Synergic Effects of Temperature and Irradiance on the Physiology of the Marine Synechococcus Strain WH7803

Guyet, Ulysse; Nguyen, Ngoc-An; Doré, Hugo; Haguait, Julie; Pittera, Justine; Conan, Maël; Ratin, Morgane; Corre, Erwan; Corguillé, Gildas Le; Brillet-Guéguen, Loraine; Hoebeke, Mark; Six, Christophe; Steglich, Claudia; Siegel, Anne; Eveillard, Damien; Partensky, Frédéric; Garczarek, Laurence

doi:10.3389/fmicb.2020.01707

Cited by 19 publications

(22 citation statements)

References 145 publications

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“…In the current version of Cyanorak, transcriptomic data are available for Synechococcus sp. WH7803 ( 29 , 34 ) and a number of other Prochlorococcus or Synechococcus strains studied by other groups, but in this case expression data are only displayed as log2(Fold Change).…”

Section: Resultsmentioning

confidence: 99%

“…In Cyanorak, these data are connected to the genome database through a JBrowse interface, which also gives access to genomic features such as predicted operons, gained genes or the core or accessory nature of genes, which can be used to further refine the interpretation of gene expression data (see e.g. ( 34 )).…”

Section: Discussionmentioning

confidence: 99%

“…Two instances of the Cyanorak information system therefore co-exist on our server: (i) the restricted access, editable Cyanorak v2.0 version allowing expert curators to edit most fields of the ‘CLOG’ and ‘gene’ pages and (ii) the publicly accessible, non-editable Cyanorak v2.1 version, the latter corresponding to the state of the Cyanorak database at the time of publication of a comparative genomics study of the 81 non-redundant, high quality genomes of the database ( 9 ) and of an extensive transcriptomic analysis of the response of the Synechococcus sp. WH7803 strain to various environmental stresses ( 34 ). This public version will be regularly updated in the future, with concomitant changes in version number, when new whole genome sequences (WGS), single amplified- and metagenome assembled- genomes (SAGs, MAGs) and/or transcriptomes either retrieved from public databases (e.g.…”

Section: Methodsmentioning

confidence: 99%

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Cyanorak v2.1: a scalable information system dedicated to the visualization and expert curation of marine and brackish picocyanobacteria genomes

Garczarek

Guyet

Doré

et al. 2020

Nucleic Acids Research

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Cyanorak v2.1 (http://www.sb-roscoff.fr/cyanorak) is an information system dedicated to visualizing, comparing and curating the genomes of Prochlorococcus, Synechococcus and Cyanobium, the most abundant photosynthetic microorganisms on Earth. The database encompasses sequences from 97 genomes, covering most of the wide genetic diversity known so far within these groups, and which were split into 25,834 clusters of likely orthologous groups (CLOGs). The user interface gives access to genomic characteristics, accession numbers as well as an interactive map showing strain isolation sites. The main entry to the database is through search for a term (gene name, product, etc.), resulting in a list of CLOGs and individual genes. Each CLOG benefits from a rich functional annotation including EggNOG, EC/K numbers, GO terms, TIGR Roles, custom-designed Cyanorak Roles as well as several protein motif predictions. Cyanorak also displays a phyletic profile, indicating the genotype and pigment type for each CLOG, and a genome viewer (Jbrowse) to visualize additional data on each genome such as predicted operons, genomic islands or transcriptomic data, when available. This information system also includes a BLAST search tool, comparative genomic context as well as various data export options. Altogether, Cyanorak v2.1 constitutes an invaluable, scalable tool for comparative genomics of ecologically relevant marine microorganisms.

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Section: Resultsmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

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Cyanorak v2.1: a scalable information system dedicated to the visualization and expert curation of marine and brackish picocyanobacteria genomes

Garczarek

Guyet

Doré

et al. 2020

Nucleic Acids Research

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“…Clustering of genes according to their temporal waveforms suggested that master transcriptional regulations are at work to coordinate lipid metabolism with chloroplast and carotenoids biogenesis at late night and with photosynthesis, oxidative stress, and DNA repair at mid-day. Transcriptional rewiring in response to temperature, especially chilling, has been extensively studied in freshwater cyanobacteria and plants and are beginning to be studied in marine cyanobacteria ( Sinetova and Los, 2016 ; Shi et al, 2018 ; Breton et al, 2020 ; Guyet et al, 2020 ). However, there is a large gap of knowledge with regards to microalgae.…”

Section: Discussionmentioning

confidence: 99%

Temperature Acclimation of the Picoalga Ostreococcus tauri Triggers Early Fatty-Acid Variations and Involves a Plastidial ω3-Desaturase

Degraeve-Guilbault

Pankasem²,

Gueirrero³

et al. 2021

Front. Plant Sci.

