Synergetic role of senna and fennel extracts as antioxidant, anti-inflammatory and anti-mutagenic agents in irradiated human blood lymphocyte cultures

Farid, Alyaa; Kamel, Dina; Montaser, Sherien Abdelwahab; Ahmed, M. M.; Amir, Mona El; Amir, Azza El

doi:10.1080/16878507.2020.1723948

Cited by 19 publications

(9 citation statements)

References 44 publications

(44 reference statements)

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“…Bogucka-Kocka, Smolarz, and Kocki (2008) reported the apoptotic activities of ethanolic extracts of fennel on human leukemia cell lines. Also, Farid et al (2020) reported that the combination between fennel as an apoptic inducer and calcium sennosides as a free radicals scavenger can be used for post-radiation treatment.…”

Section: Discussionmentioning

confidence: 99%

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Assessment of antioxidant, immune enhancement, and antimutagenic efficacy of fennel seed extracts in irradiated human blood cultures

Farid

Kamel

Montaser

et al. 2020

Journal of Radiation Research and Applied Sciences

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Foeniculum vulgare Mill. (commonly known as fennel) is one of the precious spices. Almost all parts of the plant are edible and have powerful interest in phytochemical and pharmacological research. The present study was designed to examine the free radical scavenging potential, oxidative damage preventive activity, and anti-inflammatory effects of traditionally used fennel. It assessed the cytogenetic and biochemical effects of fennel on suppression of the radiation hazards in human blood culture at a working dose of 200µg/ml. The treatment time was 72 h post-irradiation (3 Gy γ-irradiation). Triple blood cultures for each blood sample were set up. Ionizing radiation exposure induced significant increase in micronuclei (MN) frequencies in both mono-and binucleated cells beside significant decrease in superoxide dismutase (SOD) and catalase (CAT) activities and increase in lactate dehydrogenase (LDH). While Interleukin (IL)-1β, −6, −8 and Tumor Necrosis Factor (TNF)-α levels were significantly increased after irradiation. Treatment with fennel exhibited a decrease in micronuclei and enhancement in the level of SOD, CAT, and LDH activities. In addition, significant amelioration in IL-1β, 6 and 8, TNF-α activity was scored. The present results revealed the antimutagenic, anti-inflammatory and antioxidant effects of fennel against oxidative stress induced by γirradiation.

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Section: Discussionmentioning

confidence: 99%

“…They added that 200 μg/ml of fennel alcoholic extract showed an anti-tumor activity against B16F10 melanoma cell line. Also, Farid et al (2020) reported the use of fennel as an apoptic inducer for post-radiation treatment against oxidative and inflammation effects of ionizing radiation.…”

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confidence: 99%

Assessment of antioxidant, immune enhancement, and antimutagenic efficacy of fennel seed extracts in irradiated human blood cultures

Farid

Kamel

Montaser

et al. 2020

Journal of Radiation Research and Applied Sciences

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“…MBS700021). All measured immunological parameters were evaluated by sandwich ELISA due to its effectivety and high efficacy in detection according to ( Farid et al, 2019 ; Farid et al, 2020b ; Farid et al, 2020c ; Farid et al, 2020d ). The concentrations of serum interleukin (IL)-6, -8 and -10 were measured by using mouse ELISA kit (ab100712, MBS261967 and ab100697, respectively).…”

Section: Methodsmentioning

confidence: 99%

The hidden hazardous effects of stevia and sucralose consumption in male and female albino mice in comparison to sucrose

