Synergistic Bactericidal Effect of Chitosan/Zinc Oxide Based Nanocomposites Against Staphylococcus aureus

Abstract

<jats:p>The biocompatible Chitosan/Zinc oxide (CS/ZnO) nanocomposites (NCs) material was synthesized via a simple and versatile microwave assisted wet synthesis method. After the incorporation of CS in the ZnO nanoparticles (NPs), the crystalline structure of the modified NPs was retained in the NCs and it was clearly exposed in the X-ray diffraction (XRD) measurements. The Zeta potential measurement of CS/ZnO nanocomposites (NCs) shows more stability than ZnO NPs. The Field Emission Scanning Electron Microscopy (FE-SEM) measurements depict the formation of cauliflower like structure after the integration of CS in the ZnO NPs. The interaction between ZnO molecules in CS becomes more compact and is confirmed in the Fourier Transform Infrared Spectroscopy (FTIR) measurement. Bacterial activity was increased gradually with the CS/ZnO content and was analytically stronger against Gram-positive cells. This study has conclusively proved that reactive oxygen species (ROS) such as <jats:sup>•</jats:sup>OH, <jats:sup>•</jats:sup>O<jats:sup>2−</jats:sup> , and H<jats:sub>2</jats:sub>O<jats:sub>2</jats:sub> were significantly produced from aqueous suspension of CS/ZnO and were primarily responsible for the bactericidal activity.</jats:p>

A Streamlined Study on Chitosan-Zinc Oxide Nanomicelle Properties to Mitigate a Drug-Resistant Biofilm Protection Mechanism

“…However, there is still an ummet need to elucidate the physico-chemical properties of the nanomicellar CZNP that should ascertain its in vivo effectiveness on a broad spectrum of existing MDRs. While the effectiveness of micellar CZNPs has been previously been evidenced on a broad spectrum of some MDR pathogens (Limayem et al, 2016), some studies have confirmed the versatility of non-nanomicellar chitosanzinc oxide formulations to cope as different nanotherapies (Al-Naamani et al, 2016Al-Dhabaan et al, 2017;Baghaie et al, 2017;Chatterjee et al, 2017;Revathi and Thambidurai, 2018;Sathiya et al, 2018). This study herein will focus on the optimization of CZNPs nanotherapeutics in terms of physicochemical properties for nosocomial MDR biofilms, including elucidation of biofilm protection mechanisms such as EPS, which impact human and animal host cells in addition to agricultural systems and medical devices.…”

Combined effect of zinc oxide nano particle incorporated chitosan for better antimicrobial activity towards wound healing

“…-1 the present study MIC for S. aureus was 0.03 mg 100 µl , which may due to size variation of ZnO-NP. Similarly, Padmavathy and Vijayaraghavan (2008) prepared different size of nanoparticles and proved that nanoparticle sizes were inversely related to the Sathiya et al (2018) and they concluded that production of reactive oxygen species (ROS) would be a major reason for antibacterial activity. Similarly, Al-Naamani et al (2018) reported that higher reduction in bacterial and fungal was observed in ZnO-NP-CS coated package film thus extended the shelf life of Okra (Abelmoschus esculentus) for four more days.…”