Synergism and mechanism of Astragaloside IV combined with Ginsenoside Rg1 against autophagic injury of PC12 cells induced by oxygen glucose deprivation/reoxygenation

Huang, Xiaoping; Ding, Huang; Yang, Xiaoqian; Li, Jingxian; Tang, Biao; Liu, Xiaodan; Tang, Yinghong; Deng, Chang-Qing

doi:10.1016/j.biopha.2017.02.015

Cited by 23 publications

(11 citation statements)

References 38 publications

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“…The characteristics of PLA patients in northeast China supported the previously reported findings that PLA mainly occurred in middle‐aged males with a single abscess in the right hemiliver 6,10,11 . The most common clinical presentation was fever (88.9%), followed by abdominal pain (51.3%), which were similar to the findings reported in previous studies 10,12‐15 …”

Section: Discussionsupporting

confidence: 89%

Clinical characteristics and management of 1572 patients with pyogenic liver abscess: A 12‐year retrospective study

Yin

Zhang

et al. 2020

Liver International

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Background & aims Pyogenic liver abscesses (PLA) are space‐occupying lesions in the liver that produce high morbidity and mortality. The clinical characteristics and prognosis of abscesses is different depending on the bacterial culture results and require different strategies for management. The aim of this study was to investigate the clinical characteristics and prognostic factors of patients with PLA. Methods Clinical features, laboratory tests and etiology of PLA between 2006 to 2011 and 2012 to 2017 in a single hospital were retrospectively reviewed. The incidence and mortality of PLA caused by Escherichia coli and Klebsiella pneumoniae were compared and the risk factors for multiple organ dysfunction (MODS) and endophthalmitis were evaluated. Results Among the 1,572 PLA patients, the proportion with PLA increased from 333 (21.2%) in 2006‐2011 to 1,239 (78.8%) in 2012‐2017 without any investigation and treatment procedure differences. K pneumoniae was the main isolate in analysed pus cultures (85.6%). The mortality rate of patients with K pneumoniae infection was lower in the latter period (6.7% vs 0.7%, P = .035). Multivariate analyses revealed that age, fever, MODS and length of hospital stay were factors affecting poor prognosis (death + unhealed/uncured) in PLA patients after treatment and that cardiovascular disease, pleural effusion and pulmonary infection were risk factors for MODS, while diabetes mellitus was the only risk factor for endophthalmitis. Most patients (95.5%) with PLA recovered after abscess drainage/puncture and antibiotic therapy. Conclusions Pleural effusion, fever, MODS and length of hospital stays were factors useful in predicting PLA outcomes.

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Section: Discussionsupporting

confidence: 89%

Clinical characteristics and management of 1572 patients with pyogenic liver abscess: A 12‐year retrospective study

Yin

Zhang

et al. 2020

Liver International

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“…Chang et al found that ganoderic acid A could alleviate the autophagy effect of rat neural stem cells activated by hypoxia injury by activating the PI3K I/AKT/mTOR pathway [44]. Huang et al reported that the combination of Ginsenoside Rg1 and Astragaloside IV inhibited OGD/R-induced autophagy in PC12 cells by regulating PI3K III/Beclin-1/Bcl-2 and PI3K I/ Akt/mTOR pathways [45]. Therefore, the results of this study suggested that germacrone inhibited the activation of OGD/R-induced autophagy in PC12 cells by blocking the PI3K III/Beclin-1/Bcl-2 pathway and activating the PI3K I/Akt/mTOR pathway.…”

Section: Discussionmentioning

confidence: 99%

Germacrone protects against oxygen-glucose deprivation/reperfusion injury by inhibiting autophagy processes in PC12 cells

