Synergetic effects of nitrogen depletion, temperature, and light on the content of phenolic compounds and gene expression in leaves of tomato

Løvdal, Trond; Olsen, Kristine M.; Verheul, Michel; Lillo, Cathrine

doi:10.1016/j.phytochem.2009.12.014

Cited by 227 publications

(148 citation statements)

References 39 publications

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“…These results imply that lower temperature improves the expression of PAL gene, while soil moisture content has a negligible effect on its expression, as evident by the clear relation between PAL expression and the biosynthesis of flavonoid. Similar observations of PAL increased expression were reported after exposure to low temperature in Arabidopsis thaliana leaves [30] and tomato (Solanum lycopersicum) plants [31]. Additionally, the unclear role of moisture effect on PAL gene expression was also supported by the conflicting observations in Camellia sinensis [32] and Scutellaria baicalensis [33] which showed decreased and increased PAL expression under water deficit conditions, respectively.…”

Section: Discussionsupporting

confidence: 71%

“…Similar results were reported in grape skin (V. labruscana) [7] and tomato plants (Solanum lycopersicum cv. Suzanne) [31] showing increased CHS expression in response to lower temperatures. Our results also demonstrated the presence of consistent relationship between soil moisture and C4H expression and flavonoid content (i.e., higher expression under lower soil moisture) as well as the lack of relationship for CHS as expression was higher under high soil moisture, results are at variance with what has been reported in Camellia sinensis [32] and Scutellaria baicalensis [33] in which increased expression was reported under water deficit.…”

Section: Discussionmentioning

confidence: 99%

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Role of Temperature and Soil Moisture Conditions on Flavonoid Production and Biosynthesis-Related Genes in Ginkgo (Ginkgo biloba L.) Leaves

Wang¹,

Cao²,

Wang³

et al. 2015

Nat Prod Chem Res 2015

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Section: Discussionsupporting

confidence: 71%

Section: Discussionmentioning

confidence: 99%

Role of Temperature and Soil Moisture Conditions on Flavonoid Production and Biosynthesis-Related Genes in Ginkgo (Ginkgo biloba L.) Leaves

Wang¹,

Cao²,

Wang³

et al. 2015

Nat Prod Chem Res 2015

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“…Their concentrations fluctuate greatly in response to cellular circumstances such as high-light stress, especially UV-B light, and biotic stress during the defense against herbivores and pathogens (Harborne and Williams, 2000;Peters and Constabel, 2002;Agati and Tattini, 2010;Løvdal et al, 2010;Samanta et al, 2011;Wu et al, 2012) or abiotic stresses such as mineral nutrient depletion (Watanabe et al, 2010). Anthocyanin accumulation occurs in senescing leaves prior to chlorophyll breakdown (Feild et al, 2001).…”

Section: Carbon-based Secondary Metabolites Under the Regulation Of Nmentioning

confidence: 99%

Comprehensive Dissection of Spatiotemporal Metabolic Shifts in Primary, Secondary, and Lipid Metabolism during Developmental Senescence in Arabidopsis

et al. 2013

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Developmental senescence is a coordinated physiological process in plants and is critical for nutrient redistribution from senescing leaves to newly formed sink organs, including young leaves and developing seeds. Progress has been made concerning the genes involved and the regulatory networks controlling senescence. The resulting complex metabolome changes during senescence have not been investigated in detail yet. Therefore, we conducted a comprehensive profiling of metabolites, including pigments, lipids, sugars, amino acids, organic acids, nutrient ions, and secondary metabolites, and determined approximately 260 metabolites at distinct stages in leaves and siliques during senescence in Arabidopsis (Arabidopsis thaliana). This provided an extensive catalog of metabolites and their spatiotemporal cobehavior with progressing senescence. Comparison with silique data provides clues to source-sink relations. Furthermore, we analyzed the metabolite distribution within single leaves along the basipetal sink-source transition trajectory during senescence. Ceramides, lysolipids, aromatic amino acids, branched chain amino acids, and stressinduced amino acids accumulated, and an imbalance of asparagine/aspartate, glutamate/glutamine, and nutrient ions in the tip region of leaves was detected. Furthermore, the spatiotemporal distribution of tricarboxylic acid cycle intermediates was already changed in the presenescent leaves, and glucosinolates, raffinose, and galactinol accumulated in the base region of leaves with preceding senescence. These results are discussed in the context of current models of the metabolic shifts occurring during developmental and environmentally induced senescence. As senescence processes are correlated to crop yield, the metabolome data and the approach provided here can serve as a blueprint for the analysis of traits and conditions linking crop yield and senescence.

