Synergism between Bacillus thuringiensis and Xenorhabdus nematophila against resistant and susceptible Plutella xylostella (Lepidoptera: Plutellidae)

Nangong, Ziyan; Wang, Qinying; Song, Ping; Hao, Jie; Yang, Qing; Wang, Li‐Yu

doi:10.1080/09583157.2016.1212186

Cited by 4 publications

(5 citation statements)

References 40 publications

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“…By concurrently administering the combination of Bt and Xn, we observed that the mortality of SWD larvae increased by approximately 10%; the most interesting effect was the reduction in the time required to obtain a significant lethality, a mortality rate of 67% was already recorded at 16 h. The combination of Bt with Xn secretions resulted in a marked increase in mortality, which reached 86% at 24 h, and 100% at 48 h. Overall, the observed increases in efficacy using combinations of bioinsecticides agree with the literature [ 52 , 55 , 56 ]. In particular, our results are similar to those obtained with the combination of Bt and Xn when used on the dipteran Aedes albopictus, Culex pipiens pallens and on the lepidopteran P. xylostella [ 21 , 50 , 57 ].…”

Section: Discussionsupporting

confidence: 85%

Drosophila suzukii Susceptibility to the Oral Administration of Bacillus thuringiensis, Xenorhabdus nematophila and Its Secondary Metabolites

Mastore

Caramella

Quadroni

et al. 2021

Insects

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Drosophila suzukii, Spotted Wing Drosophila (SWD), is a serious economic issue for thin-skinned fruit farmers. The invasion of this dipteran is mainly counteracted by chemical control methods; however, it would be desirable to replace them with biological control. All assays were performed with Bacillus thuringiensis (Bt), Xenorhabdus nematophila (Xn), and Xn secretions, administered orally in single or combination, then larval lethality was assessed at different times. Gut damage caused by Bt and the influence on Xn into the hemocoelic cavity was also evaluated. In addition, the hemolymph cell population was analyzed after treatments. The data obtained show that the combined use of Bt plus Xn secretions on larvae, compared to single administration of bacteria, significantly improved the efficacy and reduced the time of treatments. The results confirm the destructive action of Bt on the gut of SWD larvae, and that Bt-induced alteration promotes the passage of Xn to the hemocoel cavity. Furthermore, hemocytes decrease after bioinsecticides treatments. Our study demonstrates that combining bioinsecticides can improve the efficacy of biocontrol and such combinations should be tested in greenhouse and in field in the near future.

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Section: Discussionsupporting

confidence: 85%

Drosophila suzukii Susceptibility to the Oral Administration of Bacillus thuringiensis, Xenorhabdus nematophila and Its Secondary Metabolites

Mastore

Caramella

Quadroni

et al. 2021

Insects

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“…La resistencia desarrollada por la P. xylostella ha llevado a la investigación a descubrir nuevas proteínas insecticidas activas oralmente, así como a evaluar los efectos combinados de Bt y otros organismos con el fin de retrasar y combatir la propagación de este insecto plaga (Nangong, Wang, Song, Hao, Yang y Wang, 2016). Nangong, et al (2016) evaluaron mediante cl 50 el daño combinado y separado de las bacterias Xenorhabdus nematophila (Xn hb310), Bt hd-1 y Bt hd-73 sobre poblaciones de la P. xylostella.…”

Section: Mecanismo De Acción De Las Toxinas Cryunclassified

“…Nangong, et al (2016) evaluaron mediante cl 50 el daño combinado y separado de las bacterias Xenorhabdus nematophila (Xn hb310), Bt hd-1 y Bt hd-73 sobre poblaciones de la P. xylostella. Para ello, obtuvieron de la Universidad Agrícola de Hebei, de China, tres cepas de las bacterias: X. nematophila (Xn hb310), Bt hd-1 (que contiene los genes Cry1Aa, Cry1Ab, Cry1Ac, Cry1Ia, Cry2Aa, Cry2Ab y Vip3Aa) y Bt hd-73 (que contiene el gen Cry1Ac).…”

Section: Mecanismo De Acción De Las Toxinas Cryunclassified

Brasicáceas y perspectivas de control biológico del insecto plaga Plutella xylostella (Lepidóptera: Plutellidae) utilizando Bacillus thuringiensis

