Synergistic antibacterial efficacies of the combination of bovine lactoferrin or its hydrolysate with probiotic secretion in curbing the growth of meticillin-resistant Staphylococcus aureus

Chen, Po−Wen; Jheng, Trista Tingyun; Shyu, Ching-Lin; Mao, Frank Chiahung

doi:10.1099/jmm.0.052639-0

Cited by 26 publications

(24 citation statements)

References 43 publications

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“…(registered in a strain dependent-manner) was herein reported at HLF concentrations ranging from 5 to 20 mg/mL; a bactericidal effect was also registered for the selected S. epidermidis strains at 10 mg/mL. Results agreed with the HLF growth-inhibitory effects previously reported against methicillin-resistant S. aureus which registered 8 < MIC values < 64 mg/mL [50]. To the best of our knowledge, no data were instead reported against CoN strains.…”

Section: Discussionsupporting

confidence: 89%

Lactoferrin-Derived Peptides as a Control Strategy against Skinborne Staphylococcal Biofilms

et al. 2020

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Coagulase-negative staphylococci (CoNS) widely colonize the human skin and play an active role in host defense. However, these bacteria may cause malodours and increase infection incidence rate in immune-compromised patients and individuals with catheters and implants. CoNS spreading is favored by biofilm formation that also promotes the release of virulence factors and drug resistance. Biofilm control or eradication by antimicrobial peptides (AMPs) represents an attractive strategy which is worth investigating. In this work, bovine lactoferrin (BLF) hydrolysate (HLF) was in vitro evaluated for its antimicrobial and antibiofilm activities against skin-related coagulase negative and positive staphylococci. Despite a minimal inhibitory concentration (MIC) recorded for HLF ranging from 10 to more than 20 mg/mL, a minimal biofilm inhibitory concentration (MIBC) equal to 2.5 mg/mL was found for most target strains. Conversely, MIBC values referred to the individual peptides, LFcinB or LFmpin (herein purified and identified) were significantly lower. Finally, the application of 2.5 mg/mL HLF solution by dipping and spraying on biofilm-attached glass surfaces also caused a high biofilm eradication rate depending on the incubation time, thus attracting interest for future applications in cosmetic formulation for skin care.

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Section: Discussionsupporting

confidence: 89%

Lactoferrin-Derived Peptides as a Control Strategy against Skinborne Staphylococcal Biofilms

et al. 2020

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“…reuteri or L . fermentum cultures enhanced the inhibitory activity of apobLf against pathogenic strains and even against methicillin-resistant Staphylococcus (S.) aureus (MRSA) without affecting lactobacilli growth [74,75]. A presumable mechanism involves the ability of apoLf to facilitate the entrance of the secreted antimicrobial compounds released by the probiotic strains in the culture supernatant within the MRSA bacteria [75].…”

Section: Lactoferrin: Effects On Probiotic Growthmentioning

confidence: 99%

“…Culture assays in bifidobacteria and lactobacilli strains showed that bacterial growth was either inhibited or unaltered by the hydrolysate of apobLf obtained by treatment with pepsin [73]. Moreover, compared with the full-length protein, the apobLf hydrolysate displayed strong inhibitory action on the growth of foodborne pathogens and MRSA [73,75]. Combining culture supernatants from apobLf-resistant probiotic strains with apobLf hydrolysate resulted in synergistic inhibitory action against foodborne pathogens and MRSA [73,75].…”

Section: Lactoferrin: Effects On Probiotic Growthmentioning

confidence: 99%

“…Moreover, compared with the full-length protein, the apobLf hydrolysate displayed strong inhibitory action on the growth of foodborne pathogens and MRSA [73,75]. Combining culture supernatants from apobLf-resistant probiotic strains with apobLf hydrolysate resulted in synergistic inhibitory action against foodborne pathogens and MRSA [73,75]. These results may reflect the role of the synergistic antimicrobial effect of bLf hydrolysate and probiotic strains resistant to bLf hydrolysate in the intestinal milieu.…”

Section: Lactoferrin: Effects On Probiotic Growthmentioning

confidence: 99%

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The Impact of Lactoferrin on the Growth of Intestinal Inhabitant Bacteria

