Synergetic promotion of direct interspecies electron transfer for syntrophic metabolism of propionate and butyrate with graphite felt in anaerobic digestion

Zhang, Mingyuan; Ma, Yunqian; Li, Xueyuan; Zhang, Jishi; Ji, Dandan

doi:10.1016/j.biortech.2019.121373

Cited by 74 publications

(24 citation statements)

References 36 publications

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“…[Figure 1 here] The observed decrease in acetic, propionic, and butyric acid concentrations is in accordance with existing literature. A recent study at laboratory scale demonstrated that conductive materials help lower the concentrations of propionic and butyric acids, and explained that this phenomenon is due to an increased rate of DIET, which in turn reduced the amount of inhibiting hydrogen [7]. The here presented results confirm the effect of conductive materials on organic acid concentrations and demonstrate this phenomenon for the first time at an industrial scale.…”

Section: Influence Of Biochar On the Spectrum Of Organic Acidssupporting

confidence: 85%

“…However, the first mechanisms have already been proposed. According to an article by Zhang et al (2019), DIET contributed to lower hydrogen partial pressures, which in turn lowered the concentration of butyric acid [7].…”

Section: Introductionmentioning

confidence: 99%

“…If hydrogen consumption is inhibited, or if hydrogen production is too fast, a slight increase in the hydrogen pressure might take place and inhibit the syntrophic degradation [8]. As conductive particles allow direct electron transport without the need for hydrogen interspecies transfer (HIT), this explains the enhancement in syntrophic butyric acid degradation described by Zhang et al (2019), as previously mentioned.…”

Section: Introductionmentioning

confidence: 99%

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Monitoring of seven industrial anaerobic digesters supplied with biochar

Heitkamp

Latorre‐Pérez²,

Nefigmann³

et al. 2021

Preprint

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Background: Recent research articles indicate that direct interspecies electron transfer (DIET) is an alternative metabolic route for methanogenic archaea that improves microbial methane productivity. It has been shown that multiple conductive materials such as biochar can be supplemented to anaerobic digesters to increase the rate of DIET. However, the industrial applicability, as well as the impact of such supplements on taxonomic profiles, has not been sufficiently assessed to date. Results: Seven industrial anaerobic digesters were supplemented with biochar for one year. A positive effect was observed for the spectrum of organic acids as the concentration of acetic, propionic, and butyric acid decreased significantly. Quantification of the cofactor F420 using fluorescence microscopy showed a reduction in methanogenic archaea. 16S-rRNA gene amplicon sequencing showed a higher microbial diversity within biochar particles as well as an accumulation of secondary fermenters and halotolerant bacteria. Taxonomic profiles indicate microbial electroactivity, and show the frequent occurrence of Methanoculleus , which has not been described in this context before. Conclusions: Our results shed light on the interplay between biochar particles and microbial communities in anaerobic digesters. Both the microbial diversity and the absolute frequency of the microorganisms involved were significantly changed between sludge samples and biochar particles. This is particularly important against the background of microbial process monitoring. In addition, it could be shown that biochar is suitable for reducing the content of inhibitory, volatile acids on an industrial scale.

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Section: Influence Of Biochar On the Spectrum Of Organic Acidssupporting

confidence: 85%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Monitoring of seven industrial anaerobic digesters supplied with biochar

Heitkamp

Latorre‐Pérez²,

Nefigmann³

et al. 2021

Preprint

View full text Add to dashboard Cite

show abstract

“…The dismutation pathway (Equation ) is functioned by Smithella species (Xia et al., 2019). Smithella ‐dominated propionate‐oxidizing communities were also promoted by conductive materials, for example, magnetite and carbon nanotubes (Xia et al., 2019), activated carbon (Xu, Han, Zhang, He, & Liu, 2018), biochar (Zhao, Zhang, Holmes, et al, 2016), and graphite felt (Zhang, Ma, et al, 2019). It was proposed that magnetite particles promote DIET by substitute the outer‐surface c‐type cytochromes, while conductive carbon materials provide surface for microbial attachment and act as electron conduits for electron transfer (Lovley, 2017).…”

Section: Introductionmentioning

confidence: 99%

Impacts of conductive materials on microbial community during syntrophic propionate oxidization for biomethane recovery

