Synergistic Cytotoxicity Effect by Combination of Methanol Extract of Parijoto Fruit (Medinilla speciosa Reinw. ex. Bl) and Cisplatin Against Hela Cell Line

Abstract

As one of the leading causes of death in worldwide, cervical cancer requires the effective therapies to reduce its mortality rate. One of the chemotherapy agents that frequently used in the treatment is cisplatin. However, due to drug resistance and its side effects, an agent that can be combined with cisplatin is needed. Parijoto fruit (Medinilla speciosa Reinw.ex.Bl) contains secondary metabolites compounds that have potential as anticancer. The study aims to determine the cytotoxic effect of methanol extract of Parijoto fruit calculated from the IC50 value and the synergicity of the combinational treatment with cisplatin evaluated from the Combination Index (CI) value and its cell viability by using MTT assay. Results showed that methanol extract of Parijoto fruit (MEP) performed cytotoxic effect on HeLa cell line with IC50 of 209.6 μg/mL while the value of IC50 of cisplatin against HeLa cells amounted to 12.8 μg/mL. The combination of 26.205 ppm (1/8 IC50) of MEP and 1.601 ppm (1/8 IC50) of Cisplatin performed synergistic effect on HeLa cell line with the CI value of 0.69. From the above results, it can be concluded that MEP is potential as co-chemotherapy agent based on the synergistic cytotoxicity effect with cisplatin.Keyword: cytotoxic, Medinilla speciosa, cisplatin, co-chemotherapy, MTT

Cellular Mechanism of the Cytotoxic Effects of Medinilla speciosa (Parijoto) Methanol Extract on MCF-7/HER-2 Cells is Through Increased Caspase-9 Protein and Decreased Vascular Endothelial Growth Factor Expression

“…This work is licensed under the Creative Commons Attribution 4.0 International License VEGF, circulating endothelial progenitor cells, inhibiting the enzymes of matrix metalloproteinases, which can degrade the basement membrane of blood vessels, inhibiting the proliferation, activation, and differentiation of endothelial cells. 4,16 Neoplastic cells release several pro-angiogenic factors, such as collagenase and plasminogen activators, that cause disruption of the basement membrane of the surrounding vasculature and trigger angiogenesis. In addition, these molecules act as chemotactic factors for endothelial cells and enhance the differentiation of the circulating bone marrow progenitor cells into endothelial cells.…”

“…The solution was then aseptically stirred and filtered using a 0.22 μm sterile microfilter in laminar airflow chamber, and its acidity was determined to be between 7.2-7.4. 16,18 To make 1 L of M199 medium for the Vero cell culture, the complete medium consisting of 10 mL of 10% FBS (Gibco), 3.7 g NaH2CO3, 2 g Hepes, 1.5 mL of 1.5% penicillin-streptomycin (Gibco), 0.5 mL of 0.5% fungizone (Gibco), 100 μg/mL hygromycin, and M199 (Gibco) was combined. Then, 1 L of distilled water was added.…”

“…Medinilla speciosa (Parijoto) is a type of shrub with red fruit, which has been empirically used as an enhancer of fertility in women, an anti-inflammatory, 15 and anticancer medication. 16 Secondary metabolites found in its fruits, including quercetindic flavonoids, tannin phenolic compounds, glycosides, and saponins, have been suggested to have antitumor properties. 17 Flavonoid and phenolic compounds are antioxidants that have an antitumor function.…”

POTENSI MINYAK NYAMPLUNG (Calophyllum inophyllum L.) DALAM SEDIAAN PELEMBAB KULIT DAN BIBIR

“…Persyaratan uji daya sebar untuk sediaan topikal adalah 5 -7 cm (Artanti et al, 2020) DAFTAR PUSTAKA…”

Application of TLC and UHPLC–QTOF–MS for the identification of aqueous two-phase extracted UV–fluorescent metabolites from Solanum retroflexum

“…Daji et al [2] investigated the phytochemical profile of Solanum retroflexum leaves using methanolic extracts and found a range of cinnamic acids, polyphenols and alkaloids. Though methanolic extraction has been applied quite extensively [2][3][4][5][6], this approach is accompanied by setbacks such as the use of toxic organic solvent which often requires large volumes for extraction solvents, making it a costly exercise. Hence, there is a dire need for effective, eco-friendly, single-step extraction techniques.…”