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Alteration of fatty-acid unsaturation is a universal response to temperature changes. Marine microalgae display the largest diversity of polyunsaturated fatty-acid (PUFA) whose content notably varies according to temperature. The physiological relevance and the molecular mechanisms underlying these changes are however, still poorly understood. The ancestral green picoalga Ostreococcus tauri displays original lipidic features that combines PUFAs from two distinctive microalgal lineages (Chlorophyceae, Chromista kingdom). In this study, optimized conditions were implemented to unveil early fatty-acid and desaturase transcriptional variations upon chilling and warming. We further functionally characterized the O. tauri ω3-desaturase which is closely related to ω3-desaturases from Chromista species. Our results show that the overall omega-3 to omega-6 ratio is swiftly and reversibly regulated by temperature variations. The proportion of the peculiar 18:5 fatty-acid and temperature are highly and inversely correlated pinpointing the importance of 18:5 temperature-dependent variations across kingdoms. Chilling rapidly and sustainably up-regulated most desaturase genes. Desaturases involved in the regulation of the C18-PUFA pool as well as the Δ5-desaturase appear to be major transcriptional targets. The only ω3-desaturase candidate, related to ω3-desaturases from Chromista species, is localized at chloroplasts in Nicotiana benthamiana and efficiently performs ω3-desaturation of C18-PUFAs in Synechocystis sp. PCC6803. Overexpression in the native host further unveils a broad impact on plastidial and non-plastidial glycerolipids illustrated by the alteration of omega-3/omega-6 ratio in C16-PUFA and VLC-PUFA pools. Global glycerolipid features of the overexpressor recall those of chilling acclimated cells.

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“…In order to test this hypothesis, we used strains representative of each of the three CRD1 ESTUs to determine the fundamental thermal niches of these organisms as compared to typical cold (clades I and IV) and warm (clades II and III) thermotypes. Furthermore, given the strong influence of temperature on optimal functioning of the photosynthetic apparatus in marine Synechococcus (Pittera et al, 2014(Pittera et al, , 2017Guyet et al, 2020), we also examined the effect of temperature acclimation on the photophysiology of CRD1 ESTUs compared to their clade I and IV counterparts and show that CRD1 thermotypes actually differ more strongly in this respect to members of clades I-IV than from each other.…”

Section: Introductionmentioning

confidence: 99%

Comparative thermophysiology of marine Synechococcus CRD1 strains isolated from different thermal niches in iron-depleted areas

Ferrieux

Dufour

Doré

et al. 2022

Preprint

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Marine Synechococcus cyanobacteria are ubiquitous in the ocean, a feature likely related to their extensive genetic diversity. Amongst the major lineages, clades I and IV preferentially thrive in temperate and cold, nutrient-rich waters, whilst clades II and III prefer warm, nitrogen or phosphorus-depleted waters. The existence of such cold (I/IV) and warm (II/III) thermotypes is corroborated by physiological characterization of representative strains. A fifth clade, CRD1, was recently shown to dominate the Synechococcus community in iron-depleted areas of the world ocean and to encompass three distinct ecologically significant taxonomic units (ESTUs CRD1A-C) occupying different thermal niches, suggesting that distinct thermotypes could also occur within this clade.Here, using comparative thermophysiology of strains representative of these three CRD1 ESTUs we show that the CRD1A strain MITS9220 is a warm thermotype, the CRD1B strain BIOS-U3-1 a cold temperate thermotype, and the CRD1C strain BIOS-E4-1 a warm temperate stenotherm. Curiously, the CRD1B thermotype lacks traits and/or genomic features typical of cold thermotypes. In contrast, we found specific physiological traits of the CRD1 strains compared to their clade I, II, III and IV counterparts, including a lower growth rate and photosystem II maximal quantum yield at most temperatures and a higher turnover rate of the D1 protein. Together, our data suggests that the CRD1 clade prioritizes adaptation to low-iron conditions over temperature adaptation, even though the occurrence of several CRD1 thermotypes likely explains why the CRD1 clade as a whole occupies most iron-limited waters.

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Synergic Effects of Temperature and Irradiance on the Physiology of the Marine Synechococcus Strain WH7803

Cited by 19 publications

References 145 publications

Cyanorak v2.1: a scalable information system dedicated to the visualization and expert curation of marine and brackish picocyanobacteria genomes

Cyanorak v2.1: a scalable information system dedicated to the visualization and expert curation of marine and brackish picocyanobacteria genomes

Temperature Acclimation of the Picoalga Ostreococcus tauri Triggers Early Fatty-Acid Variations and Involves a Plastidial ω3-Desaturase

Comparative thermophysiology of marine Synechococcus CRD1 strains isolated from different thermal niches in iron-depleted areas

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