Synergism of Leu–Lys rich antimicrobial peptides and chloramphenicol against bacterial cells

Park, Yoonkyung; Park, Soon Nang; Park, Seong-Cheol; Shin, Sung‐Heui; Kim, Jin‐Young; Kang, Sung-Jin; Kim, Mi‐Hyun; Jeong, Chan-Young; Hahm, Kyung‐Soo

doi:10.1016/j.bbapap.2005.10.019

Cited by 37 publications

(31 citation statements)

References 28 publications

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“…Polycationic a-helical peptides have been reported to exhibit synergistic effects by enhancing the uptake of some antibiotics. [9][10][11][12][13][14][15] Small cationic cyclic peptides from the polymycin family have also been shown to exhibit synergy with small hydrophobic antibiotics. [16] Therefore, these cationic cyclooctapeptides, in their fast acting capacity to compromise the integrity of bacterial membranes, could present themselves as valuable synergists by enhancing the uptake of some antibiotics.…”

Section: Time Course Assaymentioning

confidence: 97%

“…[12] P5-18mer, a Leu-Lys rich, a-helical cationic peptide, was reported to exert a potent synergistic effect with chloramphenicol against various types of bacteria. [15] Additionally, cationic heptapeptides based on polymyxin B, containing 2,4-diaminobutyric acid residues, exert synergistic antibacterial effects by increasing the outer-membrane permeability of novobiocin and erythromycin. [16] Comparable to their linear congeners, some cationic cyclopeptides are efficient bacterial membrane disruptors.…”

Section: Introductionmentioning

confidence: 99%

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In Vitro Synergy Between Some Cationic Amphipathic Cyclooctapeptides and Antibiotics

et al. 2015

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The antimicrobial activities of some cationic amphipathic cyclooctapeptides in combination with select antibiotics are investigated. Accordingly, cyclooctapeptides (CPs 1-11) derived from the sequence cyclo[Leu-D-Leu-Leu-D-Leu-Lys-DLys-Lys-D-Lys] were tested against Escherichia coli and Staphylococcus aureus. Synergistic effects were evaluated in combination with tetracycline, chloramphenicol, oflaxacin, rifampicin, colistin, clindamycin, and vancomycin by the checkerboard titration assay. The results show that these cyclooctapeptides are fast acting bactericidals and are comparable to the related a-helical peptides in their activities. Significantly, some of these cyclooctapeptides exert selective synergistic effects in combination with the bacteriostatic tetracycline against S. aureus; a greater than 4-fold decrease in the minimum inhibition concentration was observed. Their synergism with the other evaluated antibiotics was generally partial or additive. Cationic amphipathic cyclooctapeptides in combination with some antibiotics could offer a possible solution to the increasing antimicrobial resistance predicament.

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Section: Time Course Assaymentioning

confidence: 97%

Section: Introductionmentioning

confidence: 99%

In Vitro Synergy Between Some Cationic Amphipathic Cyclooctapeptides and Antibiotics

et al. 2015

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“…AMPs present a rapid killing and broad-spectrum antimicrobial activities [27]. AMPs are usually composed of 12 to 50 amino acid residues, and they show at least two or more positively charged residues, generally represented by arginine, lysine, or histidine, in acid environments, and with a high content of hydrophobic residues (usually >50%) [28]. Moreover, most AMPs display hydrophobic and cationic properties, have a molecular mass below 25–30 kDa, and adopt an amphipathic structure (alpha-helix, beta-hairpin-like beta-sheet, beta-sheet, or alpha-helix/beta-sheet mixed structures) [29].…”

Section: Crotamine Antimicrobial and Antifungal Activitymentioning

confidence: 99%

State of the Art in the Studies on Crotamine, a Cell Penetrating Peptide from South American Rattlesnake

Kerkis

Hayashi

Silva

et al. 2014

BioMed Research International

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Animal venoms comprise a naturally selected cocktail of bioactive peptides/proteins and other molecules, each of which playing a defined role thanks to the highly specific interactions with diverse molecular targets found in the prey. Research focused on isolation, structural, and functional characterizations of novel natural biologics (bioactive peptides/proteins from natural sources) has a long way to go through from the basic science to clinical applications. Herein, we overview the structural and functional characteristics of the myoneurotoxin crotamine, firstly isolated from the South American rattlesnake venom. Crotamine is the first venom peptide classified as a natural cell penetrating and antimicrobial peptide (CPP and AMP) with a more pronounced antifungal activity. In contrast to other known natural CPPs and AMPs, crotamine demonstrates a wide spectrum of biological activities with potential biotechnological and therapeutic values. More recent studies have demonstrated the selective in vitro anticancer activity of crotamine. In vivo, using a murine melanoma model, it was shown that crotamine delays tumor implantation, inhibits tumor cells proliferation, and also increases the survival of mice engrafted with subcutaneous melanoma. The structural and functional properties and also the possible biotechnological applications of minimized molecules derived from crotamine are also discussed.

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“…For example, an alpha-helical cationic peptide was reported to exert a potent synergistic effect with chloramphenicol against some types of bacteria. 21 …”

mentioning

confidence: 99%

Carbon nanodots as molecular scaffolds for development of antimicrobial agents

Ngu-Schwemlein

Chin

Hileman

et al. 2016

Bioorganic & Medicinal Chemistry Letters

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We report the potential of carbon nanodots (CNDs) as a molecular scaffold for enhancing the antimicrobial activities of small dendritic poly(amidoamines) (PAMAM). Carbon nanodots prepared from sago starch are readily functionalized with PAMAM by using N-ethyl-N′-(3-dimethylaminopropyl)carbodiimide hydrochloride (EDC) and N-hydroxysuccinimide (NHS). Electron microscopy images of these polyaminated CNDs show that they are approximately 30–60 nm in diameter. Infrared and fluorescence spectroscopy analyses of the water-soluble material established the presence of the polyamidoaminated moiety and the intrinsic fluorescence of the nanodots. The polyaminated nanodots (CND-PAM1 and CND-PAM2) exhibit in vitro antimicrobial properties, not only to nonmultidrug resistant bacteria but also to the corresponding Gram-negative multidrug bacteria. Their minimum inhibitory concentration (MIC) ranges from 8 to 64 µg/mL, which is much lower than that of PAMAM G1 or the non-active PAMAM G0 and CNDs. Additionally, they show synergistic effect in combination with tetracycline or colistin. These preliminary results imply that CNDs can serve as a promising scaffold for facilitating the rational design of antimicrobial materials for combating the ever-increasing threat of antibiotic resistance. Moreover, their fluorescence could be pertinent to unraveling their mode of action for imaging or diagnostic applications.

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Synergism of Leu–Lys rich antimicrobial peptides and chloramphenicol against bacterial cells

Cited by 37 publications

References 28 publications

In Vitro Synergy Between Some Cationic Amphipathic Cyclooctapeptides and Antibiotics

In Vitro Synergy Between Some Cationic Amphipathic Cyclooctapeptides and Antibiotics

State of the Art in the Studies on Crotamine, a Cell Penetrating Peptide from South American Rattlesnake

Carbon nanodots as molecular scaffolds for development of antimicrobial agents

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