Synergistic activity of chlorhexidine and synoeca‐MP peptide against <i>Pseudomonas aeruginosa</i>

Dantas, Elaine M. G. L.; Lima, Stella M. F.; Cantuária, Ana Paula de Castro; Amorim, Ingrid Aquino; Almeida, Jeeser Alves de; Cunha, Tássio F.; Franco, Octávio Luiz; Rezende, Taia Maria Berto

doi:10.1002/jcp.28265

Cited by 8 publications

(10 citation statements)

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“…Among other mastoparans, synoeca‐MP is a promising therapeutic for the treatment of diseases caused by resistant pathogens. [ 19 ] Our current data demonstrate the robustness and importance of biophysical studies for understanding the structure–function relationship of AMPs. The atomically resolved structure and orientation of synoeca‐MP in anionic SDS micelles can be used to understand the mode of action in the lipid membrane and to advance the development of more effective analogs.…”

Section: Discussionmentioning

confidence: 66%

“…Currently, synoeca‐MP has been shown to exhibit significant activity against bacterial and fungal strains in antimicrobial assays in vitro . [ 18 ] Another step in the analysis of peptide activity was taken by Dantas et al ., [ 19 ] who analyzed the synergistic effect of the peptide synoeca‐MP together with chlorhexidine against biofilms of Pseudomonas aeruginosa . The study shows great results for a potential oral infection treatment by combining both molecules.…”

Section: Discussionmentioning

confidence: 99%

“…[ 18 ] Synoeca‐MP has already been tested in synergy with other drugs such as chlorhexidine and levofloxacin, which increased its activity against Pseudomonas aeruginosa and Klebsiella pneumoniae , respectively. [ 19,20 ] Regarding immune cytokines, synoeca‐MP has been shown to stimulate the production of pro‐inflammatory cytokines, such as TNF‐α and nitric oxide. [ 20 ] Due to its low hemolytic and cytotoxic activities, several biotechnological applications have been used to obtain efficient and cost‐effective approaches for the recombinant production and purification of this peptide.…”

Section: Introductionmentioning

confidence: 99%

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Synoeca‐MP: New insights into its mechanism of action by using NMR and molecular dynamics simulations approach

et al. 2022

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Synoeca-MP is a 14-residue amidated peptide, belongs to the mastoparan family and it is found in the venom of the wasp Synoeca surinama and has antibacterial and antifungal activity. The low cytotoxicity of the peptide also makes it an excellent candidate for drug development. To better understand its selectivity and interaction with the membrane, the peptide behavior in membrane-like environments was studied here and the peptide structure in SDS micelles was determined by NMR spectroscopy. The behavior of the peptide in hydrophobic media and in different pH ranges was studied by CD spectroscopy. The incorporation of residues into the anionic micelles was studied by hydrogen-deuterium exchange. The peptide stability and insertion in the micelles was studied by molecular dynamics simulations. Synoeca-MP, bound to SDS micelles, exhibits a partial α-helix conformation, with the first five residues and the last two unfolded. H/D exchange showed that the peptide has a slow exchange rate. After 164 h, four residues had not yet completed H/D substitution, suggesting parallel alignment of the peptide with the micelle, mainly due to the hydrophobic interface. This may indicate a carpet interaction model of the peptide with micelles. The molecular simulation study of peptide showed that the peptide consists of a well-folded alpha-helix core and unfolded extremities, which are responsible for the nature of the peptide interaction. The biophysical analyses can improve

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Section: Discussionmentioning

confidence: 66%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Synoeca‐MP: New insights into its mechanism of action by using NMR and molecular dynamics simulations approach

et al. 2022

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“…The venom of A pallens contains a great variety of proteins with different biological functions, including compounds that are neurotoxins, proteins of the type 6 lectin that have biological activity against pathogens, toxic peptides, and proteins with cytolytic and proteolytic action (Mendonça et al, 2019). Synoeca surinama venom showed potential antibacterial activity against Grampositive and Gram-negative bacteria which was associated with the presence of antimicrobial peptides such as synoeca-MP (Dantas et al, 2019;Freire et al, 2020;Mortari et al, 2012). Also, venom from Protopolybia exigua exhibited antimicrobial activity due to the mastoparan peptides it contains (Mendes et al, 2005;Murata et al, 2009).…”

