Synergistic antioxidant activity of resveratrol with genistein in high-glucose treated Madin-Darby canine kidney epithelial cells

Chu, Chishih; Lu, Fung‐Jou; Yeh, Rang-Hui; Li, Zih‐Ling; Chen, Ching‐Hsein

doi:10.3892/br.2016.573

Cited by 10 publications

(4 citation statements)

References 16 publications

(13 reference statements)

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“…In LLC-PK1 cell line, cell survival was smaller for the same concentrations of l -arginine in comparison to MDCK I and II cell lines with peak of the effects shifted toward smaller concentrations of the antioxidant. These results are comparable to previous work done on kidney epithelial cell lines, which had shown dose dependence of antioxidant effect in reducing oxidative stress [ 34 , 35 ]. This shows that further studies are needed to determine what effect it would have on human kidney epithelial cells.…”

Section: Discussionsupporting

confidence: 91%

Antioxidant Pre-Treatment Reduces the Toxic Effects of Oxalate on Renal Epithelial Cells in a Cell Culture Model of Urolithiasis

Kizivat

Smolić

Marić

et al. 2017

IJERPH

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Urolithiasis is characterized by the formation and retention of solid crystals within the urinary tract. Kidney stones are mostly composed of calcium oxalate, which predominantly generates free radicals that are toxic to renal tubular cells. The aim of the study is to explore possible effects of antioxidant pre-treatment on inhibition of oxidative stress. Three cell lines were used as in vitro model of urolithiasis: MDCK I, MDCK II and LLC-PK1. Oxidative stress was induced by exposure of cells to sodium oxalate in concentration of 8 mM. In order to prevent oxidative stress, cells were pre-treated with three different concentrations of l-arginine and vitamin E. Oxidative stress was evaluated by determining the expression of superoxide dismutase (SOD), osteopontin (OPN), and by the concentration of glutathione (GSH). In all three cell lines, pre-treatment of antioxidants increased cell survival. Positive correlation of SOD and OPN expression as well as GSH concentration was observed in all groups of cells. Our results indicate that an antioxidant pre-treatment with l-arginine and vitamin E is able to hamper oxalate-induced oxidative stress in kidney epithelial cells and as such could play a role in prevention of urolithiasis.

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Section: Discussionsupporting

confidence: 91%

Antioxidant Pre-Treatment Reduces the Toxic Effects of Oxalate on Renal Epithelial Cells in a Cell Culture Model of Urolithiasis

Kizivat

Smolić

Marić

et al. 2017

IJERPH

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“…It is also present in win (Rafati et al, 2015). Resveratrol has been the focus of numerous studies investigating its biological attributes, which include mainly antioxidant activities (Chu et al, 2016). RSV is a polyphenolic compound that has demonstrated antiinflammatory and antioxidant effects, resulting from enhanced antioxidant enzymes production and modulating nuclear factors involved in the inflammation-oxidative stress cycle, as nuclear erythroid 2-related factor 2 (Nrf2) and nuclear factor-kB (NF-kB) (Saldanha et al, 2016).…”

Section: Introductionmentioning

confidence: 99%

Protective Effect of Resveratrol Against Morphine Damage to Kidneys of Mice

et al. 2017

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Morphine produces free radicals and cause apoptosis in some cell. Resveratrol (RSV) is a stilbenoid, a type of natural phenol, and a phytoalexin produced by several plants in response to injury. 48 male mice were randomly assigned to 8 groups. In this study, various doses of RSV (2, 8 and 20 mg/kg) and RSV plus Morphine (2, 8 and 20 mg/kg) were administered intraperitoneally to male mice for 20 consequent days and weight of kidneys, biochemical characteristics, morphometric markers and blood serum nitric oxide level were studied. The results indicated that morphine administration significantly increased the mean diameter of glomerulus and distal and proximal convoluted tubule, Lactate dehydrogenase (LDH), Blood urea nitrogen (BUN), creatinine and nitric oxide levels compared to the saline group (P<0.05). However, RSV and RSV plus morphine in all doses significantly decreased glomeruli number and LDH, BUN, creatinine and nitric oxide levels compared to morphine groups (p<0.05). Thus, it seems that resveratrol improved kidney damages induced by morphine in mice.

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“…In addition, the combination of RSV with genistein was reported to synergistically enhance antioxidant action in high-glucose treated Madin-Darby canine kidney (MDCK) epithelial cells when compared with RSV only and this combination may also have a therapeutic potential in diabetic nephropathy [141]. However, further studies are required to determine the effects of these combinations on the bioavailability of RSV and clinical relevance in diabetic conditions.…”

Section: The Way Forwardmentioning

confidence: 99%

Antidiabetic Effects of Resveratrol: The Way Forward in Its Clinical Utility

Oyenihi

Adeyanju

et al. 2016

Journal of Diabetes Research

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Despite recent advances in the understanding and management of diabetes mellitus, the prevalence of the disease is increasing unabatedly with resulting disabling and life-reducing consequences to the global human population. The limitations and side effects associated with current antidiabetic therapies have necessitated the search for novel therapeutic agents. Due to the multipathogenicity of diabetes mellitus, plant-derived compounds with proven multiple pharmacological actions have been postulated to “hold the key” in the search for an affordable, efficacious, and safer therapeutic agent in the treatment of the disease and associated complications. Resveratrol, a phytoalexin present in few plant species, has demonstrated beneficial antidiabetic effects in animals and humans through diverse mechanisms and multiple molecular targets. However, despite the enthusiasm and widespread successes achieved with the use of resveratrol in animal models of diabetes mellitus, there are extremely limited clinical data to confirm the antidiabetic qualities of resveratrol. This review presents an update on the mechanisms of action and protection of resveratrol in diabetes mellitus, highlights challenges in its clinical utility, and suggests the way forward in translating the promising preclinical data to a possible antidiabetic drug in the near future.

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Synergistic antioxidant activity of resveratrol with genistein in high-glucose treated Madin-Darby canine kidney epithelial cells

Cited by 10 publications

References 16 publications

Antioxidant Pre-Treatment Reduces the Toxic Effects of Oxalate on Renal Epithelial Cells in a Cell Culture Model of Urolithiasis

Antioxidant Pre-Treatment Reduces the Toxic Effects of Oxalate on Renal Epithelial Cells in a Cell Culture Model of Urolithiasis

Protective Effect of Resveratrol Against Morphine Damage to Kidneys of Mice

Antidiabetic Effects of Resveratrol: The Way Forward in Its Clinical Utility

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