Synergistic curative effect of chicory extract and cisplatin against thioacetamide-induced hepatocellular carcinoma

Abstract

Aim: Hepatocellular carcinoma (HCC) is the dominant form of primary liver cancer and is histologically and etiologically distinct from other forms of primary liver cancer. The objective of this study was to elucidate the synergistic effect and the role of chicory extract [inulin (IN)] as a chemo-sensitizer for cisplatin (CIS) treatment of HCC. Methods: Five groups of rats were treated for 4 months. These groups consisted of the control group, a group receiving thioacetamide (TAA) (200 mg/kg b.w) in drinking water, a group injected intraperitoneally with a single dose of CIS (7.5 mg/kg b.w) in addition to TAA for 4 months, a group receiving oral doses of IN (10 mg/kg b.w) in addition to TAA for 4 months, and a group injected intraperitoneally with a single dose of CIS (7.5 mg/kg b.w) and IN (10 mg/kg b.w) plus TAA for 4 months. Results: The current data exhibited increment of serum and liver enzyme ( alanine aminotransferase, aspartate aminotransferase, alkaline phosphatase, creatine kinase, and lactate dehydrogenase) activity, serum lipid profi le levels (total lipids, total cholesterol, triglycerides, low-density lipoprotein, and very low-density lipoprotein), and a signifi cant increase in α-fetoprotein and bilirubin, accompanied with reduced total serum protein and albumin levels in a HCC rat model. Histopathologically, numerous alterations were detected in hepatic tissues of HCC rats, such as lymphocytic cell infi ltration, damage of hepatocytes, dilated congested central vein with degenerated endothelial cells, and congested blood sinusoids in addition to Masson's trichrome staining blue collagen fi bers in hepatocytes and central vein indicating hepatic fi brosis. Treatment of HCC rats with CIS or IN improved such deleterious effects, where IN is more effective than CIS, and the best effect can be observed in rats that received both CIS and IN. Conclusion: It could be concluded that IN in chicory extract acts as a chemo-sensitizer to CIS for treatment in an HCC rat model. Key words: Chicory; cisplatin; hepatocellular carcinoma; inulin INTRODUCTIONCancer is one of the most anxiety-inducing diseases and it is presenting with further prolongation and increasing occurrence.[1] The most common and recurrent primary malignancy of the liver is recognized as hepatocellular carcinoma (HCC), a major malignancy worldwide and is increasingly associated with cancer-related death.[2] HCC represents the most common primary malignancy of the liver. According to epidemiological surveys, the prevalence Synergistic curative effect of chicory extract and cisplatin Synergistic curative effect of chicory extract and cisplatin against thioacetamide-induced hepatocellular carcinoma against thioacetamide-induced hepatocellular carcinoma This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 3.0 License, which allows others to remix, tweak, and build upon the work non-commercially, as long as the author is credited and the new creations are licensed under the ...

Quantification of thioacetamide-induced liver necrosis using fractal analysis

“…sinusoids, due to loss of liver parenchyma. Previous studies on TAA-induced liver damage also showed prominent vascular alterations, such as sinusoidal dilatation and congestion and dilated and congested central vein (37,40) and are in accordance with the present study. Difference between Dsin values in the samples stained with Masson's trichrome showed higher values in the experimental, when compared to the control group.…”

Mechanistic evaluation of AMPK/SIRT1/FXR signaling axis, inflammation, and redox status in thioacetamide‐induced liver cirrhosis: The role ofCichorium intybuslinn (chicory)‐supplemented diet

“…Meanwhile, total bilirubin level was increase due to TAA‐induced oxidative stress and inflammation as conveyed by the current study. The results herein were in congruence with the preceding reports on TAA‐induced liver cell damage and cirrhosis (Hassan, Serag, Abdel‐Hamid, & Amr, ; Said, Said, Gameil, & Ammar, ; Shoeib et al, ). C. intybus linn supplementation improved liver cell synthetic and transport function, liver enzymes activities, and histopathological architecture, the condition that is in harmony with Hassan et al, .…”

“…Meanwhile, total bilirubin level was increase due to TAA-induced oxidative stress and inflammation as conveyed by the current study. The results herein were in congruence with the preceding reports on TAA-induced liver cell damage and cirrhosis(Hassan, Serag, Abdel- Hamid, & Amr, 2015;Said, Said, Gameil, & Ammar, 2013;Shoeib et al, 2018). C. intybus linn supplementation improved liver cell synthetic and transport function, liver enzymes activities, and histopathological architecture, the condition that is in harmony withHassan et al, 2015. Disturbed redox status as a result of oxidant/antioxidant imbalance causes oxidation of membrane lipid/proteins, DNA, structural and functional degeneration of cellular enzymes, and motivates proliferative/pro-inflammatory activity of HSCs nourishing the advance of liver fibrosis to cirrhosis…”

Effect of co-administration of Bee honey and some chemotherapeutic drugs on dissemination of hepatocellular carcinoma in rats

“…The anticancer effects of cisplatin, cyclophosphamide, and 5FU as chemotherapeutic drug used in the present study can easily related to the remarkable decrease of AFP as marker of HCC. This can be supported through related studies which found a decrease in AFP level after using these three drugs [[77], [78], [79], [80]].…”