Bruker Daltonics) as a powerful analytical tool for identification. Bacteria were isolated from surface seawater and marine sediments samples of three sites from the coast of Rosarito Port, B.C., Mexico. Total concentration of PAHs in collected samples was quantified by GC-MS, showed values ranged 0.461-0.525 ng mL-1 and of 74 -266 ng g-1 in seawater and sediments samples, respectively. A total of 52 bacteria with capacity to grow in 25 mg L-1 pyrene as sole carbon and energy source were taxonomically identified and classified by MALDI-Biotyper system by comparing the mass spectra with library and/or to use chemometric tools as Principal Components Analysis (PCA) and Composite Correlation Index (CCI) to evaluate possible differences to isolate level. The identified isolates were represented by three phylogenetic groups: Firmicutes, Actinobacteria and Proteobacteria. Of these isolates, Kocuria strains appear to be excellent candidates to continue PAHs degradation studies.

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Synergistic degradation of pyrene by five culturable bacteria in a mangrove sediment-derived bacterial consortium

Cited by 147 publications

References 45 publications

Isolation and Characterization of Genes Responsible for Naphthalene Degradation from Thermophilic Naphthalene Degrader, Geobacillus sp. JF8

Isolation and Characterization of Genes Responsible for Naphthalene Degradation from Thermophilic Naphthalene Degrader, Geobacillus sp. JF8

Comparison of petroleum biodegradation efficiencies of three different bacterial consortia determined in petroleum-contaminated waste mud pit

Identification by MALDI-TOF MS of Environmental Bacteria with High Potential to Degrade Pyrene

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