Zhang

Wang

et al. 2020

BMC Complement Med Ther

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Background: Germacrone is an anti-inflammatory ingredient in the Chinese medicine zedoary turmeric. The purpose of this study was to explore the protective mechanism of germacrone against PC12 cells injury caused by oxygen-glucose deprivation/reperfusion (OGD/R). Methods: OGD/R injury model of PC12 cells was established by using OGD/R (2 h/24 h). The cell viability was assessed by MTT assay and LDH release. The ultrastructure of cells was observed by transmission electron microscopy (TEM). The expression of autophagy related proteins in cells was determined by Western Blot. Results: The results of ultrastructural observation showed that PC12 cells damaged by OGD/R showed typical autophagy characteristics. In addition, OGD/R observably up-regulated the expression of autophagy related proteins: the class III type phosphoinositide 3-kinase (PI3K III), light chain 3(LC3), and Beclin-1 in PC12 cells, and inhibited the expression of the class I type phosphoinositide 3-kinase (PI3K I), Protein kinase B (Akt), the mammalian target of rapamycin (mTOR), and B-cell lymphoma 2(Bcl-2) proteins. Furthermore, germacrone increased the cell viability of OGD/R-damaged PC12 cells by down-regulating the expression of LC3 protein in cells in a concentration-dependent manner. More importantly, germacrone significantly inhibited the expression of PI3K III, LC3, and Beclin-1 in OGD/R-injured PC12 cells, and up-regulated the expressionof PI3K I, Akt, mTOR, and Bcl-2 proteins in cells, and this inhibited or up-regulated effect was reversed by PI3K I inhibitor (ZSTK474). Conclusion: The above results indicated that germacrone could inhibit the autophagy effect in OGD/R injury model of PC12 cells, the mechanism of inhibition was regulated by PI3K III/Beclin-1/Bcl-2 and PI3K I/Akt/mTOR pathways, thereby improving the cell viability of PC12 cells and playing a neuroprotective role, which provided a new drug for the treatment of OGD/R.

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“…This is the first report that this combination potentiates anorectic effects. Diethylpropion + topiramate combination was tested at 1:1, 1:3, and 3:1 dose ratios as the type of interaction between the two drugs depends on the dose ratio (Luszczki & Czuczwar, ; Huang et al, ). In our study, all proportions show an anorectic interaction of potentiation in a manner independent of the experimental paradigm, indicating that both diethylpropion and topiramate contribute to the observed anorectic effect.…”

Section: Discussionmentioning

confidence: 99%

Anorectic efficacy and safety of the diethylpropion‐topiramate combination in rats

Cortés-Moreno

Roa-Coria

Zúñiga-Romero

et al. 2018

Drug Development Research

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Preclinical Research & Development Current drugs for obesity treatment have limited efficacy and considerable adverse effects. Combination of drugs with complementary mechanisms of action at lower doses may produce a greater efficacy with a better safety profile. This study was designed to assess the anorectic effect and safety of a diethylpropion + topiramate mixture in rats. The anorectic effect of drugs was measured using a sweetened milk consumption model, and the corresponding interaction was determined by isobolographic analysis, interaction index and confidence intervals. Additionally, blood pressure was measured using a sphygmomanometer in the rat tail. Diethylpropion and topiramate alone or in combination increased the anorectic effect in a dose-dependent fashion in either nondeprived or 12 hr food-deprived rats. All theoretical ED values of diethylpropion + topiramate combinations at 1:1, 1:3, and 3:1 dose ratios were significantly higher than experimental ED values. In addition, interaction indices and confidence intervals confirmed the potentiation between both drugs. Theoretical ED of diethylpropion + topiramate combination did not affect the blood pressure. Data suggests that low doses of the diethylpropion + topiramate combination can potentiate the anorectic effect of individual drugs with a better safety profile, which deserves further investigation in clinical trials.

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Synergism and mechanism of Astragaloside IV combined with Ginsenoside Rg1 against autophagic injury of PC12 cells induced by oxygen glucose deprivation/reoxygenation

Cited by 23 publications

References 38 publications

Clinical characteristics and management of 1572 patients with pyogenic liver abscess: A 12‐year retrospective study

Clinical characteristics and management of 1572 patients with pyogenic liver abscess: A 12‐year retrospective study

Germacrone protects against oxygen-glucose deprivation/reperfusion injury by inhibiting autophagy processes in PC12 cells

Anorectic efficacy and safety of the diethylpropion‐topiramate combination in rats

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