Synergistic Activity of Lupinifolin in Combinations with Antibiotics Against Staphylococcus aureus

Rattanakiat, Sakulrat; Kaewchang, Kanladar; Thongsang, Sofia; Jaruchotikamol, Achida; Pulbutr, Pawitra

doi:10.3923/pjbs.2021.656.662

Cited by 5 publications

(12 citation statements)

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“…This indicated the synergistic effects of lupinifolin when used in combinations with these two penicillins. However, lupinifolin caused an indifferent action when combined with vancomycin with an FIC index of 0.75 14 . The combinations of lupinifolin and ampicillin, cloxacillin or vancomycin at their sub-MICs produced significant inhibitory actions against MRSA biofilm formation in this study.…”

Section: Inhibitory Activities Of Lupinifolin In Combinations Withmentioning

confidence: 95%

“…Therefore, lupinifolin operates a distinct antibacterial mechanism of action from that of cell wall synthesis inhibitors, including β-lactam antibiotics and vancomycin, which are primarily used for the treatment of MSSA and MRSA infections. The previous study recently reported that the combinations of lupinifolin with ampicillin or cloxacillin produced synergistic effects against MSSA and MRSA with a FICI of <0.5 14 . Lupinifolin at its sub-MICs significantly exhibited inhibitory actions against biofilm formation of S. mutans, E. faecalis, E. faecium and MSSA 11,13 .…”

Section: Derris Reticulatamentioning

confidence: 96%

“…(Leguminosae-Papilionoideae). It has been demonstrated that lupinifolin possesses antibacterial activity against certain gram-positive cocci including Streptococcus mutans, Enterococcus faecalis, Enterococcus faecium, MSSA as well as MRSA [7][8][9][10][11][12][13][14] . Lupinifolin was found to exert its antibacterial activity via disruption of bacterial cell membrane structure and functions [9][10] .…”

Section: Derris Reticulatamentioning

confidence: 99%

“…A modified micro broth dilution method was performed according to Clinical and Laboratory Standards Institute (CLSI) guidelines 14 . Stock solutions of lupinifolin, ampicillin (Sigma-Aldrich®, A8351), cloxacillin (Sigma-Aldrich®, C9393) and vancomycin (Sigma-Aldrich®, V2002) were prepared in 2-fold serial dilutions in their respective vehicles (0.1 M NaOH for lupinifolin and sterile deionized water for ampicillin, cloxacillin and vancomycin).…”

Section: Determination Of the Micmentioning

confidence: 99%

“…From our previous study, it was demonstrated that lupinifolin can potentiate the antibacterial activity of ampicillin and cloxacillin against MRSA with the FIC indices of <0.5625 and <0.5156 respectively 14 . This indicated the synergistic effects of lupinifolin when used in combinations with these two penicillins.…”

Section: Inhibitory Activities Of Lupinifolin In Combinations Withmentioning

confidence: 99%

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Antibiofilm Formation Activity of Lupinifolin Against Methicillin-Resistant Staphylococcus aureus

Rattanakia¹,

Taensantia²,

Jaemamporn³

et al. 2022

Pakistan J. of Biological Sciences

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Background and Objective: Biofilm formation activity of Methicillin-resistant Staphylococcus aureus (MRSA) is one of the crucial factors rendering this pathogenic bacterium difficult to be eradicated. It has been reported that lupinifolin, is a major phytochemical agent isolated from Derris reticulata Craib. stem possesses antibacterial activity against MRSA. This study aimed to investigate the effects of lupinifolin and its combinations with some antibacterial drugs, including ampicillin, cloxacillin or vancomycin, on the biofilm formation activity of MRSA. Materials and Methods: The crystal violet biofilm formation assay was performed to evaluate the biofilm formation activity. Results: Lupinifolin produced a significant inhibitory activity against MRSA biofilm formation with the median inhibitory concentration (IC 50 ) of 7.96±3.05 µg mLG 1 (n = 6) at 24 hrs incubation. Lupinifolin at the concentrations of sub-MICs (1, 2, 4 and 8 µg mLG 1 ) combined with the antibacterial drugs at their sub-MICs also exhibited substantial antibiofilm formation activities. The maximal antibiofilm activity was found with the combination of lupinifolin (8 µg mLG 1 ) and vancomycin (1 µg mLG 1 ) by the percentage inhibition of 102.39±0.89 (n = 8). The antibiofilm formation activities of the combinations between lupinifolin and the antibacterial drugs at various concentrations tested were also significantly higher than those of lupinifolin alone. Conclusion: These results indicated that lupinifolin can potentially be developed as an antibacterial enhancer for the management of biofilm-associated bacterial infections caused by MRSA, in which the current pharmacological treatment is still limited.

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Section: Inhibitory Activities Of Lupinifolin In Combinations Withmentioning

confidence: 95%

Section: Derris Reticulatamentioning

confidence: 96%

Section: Derris Reticulatamentioning

confidence: 99%

Section: Determination Of the Micmentioning

confidence: 99%

Section: Inhibitory Activities Of Lupinifolin In Combinations Withmentioning

confidence: 99%

See 3 more Smart Citations

Antibiofilm Formation Activity of Lupinifolin Against Methicillin-Resistant Staphylococcus aureus

Rattanakia¹,

Taensantia²,

Jaemamporn³

et al. 2022

Pakistan J. of Biological Sciences

Self Cite

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Antimycobacterial Precatorin A Flavonoid Displays Antibiofilm Activity against Mycobacterium bovis BCG

Niño-Padilla,

Espitia,

Velazquez

et al. 2023

ACS Omega

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The aim of this study was to evaluate the potential antibiofilm activity of Rhynchosia precatoria (R. precatoria) compounds over Mycobacterium bovis BCG (M. bovis BCG) as a model for Mycobacterium tuberculosis (Mtb). We evaluated the antibiofilm activity as the ability to both inhibit biofilm formation and disrupt preformed biofilms (bactericidal) of R. precatoria compounds, which have been previously described as being antimycobacterials against Mtb. M. bovis BCG developed air−liquid interface biofilms with surface attachment ability and drug tolerance. Of the R. precatoria extracts and compounds that were tested, precatorin A (PreA) displayed the best biofilm inhibitory activity, as evaluated by biofilm biomass quantification, viable cell count, and confocal and atomic force microscopy procedures. Furthermore, its combination with isoniazid at subinhibitory concentrations inhibited M. bovis BCG biofilm formation. Nonetheless, neither PreA nor the extract showed bactericidal effects. PreA is the R. precatoria compound responsible for biofilm inhibitory activity against M. bovis BCG.

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Flavonoids effects against bacteria associated to periodontal disease and dental caries: a scoping review

Carneiro,

de Castro,

Benetti

et al. 2024

Biofouling

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Synergistic Activity of Lupinifolin in Combinations with Antibiotics Against Staphylococcus aureus

Cited by 5 publications

References 0 publications

Antibiofilm Formation Activity of Lupinifolin Against Methicillin-Resistant Staphylococcus aureus

Antibiofilm Formation Activity of Lupinifolin Against Methicillin-Resistant Staphylococcus aureus

Antimycobacterial Precatorin A Flavonoid Displays Antibiofilm Activity against Mycobacterium bovis BCG

Flavonoids effects against bacteria associated to periodontal disease and dental caries: a scoping review

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