Synergistic Anticancer Potential of Water Garlic Extract and Copper in a Human Hepatocarcinoma Cell Line

Martino, Angelo; Piera, Torricelli; Abu-Zeid, Hanan Mahmoud; Шевченко, Анна Николаевна; Alberto, Siciliano; Beninati, Simone

doi:10.11648/j.crj.20160402.11

Cited by 3 publications

(4 citation statements)

References 19 publications

(11 reference statements)

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“…In our experiments high concentrations of garlic extract definitely contains a lot of allicin, which could be the explanation of the lack of caspase-3 activity. Similar to our results, De Martino et al (2016) showed that the aqueous garlic extract, supplemented with copper, enhanced anti-proliferative and a caspase-independent apoptotic activity in HepG2 cancer cell line (De Martino et al, 2016).…”

Section: Discussionsupporting

confidence: 90%

“…However, authors show that in each of studied cell lines different concentrations of garlic extracts stimulated caspase-3 activity. Moreover, it has also been demonstrated that garlic extracts or garlic components such as allicin or gallic acid cause a caspase-independent cell death (De Martino et al, 2016, Ji et al, 2009, Park et al, 2005).…”

Section: Introductionmentioning

confidence: 99%

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Cytotoxic effects of two extracts from garlic (Allium sativum L.) cultivars on the human squamous carcinoma cell line SCC-15

Szychowski

Binduga

Rybczyńska‐Tkaczyk

et al. 2018

Saudi Journal of Biological Sciences

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Garlic (Allium sativum L., Alliaceae) has acquired a reputation as a therapeutic agent and herbal remedy to prevent and treat several pathologies. The aim of the present study was to determine the effect of two Allium sativum L. cultivars, Harnaś and Morado, on reactive oxygen species (ROS) production, viability and apoptotic process in human squamous carcinoma cell line SCC-15. The experiments were conducted on SCC-15 cell line exposed to increasing concentrations of garlic extracts of 0.062, 0.125, 0.250, 0.500 and 1.000 mg/mL. After the experiments, ROS formation, caspase-3 activity and neutral red uptake were measured in the cells, and in a collected medium lactate dehydrogenase (LDH) release was measured. The Spanish cultivar Morado has demonstrated higher potential to stimulate ROS production in SCC-15 cells after a short time period (6 h) than the Polish cultivar Harnaś. However, the Polish cultivar Harnaś manifested more prolonged potential to stimulate ROS production in SCC-15 cells. Both studied garlic extracts induced cytotoxicity on SCC-15 cell line which was probably ROS-dependent. We also determined that in SCC-15 cells high concentrations of studied extracts did not cause activation of caspase-3 which suggested caspase-independent or necrotic cell death.

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Section: Discussionsupporting

confidence: 90%

Section: Introductionmentioning

confidence: 99%

Cytotoxic effects of two extracts from garlic (Allium sativum L.) cultivars on the human squamous carcinoma cell line SCC-15

Szychowski

Binduga

Rybczyńska‐Tkaczyk

et al. 2018

Saudi Journal of Biological Sciences

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“…Antiproliferative effect, overexpression of p53 and p21 (break of DNA strand) [29] Crude garlic Inhibition of cell proliferation [137] Heat-aging Inhibition of cell proliferation [7] SGC-7901 (human gastric cancer) Aged black garlic Inhibition of cell growth through apoptosis, inhibition of tumor growth in rats, which may result from antioxidant and immunomodulating effects [141] AsPC-1 (pancreatic beta cells) Oil…”

Section: Aqueousmentioning

confidence: 99%

Methods of Isolation of Active Substances from Garlic (Allium sativum L.) and Its Impact on the Composition and Biological Properties of Garlic Extracts

2022

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Garlic (Allium sativum L.) is widely used in the human diet and in scientific research due to its biological properties. Various factors, e.g., temperature, pressure, extraction method, type of solvent, size, and territorial origin of garlic, affect the amount and type of bioactive compounds obtained from garlic extracts. In turn, the content of bioactive compounds correlates with the biological activity of the extracts. Therefore, the aim of this review was to summarize the current state of knowledge of the methods and effectiveness of isolation of active substances from garlic and their impact on the garlic extract composition and, consequently, biological properties. According to the literature, extracts obtained using water as a solvent are mainly responsible for antimicrobial properties, which is related to, inter alia, the high content of allicin. The use of alcohols, such as methanol or ethanol, is associated with the outstanding antioxidant power of extracts resulting from the presence of phenolic compounds. In turn, due to the presence of diallyl disulfide and disulfide trisulfide, garlic oil has anticancer potential. Acetone is the most effective organic solvent; however, it is not suitable for immediate consumption.

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“…In a previous paper [1] we reported that water-soluble garlic extracts (GE), supplemented with copper (Cu), exerts an enhanced anti-proliferative and a caspase-independent apoptotic activity in a human hepatocarcinoma HepG2 cancer cell line. The bioavailability of garlic compounds depends on several factors, first of all the method of processing the bulbs to obtain the garlic extract.…”

Section: Introductionmentioning

confidence: 99%

Copper Enhances Antitumor Activity of Water Soluble Garlic Extract Components on a Human Hepatoma Cell Line

Martino¹

2016

CRJ

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Water garlic extract combined with Copper (GE-Cu) shows specific anti-proliferative and antitumoral activity in human hepatocarcinoma (HepG2) cancer cell line, while it is not active on differentiated or primary isolated cells, normal human dermal fibroblasts (HDF) and human epidermal keratinocytes (HEK). Since other transition metals do not affect cell viability, the observed antineoplastic property, was found specific for copper (Cu), and atomic absorption analyses demonstrate an accumulation of this metal inside the cell nuclei. GE-Cu treatment of HepG2a cancer cells, induces cell differentiation, growth arrest and programmed cell death. Furthermore, GE-Cu produces a continuous and prolonged flux of oxidative radicals, which down-regulates nuclear antioxidant defenses and repair systems, thus generating direct double-strand breaks to DNA. Indeed, it was observed that in GE-Cu-treated HepG2 cancer cells, histone deacetylase (HDAC) is inhibited, and histone H2AX is early phospho-activated. DNA double strand break downstream, likely responsible for the observed cell death in HepG2 cell, was evidenced by an early over expression of p53 and p21. JNK/c-Jun and p-38 phosphorylative cascade are activated as response to oxidative stress also. The resulting apoptosis appears to be caspases-independent because, under our experimental conditions, nuclear translocation of the apoptosis inducing factor (AIF) is operative. Our data suggest that GE-Cu possess an active specific antitumor capability, which could provide valuable new tools in cancer prevention and in supporting traditional chemotherapy.

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Synergistic Anticancer Potential of Water Garlic Extract and Copper in a Human Hepatocarcinoma Cell Line

Cited by 3 publications

References 19 publications

Cytotoxic effects of two extracts from garlic (Allium sativum L.) cultivars on the human squamous carcinoma cell line SCC-15

Cytotoxic effects of two extracts from garlic (Allium sativum L.) cultivars on the human squamous carcinoma cell line SCC-15

Methods of Isolation of Active Substances from Garlic (Allium sativum L.) and Its Impact on the Composition and Biological Properties of Garlic Extracts

Copper Enhances Antitumor Activity of Water Soluble Garlic Extract Components on a Human Hepatoma Cell Line

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