Vega-Bautista

Garza

Carrero

et al. 2019

IJMS

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Lactoferrin (Lf) is an iron-binding milk glycoprotein that promotes the growth of selected probiotic strains. The effect of Lf on the growth and diversification of intestinal microbiota may have an impact on several issues, including (i) strengthening the permeability of the epithelial cell monolayer, (ii) favoring the microbial antagonism that discourages the colonization and proliferation of enteric pathogens, (iii) enhancing the growth and maturation of cell-monolayer components and gut nerve fibers, and (iv) providing signals to balance the anti- and pro-inflammatory responses resulting in gut homeostasis. Given the beneficial role of probiotics, this contribution aims to review the current properties of bovine and human Lf and their derivatives in in vitro probiotic growth and Lf interplay with microbiota described in the piglet model. By using Lf as a component in pharmacological products, we may enable novel strategies that promote probiotic growth while conferring antimicrobial activity against multidrug-resistant microorganisms that cause life-threatening diseases, especially in neonates.

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“…Together, these findings suggest an enhanced ability of CC5 to endure selective pressures and colonize the cornea and adjacent tissues. Lactoferrin, one the most abundant tear proteins with antimicrobial activity is inhibitory for S. aureus, including MRSA, but this activity varies among clinical isolates from different sites of isolation (47,48). In one study, clinical S. aureus isolates causing bloodstream infections (50%) were frequently resistant to lactoferrin concentrations ≥ 20 µM, while isolates from conjunctivitis (33%) and SSTI (13%) were less often resistant (47).…”

Section: Discussionmentioning

confidence: 99%

Hospital-Associated Multidrug-Resistant MRSA Lineages Are Trophic to the Ocular Surface and Cause Severe Microbial Keratitis

Bispo

Ung

Chodosh

et al. 2020

Front. Public Health

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Methicillin-resistant Staphylococcus aureus (MRSA) is a common cause of severe and difficult to treat ocular infection. In this study, the population structure of 68 ocular MRSA isolates collected at Massachusetts Eye and Ear between January 2014 and June 2016 was assessed. By using a combination of multilocus sequence typing (MLST) analysis, SCCmec typing and detection of the panton-valentine leukocidin (PVL) gene, we found that the population structure of ocular MRSA is composed of lineages with community and hospital origins. As determined by eBURST analysis of MLST data, the ocular MRSA population consisted of 14 different sequence types (STs) that grouped within two predominant clonal complexes: CC8 (47.0%) and CC5 (41.2%). Most CC8 strains were ST8, harbored type IV SCCmec and were positive for the PVL-toxin (93.7%). The CC5 group was divided between strains carrying SCCmec type II (71.4%) and SCCmec type IV (28.6%). Remaining isolates grouped in 6 different clonal complexes with 3 isolates in CC6 and the other clonal complexes being represented by a single isolate. Interestingly, major MRSA CC5 and CC8 lineages were isolated from discrete ocular niches. Orbital and preseptal abscess/cellulitis were predominantly caused by CC8-SCCmec IV PVL-positive strains. In contrast, infections of the cornea, conjunctiva and lacrimal system were associated with the MDR CC5 lineage, particularly as causes of severe infectious keratitis. This niche specialization of MRSA is consistent with a model where CC8-SCCmec IV PVL-positive strains are better adapted to cause infections of the keratinized and soft adnexal eye tissues, whereas MDR CC5 appear to have greater ability in overcoming innate defense mechanisms of the wet epithelium of the ocular surface.

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Synergistic antibacterial efficacies of the combination of bovine lactoferrin or its hydrolysate with probiotic secretion in curbing the growth of meticillin-resistant Staphylococcus aureus

Cited by 26 publications

References 43 publications

Lactoferrin-Derived Peptides as a Control Strategy against Skinborne Staphylococcal Biofilms

Lactoferrin-Derived Peptides as a Control Strategy against Skinborne Staphylococcal Biofilms

The Impact of Lactoferrin on the Growth of Intestinal Inhabitant Bacteria

Hospital-Associated Multidrug-Resistant MRSA Lineages Are Trophic to the Ocular Surface and Cause Severe Microbial Keratitis

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