Synergistic contribution of hydrosulfide and carbonate anions to the β-O-4 bond cleavage of lignin model compounds in a green liquor pretreatment for enzymatic hydrolysis of lignocellulosic materials

Gu, Feng; Posoknistakul, Pattaraporn; Shimizu, Soichi; Yokoyama, Tomoya; Jin, Yongcan; Matsumoto, Yuji

doi:10.1007/s10086-014-1411-5

Cited by 18 publications

(12 citation statements)

References 7 publications

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“…Currently, the process for the dissolving pulp comprises two distinct segments: the sulfite process and the prehydrolysis kraft process. For the latter process, water or steam prehydrolysis at 160–200 °C is carried out in a primary stage, resulting in partial hydrolysis of hemicelluloses and lignin degradation [4–6]. Constituents from biomass fractions can be dissolved in the prehydrolysis liquor, which is termed as prehydrolyzate.…”

Section: Introductionmentioning

confidence: 99%

A sustainable process for procuring biologically active fractions of high-purity xylooligosaccharides and water-soluble lignin from Moso bamboo prehydrolyzate

Huang

Wang

Liang³

et al. 2019

Biotechnol Biofuels

190

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Background Prehydrolyzate, which is from the prehydrolysis process in dissolving pulps industry, contains various sugar-derived and lignin compounds such as xylooligosaccharides (XOS), gluco-oligosaccharides, xylose, glucose, and soluble lignin (S-L). The XOS has several beneficial effects on human physiology. XOS and S-L in prehydrolyzate are difficult to efficiently fractionate due to their similar molecular weights and water solubility. In this work, we proposed a sustainable and green process using polystyrene divinylbenzene (PS-DVB) resin to simultaneously separate and recover XOS and S-L. Enzymatic hydrolysis with endo-1,4-β-xylanase and fermentation with P. stipites were sequentially applied to purify XOS to minimize xylose content as well as amplify contents of xylobiose and xylotriose. In addition, 2D-HSQC NMR was used to analyze the structural characteristics of XOS and S-L. Furthermore, the biological abilities of antioxidants and prebiotics of these fractions were investigated by scavenging radicals and cultivating intestinally beneficial bacterias, respectively. Results Results showed that PS-DVB resin could simultaneously separate XOS and solubilized lignin with excellent yields of 93.2% and 85.3%, respectively. The obtained XOS after being purified by enzymatic hydrolysis and fermentation contained 57.7% of xylobiose and xylotriose. 10.4% amount of inherent xylan was found in the S-L fraction obtained by PS-DVB resin separation. 2D-HSQC NMR revealed that lignin carbohydrate complexes existed in both XOS and S-L as covalent linkages between lignin and 4- O -methylglucuronoarabinoxylan. The biological application results showed that the antioxidant capacity of S-L was stronger than XOS, while XOS was superior in promoting growth of intestinal Bifidobacteria adolescentis and stimulating production of short-chain fatty acids by Lactobacillus acidophilus. Conclusions The proposed strategy of sequentially combining hydrophobic resin separation, enzymatic hydrolysis, and fermentation was successfully demonstrated and resulted in simultaneous production of high-quality XOS and solubilized lignin. These biomass-derived products in prehydrolyzate can be regarded as value-adding prebiotics and antioxidants. Electronic supplementary material The online version of this article (10.1186/s13068-019-1527-3) contains supplementary material, which is available to authorized users.

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Section: Introductionmentioning

confidence: 99%

A sustainable process for procuring biologically active fractions of high-purity xylooligosaccharides and water-soluble lignin from Moso bamboo prehydrolyzate

Huang

Wang

Liang³

et al. 2019

Biotechnol Biofuels

190

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“…CstA, is a protein involved in escaping from starvation during nutrient scavenging and transition to stationary-phase growth [ 34 ] and has been shown to be regulated by the cyclic AMP (cAMP)-cAMP receptor protein complex, a global regulator involved in sugar metabolism [ 35 ]. Although the specific role of CstA in the metabolism of aromatic compounds remains to be elucidated, it has been demonstrated that carbon starvation proteins are induced by different aromatic pollutants [ 36 ], suggesting its involvement as a mechanism of adaptive response to the presence of a more recalcitrant carbon source [ 37 ]. Similar to cstA , the gene encoding the competence protein F (Entcl_0322) was found to be upregulated in lignin-amended cultures after 7 hours.…”