Guerrero

Fernández

2017

Revista Mutis

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La Plutella xylostella (L.) es un insecto plaga que afecta principalmente los cultivos de brasicáceas o crucíferas como el repollo, coliflor, brócoli y rábano, entre otros. Las limitaciones del rendimiento y calidad de estos cultivos se deben principalmente al difícil manejo de esta plaga. En la actualidad, para controlar la P. xylostella se utilizan insecticidas de síntesis química, como piretroides, carbamatos u organofosforados, los cuales tienen acción inmediata pero causan efectos adversos en la salud y en el ambiente. Adicionalmente, la ausencia de enemigos naturales capaces de controlar a sus poblaciones, y su resistencia a numerosos insecticidas convencionales hacen que este insecto se establezca rápidamente en áreas productoras de brasicáceas. Se calcula que los costos mundiales asociados al control de la P. xylostella, sumados a las pérdidas en la producción agrícola, están entre cuatro y cinco billones de dólares anuales.Se han propuesto alternativas seguras, efectivas y de menor impacto ambiental, como el control biológico, que permite la producción sostenible de los cultivos de brasicáceas. La industria agrícola y forestal reconoce a la bacteria entomopatógena Bacillus thuringiensis (Bt) como una buena alternativa biológica a los insecticidas químicos, puesto que es inocua sobre el ambiente y su toxicidad es altamente selectiva, ligada a su estrecho rango de especificidad sobre diferentes insectos plaga de los órdenes Lepidóptera, Coleóptera y Díptera. En esta revisión se presentan las posibilidades actuales que se pueden emplear para el control de la P. xylostella utilizando modelos investigativos basados en ensayos biológicos con Bt. Dichas posibilidades buscan superar las desventajas existentes en relación con la plaga P. xylostella y los cultivos de brasicáceas, además de entender la fisiología de Bt bajo condiciones que permitan incrementar la eficacia en el control biológico.

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“…In addition, some species of Xenorhabdus have been reported to synergize the insecticidal activity of B. thuringiensis against some insect pests such as mosquitoes [ 19 , 20 , 21 ], turning them into potential supplementary ingredients for improving B. thuringiensis -based insecticides.…”

Section: Introductionmentioning

confidence: 99%

Genome Sequence Analysis of Native Xenorhabdus Strains Isolated from Entomopathogenic Nematodes in Argentina

Palma,

Frizzo,

Kaiser

et al. 2024

Toxins

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Entomopathogenic nematodes from the genus Steinernema (Nematoda: Steinernematidae) are capable of causing the rapid killing of insect hosts, facilitated by their association with symbiotic Gram-negative bacteria in the genus Xenorhabdus (Enterobacterales: Morganellaceae), positioning them as interesting candidate tools for the control of insect pests. In spite of this, only a limited number of species from this bacterial genus have been identified from their nematode hosts and their insecticidal properties documented. This study aimed to perform the genome sequence analysis of fourteen Xenorhabdus strains that were isolated from Steinernema nematodes in Argentina. All of the strains were found to be able of killing 7th instar larvae of Galleria mellonella (L.) (Lepidoptera: Pyralidae). Their sequenced genomes harbour 110 putative insecticidal proteins including Tc, Txp, Mcf, Pra/Prb and App homologs, plus other virulence factors such as putative nematocidal proteins, chitinases and secondary metabolite gene clusters for the synthesis of different bioactive compounds. Maximum-likelihood phylogenetic analysis plus average nucleotide identity calculations strongly suggested that three strains should be considered novel species. The species name for strains PSL and Reich (same species according to % ANI) is proposed as Xenorhabdus littoralis sp. nov., whereas strain 12 is proposed as Xenorhabdus santafensis sp. nov. In this work, we present a dual insight into the biocidal potential and diversity of the Xenorhabdus genus, demonstrated by different numbers of putative insecticidal genes and biosynthetic gene clusters, along with a fresh exploration of the species within this genus.

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Synergism between Bacillus thuringiensis and Xenorhabdus nematophila against resistant and susceptible Plutella xylostella (Lepidoptera: Plutellidae)

Cited by 4 publications

References 40 publications

Drosophila suzukii Susceptibility to the Oral Administration of Bacillus thuringiensis, Xenorhabdus nematophila and Its Secondary Metabolites

Drosophila suzukii Susceptibility to the Oral Administration of Bacillus thuringiensis, Xenorhabdus nematophila and Its Secondary Metabolites

Brasicáceas y perspectivas de control biológico del insecto plaga Plutella xylostella (Lepidóptera: Plutellidae) utilizando Bacillus thuringiensis

Genome Sequence Analysis of Native Xenorhabdus Strains Isolated from Entomopathogenic Nematodes in Argentina

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