2017
DOI: 10.1016/j.ultsonch.2016.08.029
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Synergetic effects of ultrasound and slightly acidic electrolyzed water against Staphylococcus aureus evaluated by flow cytometry and electron microscopy

Abstract: This study evaluated the synergetic effects of ultrasound and slightly acidic electrolyzed water (SAEW) on the inactivation of Staphylococcus aureus using flow cytometry and electron microscopy. The individual ultrasound treatment for 10min only resulted in 0.36logCFU/mL reductions of S. aureus, while the SAEW treatment alone for 10min resulted in 3.06logCFU/mL reductions. The log reductions caused by combined treatment were enhanced to 3.68logCFU/mL, which were greater than the sum of individual treatments. T… Show more

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Cited by 106 publications
(58 citation statements)
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“…[21] Blank CH showed great bacterial growth inhibition against Staphylococcus aureus and Pseudomonas sp. Meanwhile, Li et al [22] confirmed that EOW was an effective disinfectant, even at low ACC.…”
Section: Assay Of Tvcmentioning
confidence: 99%
“…[21] Blank CH showed great bacterial growth inhibition against Staphylococcus aureus and Pseudomonas sp. Meanwhile, Li et al [22] confirmed that EOW was an effective disinfectant, even at low ACC.…”
Section: Assay Of Tvcmentioning
confidence: 99%
“…Data acquisition was set to 10,000 events at a low flow rate, corresponding to 400–600 events/s. The Kaluza software package (Beckman Coulter) was used to analyze FCM data (Li, Ding, et al, ).…”
Section: Methodsmentioning
confidence: 99%
“…Unfortunately, the benchmark method for determination of viability (the ability to self‐replicate in culture media) is plate count method, which had been used for numerous studies in food industry (Li, Ding, et al, ; Li, Suo, et al, ; Liao et al, ). However, this method was retrospective and time wasting.…”
Section: Introductionmentioning
confidence: 99%
“…TEM analysis was carried out using the method based on a previous study (Li et al ., ). The samples were centrifuged (8000 g , 5 min, 4 °C), and the residues were collected and resuspended in 2 mL of glutaraldehyde (2.5% in phosphate buffer, pH 7.4) overnight at 4 °C.…”
Section: Methodsmentioning
confidence: 99%