Section: Resultsmentioning

confidence: 99%

Multi-time series RNA-seq analysis of Enterobacter lignolyticus SCF1 during growth in lignin-amended medium

et al. 2017

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The production of lignocellulosic-derived biofuels is a highly promising source of alternative energy, but it has been constrained by the lack of a microbial platform capable to efficiently degrade this recalcitrant material and cope with by-products that can be toxic to cells. Species that naturally grow in environments where carbon is mainly available as lignin are promising for finding new ways of removing the lignin that protects cellulose for improved conversion of lignin to fuel precursors. Enterobacter lignolyticus SCF1 is a facultative anaerobic Gammaproteobacteria isolated from tropical rain forest soil collected in El Yunque forest, Puerto Rico under anoxic growth conditions with lignin as sole carbon source. Whole transcriptome analysis of SCF1 during E.lignolyticus SCF1 lignin degradation was conducted on cells grown in the presence (0.1%, w/w) and the absence of lignin, where samples were taken at three different times during growth, beginning of exponential phase, mid-exponential phase and beginning of stationary phase. Lignin-amended cultures achieved twice the cell biomass as unamended cultures over three days, and in this time degraded 60% of lignin. Transcripts in early exponential phase reflected this accelerated growth. A complement of laccases, aryl-alcohol dehydrogenases, and peroxidases were most up-regulated in lignin amended conditions in mid-exponential and early stationary phases compared to unamended growth. The association of hydrogen production by way of the formate hydrogenlyase complex with lignin degradation suggests a possible value added to lignin degradation in the future.

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“…During GL pretreatment, the p -coumaric acid and ferulic acid units in grass lignin and α-O-4 linkages mostly associated with guaiacyl units in side chains are labile to cleave, resulting in a decreasing level of guaiacyl and p -hydroxyphenyl units in the residual lignin of pretreated biomass . Gu et al indicated that HS – exerts a synergistic effect with CO 3 2– to disrupt the more nonphenolic β-O-4 type in a GL solution than in other alkaline solutions . Consequently, GL pretreatment has better delignification selectivity as compared to SC pretreatment. , The resultant lignin obtained from GL pretreatment, however, faces the risk of sulfur contamination, which can complicate downstream conversion …”

Section: Impacts Of Alkali-based Pretreatment On Lignin Streammentioning

confidence: 99%

“…118 Gu et al indicated that HS − exerts a synergistic effect with CO 3 2− to disrupt the more nonphenolic β-O-4 type in a GL solution than in other alkaline solutions. 119 Consequently, GL pretreatment has better delignification selectivity as compared to SC pretreatment. 118,120 The resultant lignin obtained from GL pretreatment, however, faces the risk of sulfur contamination, which can complicate downstream conversion.…”

Section: Impacts Of Alkali-based Pretreatment Onmentioning

confidence: 99%

Alkali-Based Pretreatment-Facilitated Lignin Valorization: A Review

Zhang

Zhong

et al. 2020

Ind. Eng. Chem. Res.

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In an integrated biomass conversion process, pretreatment becomes an indispensable unit operation to enhance the accessibilities of cellulose and hemicelluloses for producing biofuels or chemicals. Besides carbohydrates utilization, lignin valorization is also considered as a key of achieving economic viability of biorefineries. The pretreatments using alkaline reagents exhibit a superior performance on boosting the yield of fermentable sugars by separating the lignin fraction and altering lignin structures and properties, offering the potential to facilitate the covalorization of lignin and carbohydrates. The diverse lignin recovered from alkali-based pretreatment affects the subsequent lignin depolymerization and conversion process due to the varying chemical and physical properties. In this review, the features and performances of different types of alkali-based pretreatments in the modifications to lignin are discussed. Also, the drill-down analysis of the promising methods to depolymerize lignin provides deep insights toward the conversion and upgrading of lignin isolated from alkaline pretreatment.

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Synergistic contribution of hydrosulfide and carbonate anions to the β-O-4 bond cleavage of lignin model compounds in a green liquor pretreatment for enzymatic hydrolysis of lignocellulosic materials

Cited by 18 publications

References 7 publications

A sustainable process for procuring biologically active fractions of high-purity xylooligosaccharides and water-soluble lignin from Moso bamboo prehydrolyzate

A sustainable process for procuring biologically active fractions of high-purity xylooligosaccharides and water-soluble lignin from Moso bamboo prehydrolyzate

Multi-time series RNA-seq analysis of Enterobacter lignolyticus SCF1 during growth in lignin-amended medium

Alkali-Based Pretreatment-Facilitated Lignin Valorization: A Review

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