Guo

Zhang

et al. 2020

Water Environment Research

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Propionate is one of the most important intermediates in anaerobic digestion, and its degradation requires a syntrophic partnership between propionate-oxidizing bacteria and hydrogenotrophic methanogens. Anaerobic digestion efficiency can be improved by direct interspecies electron transfer (DIET) through conductive materials. This study aimed to investigate the effects of DIET on syntrophic propionate oxidization under room temperature (20°C) and reveal the syntrophic partners. Firstly, conventional anaerobic consortium and conductive material-enriched consortium were tested for DIET under high H 2 partial pressure. The latter supplemented with granular activated carbon (GAC) can mitigate H 2 inhibition through DIET. Secondly, a DIET consortium was enriched for testing GAC and magnetite, both showed DIET facilitation. Microbial communities in GAC-and magnetite-supplemented reactors were similar. Syntrophic propionate-oxidizing bacteria, for example, Smithella (3.9%-9.9%) and a genus from the family Syntrophaceae (1.9%-3.6%) and methanogens Methanobacterium (30.3%-75.2%), Methanolinea (8.5%-25.2%), Methanosaeta (11.4%-36.7%), and Candidatus Methanofastidiosum (3.6%-6.6%), were predominant. Functional genes for cell mobility and membrane transport (3.3% and 9.5% in control reactor) increased with GAC (3.7% and 11.1%, respectively) and magnetite (3.7% and 10.9%, respectively) addition. Syntrophic propionate-oxidizing bacteria and methanogenesis partners were revealed by co-occurrence network, for example, Methanobacterium with Smithella, Syntrophobacter, Dechloromonas, and Trichococcus, signifying the importance of the syntrophic partnership in DIET environment.

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“…The antibiotic nalidixic acid was chosen as a stressor, as antibiotics are known to manipulate anaerobic process performance and the involved microbiomes (Mitchell et al, 2013;Mustapha et al, 2016;Bay et al, 2019;Fáberová et al, 2019). GABA was chosen, as high concentrations of butyric acid (an intermediate product from GABA degradation) is known to inhibit syntrophic metabolism in anaerobic digesters (Henson et al, 1985;Zhang et al, 2019).…”

Section: Methane Production Upon Addition Of Microbial Stressorsmentioning

confidence: 99%

Chemically Stressed Bacterial Communities in Anaerobic Digesters Exhibit Resilience and Ecological Flexibility

et al. 2020

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Anaerobic digestion is a technology known for its potential in terms of methane production. During the digestion process, multiple metabolites of high value are synthesized. However, recent works have demonstrated the high robustness and resilience of the involved microbiomes; these attributes make it difficult to manipulate them in such a way that a specific metabolite is predominantly produced. Therefore, an exact understanding of the manipulability of anaerobic microbiomes may open up a treasure box for bio-based industries. In the present work, the effect of nalidixic acid, γ-aminobutyric acid (GABA), and sodium phosphate on the microbiome of digested sewage sludge from a water treatment plant fed with glucose was investigated. Despite of the induced process perturbations, high stability was observed at the phylum level. However, strong variations were observed at the genus level, especially for the genera Trichococcus, Candidatus Caldatribacterium, and Phascolarctobacterium. Ecological interactions were analyzed based on the Lotka-Volterra model for Trichococcus, Rikenellaceae DMER64, Sedimentibacter, Candidatus Cloacimonas, Smithella, Cloacimonadaceae W5 and Longilinea. These genera dynamically shifted among positive, negative or no correlation, depending on the applied stressor, which indicates a surprisingly dynamic behavior. Globally, the presented work suggests a massive resilience and stability of the methanogenic communities coupled with a surprising flexibility of the particular microbial key players involved in the process.

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Synergetic promotion of direct interspecies electron transfer for syntrophic metabolism of propionate and butyrate with graphite felt in anaerobic digestion

Cited by 74 publications

References 36 publications

Monitoring of seven industrial anaerobic digesters supplied with biochar

Monitoring of seven industrial anaerobic digesters supplied with biochar

Impacts of conductive materials on microbial community during syntrophic propionate oxidization for biomethane recovery

Chemically Stressed Bacterial Communities in Anaerobic Digesters Exhibit Resilience and Ecological Flexibility

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