Synergistic action of curcumin and cisplatin on spinel ferrite/hierarchical MCM-41 nanocomposite against MCF-7, HeLa and HCT 116 cancer cell line

Abstract

Background Platinum-based drugs are widely used in cancer therapy, but are known for toxic side effects and resistance. Combinational drug delivery represents an effective chemotherapeutic strategy, but often leads to an increased toxicity. Aim of this study is to test the co-delivery of cisplatin with natural antioxidants on hierarchial porous large surface area hexagonal nanocarriers for synergistic action. Results A series of structured mesoporous materials were impregnated with magnetic spinel ferrite (30% CuFe2O4) and then coated with curcumin (25% wt/wt). Mesosilicalite and MCM-41 with high curcumin release abilities were functionalized with cisplatin (5% wt/wt) for synergistic effect of combinational drugs. The cytotoxic efficiency of our nanocomposites was tested on cell viability of MCF7 (human breast cancer), human cervical cancer (HeLa), colorectal cancer (HCT116), and HFF (human foreskin fibroblasts) cell lines using the MTT cell viability assay. At a concentration of 0.1 mg/ml, CuFe2O4/mesosilicalite/curcumin/cisplatin resulted in 89.53% reduction in viability in MCF7, 94.03% in HeLa, 64% in HCT116 and 87% in HFF; whereas, CuFe2O4/MCM-41/curcumin/cisplatin resulted in 76% reduction in viability in MCF7, 64.46% in HeLa, 64% in HCT116 and 24% in HFF. The EC50 for CuFe2O4/mesosilicalite/curcumin/cisplatin was 81.23 µg/ml in MCF7, 47.55 µg/ml in HeLa, 48.96 µg/ml in HCT116 and 76.83 µg/ml in HFF. The EC50 for CuFe2O4/MCM-41/curcumin/cisplatin was 72.51 µg/ml in MCF7, 58.6 µg/ml in HeLa, 62.58 µg/ml in HCT116 and 154.2 µg/ml in HFF. Furthermore, cells treated with both nanocomposites had a high number of cleaved Caspase 3-positive cells suggesting that the reduction in cell viability was triggered by activating the apoptotic signaling pathway. Conclusion Our results show that CuFe2O4/MCM-41/curcumin/cisplatin is a better candidate for combinational drug therapy due to its lowest EC50 value and the wider difference in EC50 (a fold change) between cancerous and non-cancerous cell line.

Fabrication and biological investigation of a novel star polymer based on magnetic cyclic aromatic polyimide chains

“…Furthermore, at a lower concentration of 5 mg/mL, the cellular viability percentage of healthy HEK293T cells is close to 90%. Meanwhile, in inquiry on CuFe 2 O 4 /mesosilicalite synthesized on HFF (human foreskin fibroblasts) cell lines 46 , where nanocomposite reached only 87% viability at a concentration of only 0.1 mg/mL. In addition, it has been reported that the antibacterial properties of CuFe 2 O 4 nanoparticles have been decreased after being covered or functionalized 47 .…”

Two-dimensional-Ti3C2 magnetic nanocomposite for targeted cancer chemotherapy

“…As part of the cisplatin adsorption study, the following nanocarriers (Ti 3 C 2 -Fe 3 O 4 @SiO 2 -FA and Ti 3 C 2 @ FA) (1 mL) loaded formulations were mixed separately with various masses of Cisplatin (0.5, 1, 1.5, 2, 3 mg) of Cisplatin in 20 mL of Phosphate-buffered saline solution (PBS) under an ice-cooled dark environment ( Jermy et al, 2019 ; 2021 ). After overnight stirring and sonication at room temperature, the solution mixture was centrifuged at 8,000 rpm for 10 min.…”

Fabrication and application of cisplatin-loaded mesoporous magnetic nanobiocomposite: a novel approach to smart cervical cancer chemotherapy

“…Phase separation and ionic gelation methods were mainly implemented to prepare nano-chitosan biocomposites (Ramezani Farani et al 2022;Vaezifar et al 2013). Its functionalization with folic acid is considered a good substance for the delivery of molecules due to its high solubility in water, excellent biocompatibility, and biodegradability (Jermy et al 2021(Jermy et al , 2019. Upon further investigation, it was found that nano-chitosan functionalized by FA behaved highly dependently on the pH value of the solution (Farahnak Roudsari et al 2021).…”