Section: Vespidaementioning

confidence: 99%

Insects as sources of food and bioproducts: a review from Colombia

Pérez-Grisales

Soto

2022

JoBAZ

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Background Insects are known to be important sources of food and bioproducts, and companies around the world are currently offering goods and services based on their production and use. Colombia is one of the richest countries in the world in terms of biodiversity, with a great variety of insects that are not exploited for these uses at this time. Most studies relating to insects in Colombia are focused on agricultural pests or disease transmitters, and in most cases the advantages and potential applications of insects in the areas of agro-industry, medicine, biotechnology, and food are poorly known. To recognize the native species previously considered as a source of bioproducts, it is necessary to better evaluate their potential uses, as well as the possibilities of innovating with products derived from them. It is also important to consider advantages and disadvantages of using insects for specific purposes, minimum quality requirements and national and international regulations for production and marketing. Main body The growing world population has led to an increase in the demand for food and animal products, increasing the need for animal production. This has resulted in high pressure on the environment, water resources and biodiversity, which also contribute to climate change. New strategies are required, and emerging solutions include the use of alternative sources for bioproducts or meat, changing diets, and migrating to sustainable production systems. In the present study, available information pertaining to 107 species of insects reported in Colombia that have been indicated to be sources to produce bioproducts, or that are currently being used for that purpose is revised and analyzed. The insects documented are from 67 genera and mainly include the orders Hymenoptera (59%), Coleoptera (10%), and Blattaria (11%). Seventy-one percent (71%) of the insect species included are important as foods or food supplements, with 9% related to established or commercial products currently in development; and 36% currently recognized for their importance in obtaining valuable non-edible bioproducts within the pharmaceutical industry, medicine, biotechnology, and agro-inputs sectors. A list of species is presented and uses and applications are discussed. Conclusions Despite Colombia's enormous potential for sustainable development of insect-derived products, there is a lack of studies in this area. Most of the insects reported in this work are related to local and traditional knowledge and folk medicine of some populations in the country. In addition to apiculture, there are not industrialized insect farms in Colombia; however, there have been some initiatives to produce crickets of the species Acheta domestica and mealworms Tenebrio mollitor for human and animal consumption. Recently the traditional consumption of ants and certain termite species in some areas of the country has been refreshed by some chefs experimenting with insects in gourmet restaurants. There are few studies on the nutritional value or pharmaceutical uses of the local species and there is no clear regulation for breeding or use. This highlights the need for in-depth study and discussion of the advantages and disadvantages for potential use in the country.

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“…However, the concentrations of α-defensin found in the intestinal crypts or in neutrophil granules are sufficient to have a bactericidal effect even in the salinity conditions mentioned above. θ-defensins and hBD -3 maintain their bactericidal activity in conditions of physiological salinity, in addition they have antiviral activity (anti-HIV) [27].…”

Section: General Properties / Characteristics Of Antimicrobial Peptidesmentioning

confidence: 99%

Properties / Characteristics of Antimicrobial Peptides

Varga¹

2022

J Med Healthcare

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Antimicrobial peptides (AMPs) are natural antibiotics, synthesized by ribosomes, with an effect on the natural immunity of animal organisms. AMPs differ in composition [sequence and type of amino acids (AAs)] and structure, which contribute to rapid lysis and have a varied spectrum of antimicrobial activity. AMPs are: peptides (Pps) with alpha-helix structure (i.e., cecropins, magainins, mellitin, etc.); cyclic Pps and ring with several cysteine residues (i.e., defensins, protegrins, etc.); Pps rich in one or another AA (i.e., proline arginine-rich PR39, histidine, glycine, etc.). Most AMPs are characterized by hydrophobic and cationic properties, adopt an amphipathic structure (alpha-helix, spiral beta or alpha-helix / spiral beta), which is essential for antimicrobial activity. AMPs have the potential for therapeutic use in medicine. One of the major concerns of life sciences research is finding new ways to enhance the body’s defense against pathogens. One way is to produce drugs based on AMPs. The AMPs are a class of small Pps that have the ability to destroy pathogens of microbial and viral origin. The mechanisms of action are known only partially and for a small number of AMPs, and the toxic action of AMPs is generally considered to be based on the induction by these molecules in the outer membrane of the pathogen of aqueous pores that facilitate nonspecific ion transfer, which ultimately leads to lysis of the target cell.

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Synergistic activity of chlorhexidine and synoeca‐MP peptide against Pseudomonas aeruginosa

Cited by 8 publications

References 70 publications

Synoeca‐MP: New insights into its mechanism of action by using NMR and molecular dynamics simulations approach

Synoeca‐MP: New insights into its mechanism of action by using NMR and molecular dynamics simulations approach

Insects as sources of food and bioproducts: a review from Colombia

Properties / Characteristics of Antimicrobial Peptides

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