Farid

Hesham

El-Dewak

et al. 2020

Saudi Pharmaceutical Journal

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Replacing sucrose with non-caloric sweeteners is an approach to avoid overweight and diabetes development. Non-caloric sweeteners are classified into either artificial as sucralose or natural as stevia. Both of them have been approved by FDA, but the effects of their chronic consumption are controversial. The present study aimed to evaluate the effects of these two sweeteners, in male and female albino mice, on different blood biochemical parameters, enzymes activities and immunological parameters after 8 and 16 weeks of sweeteners administration. 40.5 mg/ml of sucrose, 5.2 mg/ml of sucralose and 4.2 mg/ml of stevia were dissolved individually in distilled water. Mice were administrated by sweetener's solution for 5 h daily. Male and female mice showed a preference for water consumption with sucralose or stevia. Both of the two sweeteners significantly reduced the hemoglobin level, HCT%, RBCs and WBCs count. After 18 weeks, significant elevations in liver and kidney function enzymes were observed in male and female mice administrated with both non-caloric sweeteners. Histopathological examination in sucralose and stevia administrated groups confirmed the biochemical results; where it revealed a severe damage in liver and kidney sections. While, sucrose administration elevated, only, the levels of ALT, AST and cholesterol in male mice. A vigorous elevation in levels of different immunoglobulin (IgG, IgE and IgA) and pro-inflammatory cytokines (IL-6 and -8), that was accompanied by a significant reduction in level of anti-inflammatory cytokine IL-10, was observed in male and female mice groups administrated with sucralose or stevia. On the other hand, sucrose administration led to an elevation in IgA and reduction in IL-10 levels.

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“…Kryston et al (51) reported that irradiation led to the induction of ROS and RNS that disrupt the normal redox balance inside the cells leading to severe inflammatory processes. Farid et al (52)(53) reported that many pro-inflammatory cytokines were released shortly after irradiation such as IL -1β, IL-6 and which were of particular importance in radiation toxicity pathophysiology. Farid et al (54) added that cytokines are low molecular weight proteins that have a stimulating or inhibiting effect on immune system cells' proliferation, differentiation and function.…”

Section: Int J Radiat Res Vol 19 No 4 October 2021mentioning

confidence: 99%

Anti-apoptotic and antioxidant effects of melatonin protect spleen of whole body γ-irradiated male Sprague-dawley rats

2021

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Background: Spleen is the largest lymphatic organ that is seriously affected during irradiation. Radiation exposure reduces both of spleen size and weight; that in turn decreases the numbers of immune cells. Melatonin is an effective free radicals scavenger. Therefore, this study aimed to evaluate the effects of melatonin on both blood and spleen of whole body γ-irradiated male Sprague dawley rats. Materials and methods: Animals were intraperitoneally injected with 100 mg/kg melatonin prior to radiation exposure by 30 minutes. Experimental groups were group I: control rats, group II: irradiated rats, group III: melatonin administrated unirradiated rats and group IV: melatonin administrated irradiated rats. Blood and spleen samples were collected 24 hours post irradiation for biochemical, immunological and blastogenesis measurements. Apoptosis, pro-and anti-apoptotic proteins of spleen cells were measured by flow cytometry techniques. Results: Melatonin significantly upregulated the activities of superoxide dismutase (SOD), glutathione (GSH) and catalase (CAT); and reduced malondialdehyde (MDA). It down regulated the expression of pro-apoptotic proteins (p53, Bax, caspase -3 and caspase-8) and up regulated the expression of anti-apoptotic protein Bcl-2 in spleen cells; that in turn reduced the radiation-induced apoptosis. Levels of pro-inflammatory cytokines (TNF-α, IFN-γ and IL-1β) were significantly reduced in group IV. Blastogenesis assay showed that melatonin protects PBMC and spleen B lymphocytes and stabilized their proliferation. Conclusion: Melatonin administration prior to whole body γ-radiation successfully protected rat's spleen from the consequences of radiation exposure. This was due to its free radicle scavenger nature, its reduction of lipid peroxidation and its anti-apoptotic effects.

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Synergetic role of senna and fennel extracts as antioxidant, anti-inflammatory and anti-mutagenic agents in irradiated human blood lymphocyte cultures

Cited by 19 publications

References 44 publications

Assessment of antioxidant, immune enhancement, and antimutagenic efficacy of fennel seed extracts in irradiated human blood cultures

Assessment of antioxidant, immune enhancement, and antimutagenic efficacy of fennel seed extracts in irradiated human blood cultures

The hidden hazardous effects of stevia and sucralose consumption in male and female albino mice in comparison to sucrose

Anti-apoptotic and antioxidant effects of melatonin protect spleen of whole body γ-irradiated male Sprague-dawley rats

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