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“…The flavonoid enzymes in Arabidopsis are encoded by single genes (Shirley et al, 1995;Winkel, 2006), but in tomato, several of the enzymes are encoded by multiple genes. The Sol Genomics Network database contains four putative copies of CHS (SGN-U579222, SGN-U580856, SGN-U581366, and SGN-U580262), with CHS2 (SGN-U580856) being the most highly expressed (Løvdal et al, 2010). There are two and three putative copies of CHI (SGN-U577427 and SGN-U579009) and FLAVO-NOID 39-HYDROXYLASE (F39H; SGN-U576659, SGN-U573255, and SGN-U570072), respectively.…”

Section: Examination Of Genes Encoding Flavonoid Biosynthetic Pathwaymentioning

confidence: 99%

The anthocyanin reduced Tomato Mutant Demonstrates the Role of Flavonols in Tomato Lateral Root and Root Hair Development

2014

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This study utilized tomato (Solanum lycopersicum) mutants with altered flavonoid biosynthesis to understand the impact of these metabolites on root development. The mutant anthocyanin reduced (are) has a mutation in the gene encoding FLAVONOID 3-HYDROXYLASE (F3H), the first step in flavonol synthesis, and accumulates higher concentrations of the F3H substrate, naringenin, and lower levels of the downstream products kaempferol, quercetin, myricetin, and anthocyanins, than the wild type. Complementation of are with the p35S:F3H transgene reduced naringenin and increased flavonols to wild-type levels. The initiation of lateral roots is reduced in are, and p35S:F3H complementation restores wild-type root formation. The flavonoid mutant anthocyanin without has a defect in the gene encoding DIHYDROFLAVONOL REDUCTASE, resulting in elevated flavonols and the absence of anthocyanins and displays increased lateral root formation. These results are consistent with a positive role of flavonols in lateral root formation. The are mutant has increased indole-3-acetic acid transport and greater sensitivity to the inhibitory effect of the auxin transport inhibitor naphthylphthalamic acid on lateral root formation. Expression of the auxin-induced reporter (DR5-b-glucuronidase) is reduced in initiating lateral roots and increased in primary root tips of are. Levels of reactive oxygen species are elevated in are root epidermal tissues and root hairs, and are forms more root hairs, consistent with a role of flavonols as antioxidants that modulate root hair formation. Together, these experiments identify positive roles of flavonols in the formation of lateral roots and negative roles in the formation of root hairs through the modulation of auxin transport and reactive oxygen species, respectively.

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Synergetic effects of nitrogen depletion, temperature, and light on the content of phenolic compounds and gene expression in leaves of tomato

Cited by 227 publications

References 39 publications

Role of Temperature and Soil Moisture Conditions on Flavonoid Production and Biosynthesis-Related Genes in Ginkgo (Ginkgo biloba L.) Leaves

Role of Temperature and Soil Moisture Conditions on Flavonoid Production and Biosynthesis-Related Genes in Ginkgo (Ginkgo biloba L.) Leaves

Comprehensive Dissection of Spatiotemporal Metabolic Shifts in Primary, Secondary, and Lipid Metabolism during Developmental Senescence in Arabidopsis

The anthocyanin reduced Tomato Mutant Demonstrates the Role of Flavonols in Tomato Lateral Root and Root Hair Development

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