Synergistic Antioxidant Activity of Honey Bee Products and Their Mixtures

Abstract

The goal of this study was to assess the impact of binary combination antioxidant synergistic effects of honeybee products (citrus honey, clover honey, sugar feeding honey, bee pollen, bee bread, bee wax, old wax comb, Egyptian propolis, Chinese propolis, royal jelly, Drone brood homogenate, worker brood homogenate, queen brood homogenate, bee venom) and The present study compared the antioxidant activity between ethanol and water extracts of bee products and evaluated the synergistic antioxidant activity effect of binary combination of bee products (water and ethanol extracts, separately), the antioxidant activity was analyzed via DPPH radical scavenging activity assay and found propolis as one of the most powerful antioxidant among all the honeybee products examined, and the ethanol (80%) extraction method recorded more antioxidant activity than the water extract, but in the royal jelly, drone brood homogenate, worker brood homogenate, queen brood homogenate and bee venom the water extract were the highest. The obtained results of honey bee product mixture activity affected by the interaction between chemical compositions of them, which had an impact on their antioxidant activity. And several of these binary combinations showed synergistic results; this might be because adding more antioxidant components from other products increased the antioxidant capacity.